Figure 2
From: Involvement of TRPA1 activation in acute pain induced by cadmium in mice
Inhibition of Cd-induced [Ca2+] i increase by removal of extracellular Ca and TRPA1 blockers. (A) [Ca2+]i responses in mouse DRG neurons induced by repetitive stimuli with Cd (30 μM, 1 min) with an interval of 30 min. (B) Removal of extracellular Ca, (C) application of ruthenium red (10 μM) or (D) HC-030031(10 μM) was performed before 2 min, during and after 2 min application of Cd. Traces shows mean ± SEM of representative data from 8–17 cells. (E) Summarized effects of these pharmacological treatments. S1 and S2 are the first peak amplitude and the second one without (No blocker) and with these treatments, respectively. Data are shown as S2/S1. (No blocker; n=13, Ca0; n=29, ruthenium red; n=39, HC-030031; n=16, AP18 (10 μM); n=22, A967079 (10 μM); n=18, BCTC (10 μM); n=32, from 3 mice) **,P<0.01 vs. No blocker.