In naïve pain-free mice, SNC80 shows minimal Ca2+channel inhibition in DRG neurons and does not alter the response threshold to von Frey hair stimulation. A. SNC80 applied to dissociated DRG neurons from adult mice showed minimal VDCC inhibition equally in β-arrestin 2 WT or KO mice (F1,41 = 1.66). Exemplar currents show VDCCs before (1), during (2) and after (3) SNC80 (1μM) application, vertical scalebar = 20 ms and horizontal scalebar = 0.5 pA, n = 20–22. B. β-arrestin 2 WT and KO mice were tested with vehicle or SNC80 (10 mg/kg, i.p.), and mechanical responses were assessed 45 min later. Baseline mechanical responses are represented by the dashed line. There was no significant effect of SNC80 in either genotype, as determined by 2-way ANOVA (n = 4-5mice/group).