Figure 2
In wildtype mice CFA increased SNC80-Ca2+channel inhibition and reversed CFA-induced allodynia. A. CFA induced an increase in SNC80-VDCC inhibition in WT DRG neurons from the ipsi-, but not contra-lateral sides to the CFA injection; ANOVA: F (8,78) = 14.86, ** p < 0.01 and *** p < 0.001 vs. basal/naive inhibition, shown by the dashed line. An exemplar current shows VDCCs before (1), during (2) and after (3) SNC80 (1 μM) application, vertical scalebar = 20 ms and horizontal scalebar = 0.5 pA, n = 6–20. B. The effect of SNC80 (10 mg/kg i.p.) or saline was assessed in mice which had been treated with CFA 2, 3, 7 and 14 days prior to the test. Baseline mechanical responses are represented by the dashed line. *** p < 0.001 as determined by 2-way ANOVA with a Holm-Sidak post-hoc analysis (n = 4–8 mice/group).