Figure 4

Inflammatory pain altered VDCC-density and current-type contribution in β-arrestin 2 knockout, but not wildtype mice. A and B. The current–voltage relationship recorded from either WT (A) or KO (B) neurons was not altered by CFA (3 days post-injection). Exemplar currents from WT and KO neurons of the contralateral (non-CFA) and ipsilateral (CFA) sides are shown above the current–voltage graphs depicting the maximum current induced by each voltage and corrected for cell capacitance. Vertical scalebar =20 ms and horizontal scalebar = 0.5 pA, n = 10–16. C and D. The contribution of N-type VDCCs to the total current in WT neurons was not altered by CFA (C) but was reduced in DRG neurons from KO mice (D; F _(3,25) = 4.57, n = 6–7). Exemplar currents show VDCCs measured in the absence (1) and presence (2) of the selective N-type inhibitor, ϖ -Conotoxin GV1A (10 μM). * p < 0.05.