Figure 2

Mechanical hypersensitivity and guarding behavior is prevalent after skin plus deep incision with genetic knockout and pharmacological inhibition of TRPA1. a. Response frequency to 11.2mN filament on POD1 for skin plus deep incised WT, TRPA1 KO, WT treated with vehicle and WT treated with TRPA1 antagonist, HC-030031. There is no difference in mechanical hypersensitivity after skin plus deep incision with genetic knockout or pharmacological block of TRPA1. 5 mice per each WT and TRPA1 KO groups; 8 mice per each WT groups treated with either vehicle or HC-030031. b. Guarding nocifensive (non-evoked) behavior on POD1 for naïve, skin-only and skin plus deep incised mice. There is no difference in pain score between naïve and skin-only incised mice. Both WT and TRPA1 KO skin plus deep incised mice exhibit increased pain scores, compared to naïve and skin-only incised mice (p < 0.0001; **p < 0.001; applies to comparison to both naïve and skin-only). 14 naïve; 7 WT skin-only incision mice. The skin plus deep incised mice are the same as those used in Figure 2a.