Figure 5

Skin-only incision induced thermal hypersensitivity is dependent on TRPV1. a. Heat behavior assay for WT skin-only incised and sham mice. WT mice exhibit decreased paw withdrawal latencies on POD1 after skin-only incision injury as compared to sham controls (p = 0.0013; ***p < 0.0001). 8 mice per group. b. Heat behavior assay for WT and TRPV1 KO skin-only incised mice. TRPV1 KO skin-only incised mice exhibit significantly longer paw withdrawal latencies at POD1 than skin incised WT mice (2-way repeated measures ANOVA: p < 0.0001; ***p < 0.0001). TRPV1 mice exhibit an increase in heat sensitivity compared to baseline (paired t-test *p = 0.0022); however, WT mice exhibit a much greater increase in heat sensitivity compared to their baseline values (paired t-test ***p < 0.0001). 7 mice per group. Since multiple statistical tests were conducted, we used assumed p-values <0.025 were significant rather than 0.05. Through nonparametric testing we reached the same conclusion. c. Response frequency to 11.2mN filament for WT and TRPV1 KO mice following skin-only incision injury. There was no difference at baseline or POD1 between WT and TRPV1 KO mice (2-way repeated measures ANOVA: p > 0.05; n.s.). Both WT and TRPV1 KO mice exhibit increase mechanical sensitivity on POD1 following skin-only incision injury compared to their respective baseline values (paired t-tests; ***p = 0.0002; **p = 0.0003). Same mice as those in Figure 5b. Since multiple statistical tests were conducted, we assumed p-values <0.025 were significant rather than 0.05. Through nonparametric testing we reached the same conclusion.