Effects of ZBD-2 on pain perception. Mechanical allodynia and thermal hyperalgesia were detected on Day 0, 1, 7, 14 and 21 after CFA injection. ZBD-2 (0.15 and 1.5 mg/kg) did not change the mechanical allodynia in the ipsilateral (A) and contralateral hindpaw (B). ZBD-2 (0.15 and 1.5 mg/kg) did not change the thermal hyperalgesia in the ipsilateral (C) and contralateral hindpaw (D). (E) ZBD-2 did not reduce edema in CFA-injected hindpaws. n = 6 in each group, **p < 0.01 compared to the control group.