Figure 6

The effect of S4IL4 or SHZ on the expression of spinal TNFα, IL-1β, and p-p38. Rats were inoculated with S4IL4 or SHZ. Rats received a repeated morphine dose at 1 week after vector inoculation. In the sham group, rats received SHZ and a repeated dose of saline. At day 7, 1 hour after the last dose of morphine or saline, L4/5 spinal cord dorsal horns were harvested, and the expression of TNFα, IL-1β, and p-p38 were tested using Western blots. Repeated morphine administration significantly induced upregulation of spinal TNFα (A), IL-1β (B), and p-p38 (C). The expression of spinal TNFα, IL-1β, and p-p38 in morphine tolerance rats with S4IL4, was significantly lower than that in morphine tolerance rats with SHZ. *P < 0.05 vs sham, # P < 0.05 vs SHZ/MT, n = 3-5, ANOVA, post hoc comparisons using Fisher’s PLSD test (StatViewJ 5.2).