Figure 2
From: Polysulfide evokes acute pain through the activation of nociceptive TRPA1 in mouse sensory neurons
Polysulfide-responsive neurons highly correspond to TRPA1 agonist-sensitive ones. (A) Actual recordings of [Ca2+]i responses to sequential application of Na2S3 (10 μM), allylisothiocyanate (AITC, 0.3 mM), capsaicin (Cap, 1 μM), and KCl (K, 80 mM). (B) An image under transmitted light, and pseudocolor images; before (Pre) and after the application of Na2S3 (+Na2S3), allylisothiocyanate (+AITC), capsaicin (+Cap), and KCl (+K). In a bright field image, cells with arrows (1–3) correspond to (A). (C) Venn diagram showing the sensitivities to Na2S3, AITC, capsaicin, and KCl (n = 322 from five mice). Numbers indicate the number of cells responding to each stimulus. A number in the outermost frame expresses the number of neurons responding to KCl alone. Note that Na2S3-responding neurons are mostly coincident with AITC-responding ones.