Figure 5
From: Sodium channel Nav1.7 in vascular myocytes, endothelium, and innervating axons in human skin
Digital fluorescence images of Nav1.7 (red) and PGP 9.5 (green) immunolabeling (IL) in the epidermis (Ep) and upper dermis (UD) biopsies of normal human palmar glabrous skin (A, Abcam anti-Nav1.7) and normal human plantar glabrous skin (B, Alomone anti-Nav1.7). Stratum corneum, sc; stratum granulosum, sg; stratum spinosum (ss); stratum basalis (sb), dermal papilla (dp). Straight arrows indicate epidermal sensory endings, curved arrows indicate small nerves and individual axons or endings in the upper dermis. The areas enclosed in the large rectangles are 2X enlargements of those in the smaller rectangles. Of all the innervation revealed by anti-PGP 9.5, only some express Nav1.7-IL (yellow straight and curved arrows) whereas other only express PGP 9.5-IL (green straight and curved arrows). Aβ-fiber innervation of a Meissner corpuscle (MC) has little if any Nav1.7-IL. Kertinocytes especially in stratum granulosum label for Nav1.7 (arrowheads) which has a more membranous distribution with the Alomone anti-Nav1.7 antibody, but more diffuse labeling with the Abcam anti-Nav1.7 antibody. Scale bar = 100 μm.