Minocycline has no effect on post-LTP induction in the ACC. A and B: one sample of 64-channel recordings of cingulate post-LTP induced in the presence of minocycline (Mino, 100 μM). A, baseline; B, 2 h after TBS. After stabilizing the baseline responses for 30 min, minocycline was bath applied from 30 min before till 30 min after delivery of TBS to the deep layer. Minocycline could not prevent the post-LTP induction. The red filled circle denotes the stimulated channel (Ch. 21). Vertical lines demarcate specific layers. C: results of one channel (Ch. 24) showing the normal induction of post-LTP by TBS. D: summary of averaged data from 6 superficial channels of 1 slice. E: pooled data of the superficial layer from 7 slices of 7 mice. F: pooled data of the deep layer from 6 slices of 6 mice. Insets in C and F are sample traces at the time points indicated by numbers in the graph. Calibration: 100 μV, 10 ms. Arrows in C-F indicate starting point of TBS delivery. Error bars in E and F represent SEM.