Figure 3

Double immunostaining of DβH and pCREB in representative bilateral LC tissues. a–c Naïve rat, contralateral side; j–l naïve rat, ipsilateral side; d–f SNL rat, contralateral side; m–o SNL rat, ipsilateral side; g–i SNL+SCS rat, contralateral side; p–r SNL+SCS rat, ipsilateral side. s Quantitative summary of pCREB-positive neurons in bilateral LC regions. No significant difference in the number of pCREB-positive neurons was noted among the groups. t Quantitative summary of the ipsilateral-to-contralateral ratio of pCREB-positive neurons and DβH immunoreactivity in bilateral LC regions. Quantitative data were obtained from three consecutive slices of four animals per group. Data are mean ± SEM; *p < 0.05 and **p < 0.01 by the Tukey–Kramer test.