Figure 1

Intrathecal injection of non-selective CaCCs inhibitors reduces tactile allodynia. Time-course of the antiallodynic effect of NPPB (300 μg, a), 9-AC (300 μg, c) and NFA (300 μg, e) in rats subjected to L5/L6 spinal nerve ligation. Withdrawal threshold was assessed 14 days after spinal nerve injury. Dose–response relationship of the antiallodynic effect of NPPB (30–300 μg, b), 9-AC (10–300 μg, d) and NFA (10–300 μg, f) in spinal nerve injured rats compared to sham (S) and vehicle (V) groups. Data are presented as the mean ± SEM for six animals. Note that non-selective CaCCs inhibitors significantly increased withdrawal threshold as well as the % of maximum possible effect (%MPE). *Significantly different from the vehicle group (p < 0.05), as determined by one-way ANOVA followed by the Student–Newman–Keuls test.