Figure 7

The two GIRKs overlap with SST2A and VGLUT1 in control spinal dorsal horn. A GIRK1-LI overlaps with SST2A-LI. B–B3 Triple-staining (B3) for GIRK1 (B), SST2A (B1) and Hoechst (B2) shows no co-existence of GIRK1-LI (solid arrowheads) with SST2A-LI (open arrowheads) in the superficial laminae. C GIRK1-LI overlaps with VGLUT1-LI in the superficial laminae. D–D2 Double-staining (D2) shows that only a few VGLUT1-IR boutons (D2) are GIRK1⁺ (D, D2) (open arrowheads), as indicated in the high power magnification (upper box, D2). Most of VGLUT1⁺ boutons have no GIKR1-LI (solid arrowheads). E GIRK2-LI overlaps with SST2A-LI in superficial laminae. F–F3 Triple-staining (F3) for GIRK2 (F), SST2A (F1) and Hoechst (F2) shows a possible membrane associated co-localization of GIRK2 and SST2A (solid arrowheads), open arrowheads show the GIRK2⁺, but SST2A-negative (⁻) neurons. (G) GIRK2-LI overlaps with VGLUT1-LI. (H–H2) Double-staining (H2) for GIRK2 (H) and VGLUT1 (H1) shows that some VGLUT1⁺ boutons are GIRK2⁺ in the spinal dorsal horn (open arrowheads), as shown in high power magnification (upper box, H2). Most of VGLUT1⁺ boutons are however GIRK2⁻ (solid arrowheads). Scale bars indicate 100 μm (A, C, E, G), 25 μm (D–D2, H–H2), 20 μm (B–B3), 10 μm (F–F3).