Protein and mRNA expression of GIRK1 in DRGs and spinal cord 14 days after axotomy. A, B Percentage of GIRK1+ NPs is significantly decreased in the ipsilateral (n = 8) as compared to contralateral DRGs (n = 8). C Western blot result showing GIRK1 protein levels confirms this effect (n = 4 per group). D GIRK1 mRNA is extensively distributed in the contralateral DRG and is ipsilaterally down-regulated after axotomy (upper panel: low magnification; lower panel: high magnification). E GIRK1-LI (within white frame) in the dorsal horn. F Monitoring fluorescence intensity in the dorsal horn shows down-regulation of GIRK1-LI in all animals (n = 3 per group). *P < 0.05. Scale bars indicate 500 μm (E), 200 μm (D, upper panel; A) and 100 μm (D, lower panel).