Fig. 2

B1R is up regulated in skin tissues of DCP-treated group. Western blot (a) and RT-PCR (b) analysis of B1R in skin tissues obtained from sham (control) and DCP-treated mice. Protein expression (a) and PCR product (b) obtained with the specific B1R and β-tubulin antibodies or B1R and GAPDH primers. Insets (a upper panels) show a representative band of the protein expressed in both groups. Data were normalized against β-tubulin or GAPDH and expressed as the mean ± SEM of five independent mice. **P < 0.01 by the Student t test