Figure 5

Intracellularly stained and physiologically characterized sensory neurons in naïve mice from the ex vivo preparation. A neurobiotin stained “A”-fiber neuron (arrows) from a P7 mouse (A) that responded to mechanical stimuli and heat stimuli (D) was found to be immunoreactive for both TRPV1 (B; red) and ASIC3 (C; blue). One “C”-fiber neuron (arrows) recovered from a P8 mouse that was intracellularly stained with neurobiotin (E) and responded to mechanical deformation of the skin but not heat stimuli (H) was found to be immunonegative for TRPV1 (F; red) and did not bind isolectin B4 (IB4; G; blue). A polymodal (L) “C”-fiber neuron intracellularly stained with neurobiotin (I) and recovered from a P21 naïve ex vivo preparation (arrows) was found to be TRPV1 immunonegative (J; red), but did bind IB4 (K; blue). Scale bar for images: 40 μm.