Age-dependent sensitization of cutaneous nociceptors during developmental inflammation
© Jankowski et al.; licensee BioMed Central Ltd. 2014
Received: 28 March 2014
Accepted: 22 May 2014
Published: 7 June 2014
It is well-documented that neonates can experience pain after injury. However, the contribution of individual populations of sensory neurons to neonatal pain is not clearly understood. Here we characterized the functional response properties and neurochemical phenotypes of single primary afferents after injection of carrageenan into the hairy hindpaw skin using a neonatal ex vivo recording preparation.
During normal development, we found that individual afferent response properties are generally unaltered. However, at the time period in which some sensory neurons switch their neurotrophic factor responsiveness, we observe a functional switch in slowly conducting, broad spiking fibers (“C”-fiber nociceptors) from mechanically sensitive and thermally insensitive (CM) to polymodal (CPM). Cutaneous inflammation induced prior to this switch (postnatal day 7) specifically altered mechanical and heat responsiveness, and heat thresholds in fast conducting, broad spiking (“A”-fiber) afferents. Furthermore, hairy skin inflammation at P7 transiently delayed the functional shift from CM to CPM. Conversely, induction of cutaneous inflammation after the functional switch (at P14) caused an increase in mechanical and thermal responsiveness exclusively in the CM and CPM neurons. Immunocytochemical analysis showed that inflammation at either time point induced TRPV1 expression in normally non-TRPV1 expressing CPMs. Realtime PCR and western blotting analyses revealed that specific receptors/channels involved in sensory transduction were differentially altered in the DRGs depending on whether inflammation was induced prior to or after the functional changes in afferent prevalence.
These data suggest that the mechanisms of neonatal pain development may be generated by different afferent subtypes and receptors/channels in an age-related manner.
The contribution of sensory neurons to pain development has been extensively documented in adult animals (refs rev. in ). However, the role that sensory neurons play in pain development during early life is not as well understood. Many studies have analyzed sensory neurons in developing animals, but have typically analyzed dissociated neurons in response to various stimuli [2–8], or studied the changes in neurochemical or anatomical aspects of dorsal root ganglia (DRGs; [9–13]) in relation to pain behaviors [14, 15] after injuries.
Some studies have evaluated sensory function with in vitro or in vivo systems in neonates [16–21]. These data have suggested that in uninjured mice, the individual subpopulations of sensory neurons mimic the functional response properties of their adult equivalents. Thus it could be suggested that sensitization of sensory neurons in neonates in response to peripheral damage would be similar as adults. However, it is well known that target derived neurotrophic factors are vital for the proper development of primary afferents and many neonatal sensory neurons switch their neurotrophic factor responsiveness from nerve growth factor (NGF) to glial cell line-derived neurotrophic factor (GDNF) during postnatal development [22–24]. Specifically, NGF has been shown to be crucial for the development of myelinated nociceptors [25–27] and may play a role in C-fiber heat responsiveness . GDNF signaling has also been linked to the development of heat responsiveness in mouse sensory neurons , and may in fact be regulated by early NGF signaling . In addition, it has been demonstrated that there is an early postnatal loss of heat sensitivity in myelinated sensory neurons during the first two weeks of life .
Therefore, injury at different stages of postnatal development may actually lead to distinct changes in sensory neurons compared to what is observed in adult animals. In support of this notion, one study that analyzed the functional response properties of developing sensory neurons in vivo during skin incision  showed that there were differences in mechanical sensitivity during neonatal injury that were not observed in older mice . Nevertheless, there has not been a comprehensive analysis of the plasticity that occurs in sensory afferents after injury induced at different stages of postnatal development that span the period in which sensory afferents are thought to experience normal phenotypic switching [22, 24]. We thus first sought to functionally define sensory neurons during this critical period of postnatal development in order to determine whether there were any functional correlates to known changes in afferent phenotype. Then we tested the hypothesis that peripheral injury during early life and prior to these phenotypic switches uniquely alters sensory neuron responsiveness compared to injury following the second week of life and after the observed switching. To test this, we used a neonatal ex vivo recording preparation to analyze the comprehensive phenotypes of individual afferents in mice with peripheral inflammation induced at either P7 or P14 by injection of carrageenan into the hairy hindpaw skin. Changes in afferent function were then compared to changes in mRNA and protein expression in the DRGs to determine potential mechanisms for the observed alterations in function. We have found that hairy skin inflammation induced at P7 distinctly alters sensory neuron responsiveness with corresponding upregulation of specific receptors/channels in the DRGs involved in sensory transduction compared to P14 inflammation. P7 inflammation specifically was also found to delay normal changes in sensory neuron prevalence at the same time they are thought to undergo phenotypic switches [22, 24].
Response properties of cutaneous afferents during postnatal development
To confirm earlier reports (e.g. [19, 21, 30]) and first determine any age-related changes in peripheral response properties of individual cutaneous afferents in neonatal mice, we performed single unit recording with our neonatal ex vivo hairy skin/saphenous nerve/DRG/spinal cord preparation in uninjured mice from postnatal day seven (P7) through P21 (see Methods for information on animal numbers and average afferents recorded per group/condition). Two clear categories of sensory afferents were detected based on conduction velocity (CV) within each experiment. Fast conducting “A”-fibers had at least double the CVs as slowly conducting “C”-fibers in each preparation (Figure 1A; ). Overall, “C”-fibers had a mean CV = 0.53 m/s. (<P10 Ave. = 0.54 m/s; ≥P10 Ave. = 0.53 m/s) while “A”-fibers had a mean CV = 3.26 m/s (<P10 Ave. = 2.01 m/s; ≥P10 Ave. = 3.72 m/s). These two CV classifications could then be further subdivided based on spike width (narrow vs. broad) and then separated based on response characteristics. Both fast (“A”-fibers) and slowly (“C”-fibers) conducting, narrow spiking afferents were found to have no differences in peripheral response properties (mechanical or thermal) at any of the ages tested (not shown), including after inflammation, similar to previous reports . These included cells with “A”-fiber CVs that displayed rapidly adapting and slowly adapting type I responses, and “C”-fibers that were either mechanically sensitive and cold/cooling sensitive or mechanically insensitive and cold/cooling sensitive. Therefore, the remainder of this report will focus on the broad spiking afferent subclasses (putative nociceptors).
When specifically analyzing the afferents with fast CVs and broad spikes, we found that the responses to mechanical stimuli were not different between the mechanically sensitive, thermally insensitive (AM), and mechanically and heat (and sometimes cold) sensitive (polymodal) fibers (APM); therefore, data analyzing response properties from these cell types was combined for ease of presentation hereafter. The AMs/APMs displayed no overt changes in mechanical thresholds (P7: 16.3 mN ± 9.2 mN; P8: 55 mN ± 45 mN; P10: 75 mN ± 25 mN; P14: 21.7 mN ± 14.2 mN; P21: 33.5 mN ± 22.1 mN; p value < 0.3) or mean peak instantaneous frequencies (P7: 75.8 Hz ± 20.4 Hz; P8: 40 Hz ± 18.5 Hz; P10: 65.56 Hz ± 32.4 Hz; P14: 110.7 Hz ± 103.9 Hz; P21: 41.4 Hz ± 16.9 Hz; p value < 0.63) to deformation of the skin (P7: n = 6; P8: n = 5; P10: n = 3; P14: n = 3; P21: n = 6). There were also no changes in those cells with “A”-fiber CVs that responded to heat (P7: n = 3; P8: n = 1; P10: n = 1; P14: n = 2; P21: n = 1) in terms of the thresholds (P7: 48.4°C ± 2.7°C; P8: 44.4°C ± 0°C; P10: 45.7°C ± 0°C; P14: 42.6°C ± 5.3°C; P21: 42.7°C ± 0°C) or firing (P7: 4.6 Hz ± 2.8 Hz; P8: 1.9 Hz ± 0 Hz; P10: 1.7 Hz ± 0 Hz; P14: 1.5 Hz ± 1.3 Hz; P21: 9.2 Hz ± 0 Hz; p value < 0.75) to heat stimuli at any developmental age tested.
Since we obtained low numbers of heat sensitive afferents at some of these developmental time points in naïve mice precluding us from an accurate statistical analysis across ages, we also combined data from ex vivo experiments in mice < P14 (P7-P10) and those ≥ P14 (P14 and P21) to at least determine if there were any changes in heat sensitivity over a larger time period during development. Combined data from these groups again shows no differences in heat thresholds (<P14: 47.1°C ± 1.7°C; n = 5 vs. ≥P14: 42.6°C ± 2.3°C; n = 3; p value < 0.2) or firing to heat stimuli (<P14: 3.5 Hz ± 1.7 Hz vs. ≥P14: 4.0 Hz ± 2.7 Hz; p value < 0.88). These results are consistent with previous reports showing a lack of changes in A-fiber response properties during this period of postnatal development [19, 21, 30]. Finally, only one broad spiking “A”-fiber (detected at P14) had a cold/cooling response out of all naïve “A”-fibers recorded.
Similar results were found in the slowest conducting (“C”-fibers), broad spiking afferents that responded to mechanical and heat stimuli and sometimes cold (“C”-polymodal; CPM; P7: n = 3; P8: n = 6; P10: n = 12; P14: n = 14; P21: n = 22). No changes in mechanical thresholds (P7: 21.7 mN ± 14.2 mN; P8: 18 mN ± 11.1 mN; P10: 8.3 mN ± 3.9 mN; P14: 22.3 mN ± 8.7 mN; P21: 8.5 mN ± 1.8 mN; p value < 0.33) or firing (P7: 52.2 Hz ± 27.7 Hz; P8: 74.2 Hz ± 16 Hz; P10: 38.9 Hz ± 7.2 Hz; P14: 40.7 Hz ± 12.7 Hz; P21: 56.5 Hz ± 6.3 Hz; p value < 0.24) to mechanical stimuli were observed in these fibers over time. In addition, the responsiveness to heat stimuli in these afferents were not different over time (Heat Thresholds: P7: 47.2°C ± 2.5°C; P8: 43.5°C ± 2.7°C; P10: 44.5°C ± 1.4°C; P14: 42.1°C ± 1.6°C; P21: 43.2°C ± 0.8°C; p value < 0.5; Heat Instantaneous Frequencies: P7: 5.0 Hz ± 2.7 Hz; P8: 3.7 Hz ± 1.4 Hz; P10: 17.0 Hz ± 7.9 Hz; P14: 11.2 Hz ± 3.8 Hz; P21: 13.2 Hz ± 3.1 Hz; p value < 0.52). Of the CPM neurons that displayed a cold/cooling response (P7: n = 1; P8: n = 0; P10: n = 3; P14: n = 3; P21: n = 13), no differences were observed over time for these response properties (Cold/Cooling Thresholds: P7: 8.9°C ± 0°C; P8: undetermined; P10: 21.3°C ± 2°C; P14: 10.8°C ± 1.6°C; P21: 11.8°C ± 1.2°C; Cold/Cooling Instantaneous Frequencies: P7: 1.5 Hz ± 0Hz; P8: undetermined; P10: 3.1 Hz ± 1.5 Hz; P14: 3.1 Hz ± 1.9 Hz; P21: 4.5 Hz ± 2.4 Hz). Similar results were obtained if we combined these data into groups < P14 and ≥ P14 (Cold Thresholds: 13.9°C ± 2.1°C vs. 11.6°C ± 1.0°C, n = 4; Cold Firing: 2.1 Hz ± 0.3 Hz vs. 4.0 Hz ± 0.7 Hz, n = 16; p value < 0.4).
The mechanically insensitive, heat sensitive “C”-fiber afferents (CH; P7: n = 2; P8: n = 2; P10: n = 2; P14: n = 4; P21: n = 3) also showed no differences in heat threshold (P7: 44.8°C ± 0.1°C; P8: 43.0°C ± 0°C; P10: 42.9°C ± 0.4°C; P14: 44.7°C ± 2.2°C; P21: 42.8°C ± 2.9°C; p value < 0.92) or firing (P7: 19.1 Hz ± 17.7 Hz; P8: 20.0 Hz ± 0 Hz; P10: 42.8 Hz ± 41.7 Hz; P14: 30.5 Hz ± 8.3 Hz; P21: 9.8 Hz ± 3.8 Hz; p value < 0.76) to heat stimuli at all ages tested. Combining these data into < P14 (n = 6) and ≥ P14 (n = 7) also revealed no changes over time (Heat Thresholds: 43.5°C ± 0.4°C vs. 43.9°C ± 1.6°C, p value < 0.76; Heat Firing: 27.3 Hz ± 12.7 Hz vs. 23.6 Hz ± 6.9 Hz, p value < 0.8). However, one cell type, the mechanically sensitive, thermally insensitive fibers (CM; P7: n = 11; P8: n = 15; P10: n = 5; P14: n = 8; P21: n = 7), showed reduced firing to mechanical stimulation of the skin beginning at P10 (26.9 Hz ± 7.9 Hz; p value < 0.05) relative to both P7 (74.6 Hz ± 8.0 Hz) and P8 (50.6 Hz ± 9.8 Hz) mice. This trend continued at P14 (25.0 Hz ± 7.6 Hz; p value < 0.05) and P21 (18.3 Hz ± 7.5 Hz; p value < 0.05). The mechanical thresholds in these afferents however, were not found to be altered at any age tested (P7: 21.0 mN ± 9.7 mN; P8: 50.6 mN ± 11.7 mN; P10: 48.3 mN ± 25.9 mN; P14: 53.3 mN ± 3.33 mN; P21: 60.6 mN ± 18.2 mN; p value < 0.25).
Percentages of cutaneous afferents during postnatal development
The percentage of AMs and APMs out of all A-fibers recorded; however, showed no differences at any postnatal age tested (AM: P7: 28%, n = 5/18; P8: 40%, n = 4/10; P10: 43%, n = 3/7; P14: 23%, n = 3/13; P21: 42%, n = 5/12; p value < 0.78; APM: P7: 17%, n = 3/18; P8: 10%, n = 1/10; P10: 14%, n = 1/7; P14: 15%, n = 2/13; P21: 8%, n = 1/12; p value < 0.98). Taken together these data suggest that the response properties of individual saphenous afferent subtypes are generally not different during postnatal development, consistent with previous reports utilizing similar cutaneous preparations [19, 30]. However, unlike “A”-fiber subpopulations which appear to display differences in frequency within the first week of life , the prevalence of “C”-fiber subtypes changes significantly during the second week of life (between P8 and P10).
Gene expression patterns in DRGs during postnatal development
Percent changes in mRNA expression in the L2/L3 DRGs of naïve mice
Postnatal Day 7
Postnatal Day 8
Postnatal Day 10
Postnatal Day 14
Postnatal Day 15
Postnatal Day 17
Postnatal Day 21
-55 ± 8*
-57 ± 14*
-63 ± 11*
-74 ± 12*
-72 ± 13*
-78 ± 18*
-84 ± 21*
1 + 20
56 ± 31
75 ± 29*
101 ± 33*
102 ± 41*
49 ± 7*
67 ± 23*
-43 ± 8
-44 ± 15
-58 ± 8*
-72 ± 13*
-71 ± 12*
-71 ± 12*
-76 ± 21*
13 ± 35
14+ ± 5
-7 ± 29
-30 ± 29
-52 ± 13
34 ± 29
-40 ± 24
115 ± 20*
70 ± 21*
53 ± 15*
68 ± 44
62 ± 18*
109 ± 20*
-44 ± 20
-7 ± 24
-14 ± 28
-20 ± 21
-35 ± 18
57 ± 34
-27 ± 19
-45 ± 23
-3 ± 31
-38 ± 29
4 ± 27
-8 ± 27
-21 ± 15
-7 ± 21
-17 ± 24
885 ± 30*
1312 ± 29*
2196 ± 17*
2517 ± 19*
1639 ± 53*
3014 ± 17*
2442 ± 23*
53 ± 9*
57 ± 12*
27 ± 10
-22 ± 10
-41 ± 13
11 ± 19
7 ± 16
-4 ± 20
-44 ± 18
-33 ± 16
-53 ± 17*
-59 ± 23*
-57 ± 17*
-58 ± 18*
206 ± 26*
95 ± 23*
198 ± 18*
104 ± 25*
102 ± 26*
330 ± 27*
240 ± 20*
111 ± 16*
60 ± 20#
60 ± 19#
36 ± 23
34 ± 15
25 ± 25
35 ± 28
60 ± 22#
28 ± 24
80 ± 30
32 ± 22
35 ± 24
42 ± 20
22 ± 20
-7 ± 11
-16 ± 15
-44 ± 7*
-50 ± 10*
-64 ± 13*
-59 ± 6*
-57 ± 18*
25 ± 19
34 ± 19
-17 ± 22
-30 ± 22
-53 ± 18#
-33 ± 15
-31 ± 22
At this same time point (P10), we also found a significant decrease in P2X3 (-44 ± 7%; p value < 0.05) and the artemin neurotrophic factor co-receptor GFRα3 (-58 ± 8%; p value < 0.05). Prior to this time point, there was also significantly increased mRNA levels of the excitability regulator TRPC3 at P7 (53 ± 9%; p value < 0.05) and P8 (57 ± 12%; p value < 0.05) compared to P0 DRGs; however, levels were not found to be different than P0 expression from P10 onward. Finally, we found a significant increase in TRPV1 expression, but only at P7 (111 ± 16%; p value < 0.05). Other interesting changes in expression detected during the first three weeks of life included a significant increase in the Mg2+ channel TRPM6 [34, 35] at P7 (206 ± 26%; p value < 0.05) and all other time-points tested. In addition, we found a highly significant increase in the extreme cold responsive and irritant receptor TRPA1 [36, 37] at P7 (885 ± 30%; p value < 0.05), and this also remained highly elevated thereafter.
Age-related changes in response properties of cutaneous afferents during neonatal inflammation
Since we observed no changes in “A”-fiber prevalence or response properties during the second and third weeks of life, but did observe changes in CM neuron firing and the prevalence of CM and CPM neurons between P8 and P10, we wanted to determine if there were age-specific changes in the response properties of individual afferent subtypes after cutaneous inflammation initiated at time points that spanned this critical period of postnatal sensory neuron development. We therefore performed ex vivo recording analysis in mice one, three and seven days after inflammation of the hairy hindpaw skin induced at either P7 or P14 by injection of 3% carrageenan. We first determined paw edema in these mice to ensure that a similar degree of inflammation was achieved between the different age groups. At P7, the average paw volume ratio between the ipsilateral and contralateral hindpaws after carrageenan injection was 2.0 ± 0.1 at 1d, 1.6 ± 0.1 at 3d and 1.3 ± 0.1 at 7d post inflammation.
P7 Inflamed A-fibers
P7 Inflamed C-fibers
In contrast to results obtained from A-fiber neurons, no changes in mechanical (Naïve: 21.7 mN ± 14.2 mN; 1d: 20.3 mN ± 15.1 mN; 3d: 33.8 mN ± 22.3 mN; 7d: 28.6 mN ± 18.3 mN; p value < 0.96) or heat (Naïve: 47.2°C ± 2.5°C; 1d: 41.4°C ± 2.4°C; 3d: 41.0°C ± 1.1°C; 7d: 43.4°C ± 1.1°C; p value < 0.16) thresholds, or firing (Mechanical: Naïve: 52.2 Hz ± 27.7 Hz; 1d: 55.0 Hz ± 15.5 Hz; 3d: 43.3 Hz ± 11.5 Hz; 7d: 28.5 Hz ± 5.4 Hz; p value < 0.24; Heat: Naïve: 5.0 Hz ± 2.7 Hz; 1d: 10.1 Hz ± 4.6 Hz; 3d: 7.0 Hz ± 2.2 Hz; 7d: 9.4 Hz ± 2.3 Hz; p value < 0.74) to these peripheral stimuli were observed in the CPM neurons (Mechanical responses: Naive: n = 3; 1d: n = 6; 3d: n = 10; 7d: n = 19; Heat responses: Naive: n = 3; 1d: n = 7; 3d: n = 12; 7d: n = 15) at any time point after P7 inflammation of the hairy skin.
We also did not observe a statistically significant change in mechanical thresholds (Naïve: 21.0 mN ± 9.7 mN; 1d: 34.5 mN ± 13.5 mN; 3d: 66.0 mN ± 8.7 mN; 7d: 38.8 mN ± 17.0 mN; p value < 0.1) in the CM fibers (Naive: n = 11; 1d: n = 6; 3d: n = 12; 7d: n = 9) after P7 inflammation. Relative to naive mice (74.6 Hz ± 8.0 Hz), although no differences were found one day after carrageenan injection (97.4 Hz ± 12.0 Hz), we did find three (27.5 Hz ± 7.6 Hz) and seven (34.9 Hz ± 11.3 Hz) days after inflammation that CM fibers had lower firing to mechanical stimulation of the hairy skin (p value < 0.05). However, this is no different than the previously observed reduction in firing in these afferents during normal development using age-matched comparisons (see above). There was a statistically significant increase in mechanical firing one day after inflammation however, only when comparing results to time matched naives at P8 (Naïve: 50.6 Hz ± 9.8 Hz (n = 15); 1d inflamed: 97.4 Hz ± 12.0 Hz; p value < 0.05). Finally, we also found no differences in the response properties of CH neurons at any time point after P7 inflammation; however, low sampling prevents us from making firm statements about this result since few CH fibers were recorded and insufficient physiological data was obtained from this subtype at the various time points post P7 inflammation (not shown).
P14 Inflamed A-fibers
To then determine if a different pattern of afferent sensitization occurred after inflammation in the more developed animals, we performed ex vivo recording in mice with hairy skin inflammation initiated at P14. Paw edema was also consistent to that observed during P7 inflammation (above). At P14 the paw volume ratio between ipsilateral and contralateral hindpaws was 1.7 ± 0.1 at 1d, 1.5 ± 0.1 at 3d and 1.5 ± 0.1 at 7d. Interestingly, we found no differences in the response properties of the AM/APM neurons at any time point after P14 inflammation. No changes in mechanical thresholds (Naïve: 38.3 mN ± 30.9 mN; 1d: 11.3 mN ± 6.0 mN; 3d: 29.0 mN ± 13.3 mN; 60.0 mN ± 18.6 mN; p value < 0.19) or firing (Naïve: 75.4 Hz ± 69.6 Hz; 1d: 94.5 Hz ± 19.7 Hz; 3d: 76.1 Hz ± 14.5 Hz; 7d: 58.4 Hz ± 16.5 Hz; p value < 0.8) to mechanical deformation of the skin were detected (Naive: n = 3; 1d: n = 6; 3d: n = 8; 7d: n = 5). We also observed no changes in heat thresholds (Naïve: 42.6°C ± 5.3°C; 1d: 43.1°C ± 2.7°C; 3d: 39.9°C ± 3.1°C; 7d: 46.8°C ± 0°C; p value < 0.76), or firing (Naïve: 1.5 Hz ± 1.3 Hz; 1d: 0.6 Hz ± 0.4 Hz; 3d: 17.9Hz ± 13.7Hz; 7d: 1.3 Hz ± 0 Hz; p value < 0.63) to heat stimuli in these afferents subtypes (Naive: n = 2; 1d: n = 2; 3d: n = 3; 7d: n = 1).
These same results were obtained if we compared this data to the combined naïve AM/APMs from ages ≥ P14 (not shown). However, since we acquired a small number of heat sensitive afferents after P14 inflammation at the various time points, we also combined all data obtained from mice with cutaneous inflammation induced at P14. We again found that no differences were observed in heat thresholds (43.4°C ± 2.3°C, n = 4 vs 42.1°C ± 1.9°C, n = 6; p value < 0.68) or firing to heat stimuli (4.0 Hz ± 2.7 Hz, n = 3 vs. 9.3 Hz ± 7.2 Hz, n = 6; p value < 0.63) after P14 inflammation.
P14 Inflamed C-fibers
Age-specific changes in the prevalence of cutaneous afferents during neonatal inflammation
In addition, when we analyzed AM/APM fiber numbers, although no statistical differences were found in the numbers of AM fibers 1–7 days after inflammation induced at P7 (Naïve: 28%, n = 5/18; 1d: 44%, n = 11/25; 3d: 57%, n = 8/14; 7d: 36%, n = 5/14; p value < 0.38), we did observe a transient increase (36%, n = 9/25; p value < 0.044) in the numbers of the heat sensitive APM neurons one day after inflammation relative to P7 naives (17%, n = 3/18) that resolved by day three (13%, n = 2/14) and was maintained at day seven (21%, n = 3/14; not shown). However, unlike after P7 inflammation, P14 inflammation did not alter the percent of total fibers analyzed at any time point tested (AM: Naïve: 23%, n = 3/13; 1d: 21%, n = 3/14; 3d: 36%, n = 5/14; 7d: 50%, n = 3/6; p value < 0.54; APM: Naïve: 15%, n = 2/13; 1d: 21%, n = 3/14; 3d: 29%, n = 4/14; 7d: 33%, n = 2/6; p value < 0.79; CPM: Naïve: 47%, n = 18/38; 1d: 59%, n = 27/46; 3d: 56%, n = 20/36; 7d: 46%, n = 22/48; p value < 0.56; CM: Naïve: 32%, n = 12/38; 1d: 24%, n = 11/46; 3d: 31%, n = 11/36; 7d: 21%, n = 10/48; p value < 0.97; CH: Naïve: 11%, n = 4/38; 1d: 4%, n = 2/46; 3d: 3%, n = 1/36; 7d: 13%, n = 6/48; p value < 0.27).
Neurochemical identities of cutaneous afferents during neonatal inflammation
Naïve sensory fibers
Immunostaining results from intracellularly stained sensory neurons recovered from the ex vivo preparation
Postnatal Day 7/8
Postnatal Day 10≤
After P10, the one CM fiber that was neurochemically characterized still was not found to bind IB4 or stain positively for TRPV1. Although, the CPM fibers were often (4 of 8) found to bind IB4, they still were not immunoreactive for TRPV1 (Figure 5) at this age (≥P10), consistent with results obtained from adult mice [32, 33, 38]. In addition, we intracellularly filled one APM fiber at P7, two mechano-cold (MC) fibers (one with “A”-fiber CVs and one with “C”-fiber CVs) at P10 and one CH neuron at P14. The APM was found to be immunopositive for both TRPV1 and ASIC3 (Figure 5). The CH neuron was found to be TRPV1 positive and IB4 negative, and the two MC fibers were both found to be immunonegative for TRPV1 and did not bind IB4 (not shown) similar to adult animals (e.g. [32, 33, 38]).
Inflamed sensory fibers
After P14 inflammation, results were similar to P7 inflammation for the CPM neurons as some afferents (3 of 7) were found to be immunoreactive for TRPV1, unlike what was found in naïve mice (Figures 5, 6; Table 2). CPM neurons were still found to bind IB4 after P14 inflammation; however, fewer cells (1 of 7) were found to be positive for this marker compared to naives. This same IB4 positive CPM was also found to be immunopositive for TRPV1 (Figure 6I-L). Interestingly, the one CM fiber that was intracellularly filled with neurobiotin and immunocytochemically processed after P14 inflammation was found to be IB4 negative, but TRPV1 positive (Figure 6A-D; Table 2). However, the TRPV1 staining appeared to be intracellular and not at the cell membrane in this CM fiber. Additionally, we stained one APM fiber and one CH fiber after P14 inflammation. Both of these cells were found to be TRPV1 immunoreactive but not IB4 binding (not shown). In summary, IB4 binding appears to be detected in specific sensory neurons subtypes (CPM) after P10, but inflammation either before or after this time point may potentially promote TRPV1 staining in these cell types.
Cutaneous inflammation age-dependently alters gene expression in the DRGs
Percent changes in mRNA expression in the L2/L3 DRGs after inflammation
P7 Inflammation (1d)
P7 Inflammation (3d)
P14 Inflammation (1d)
P14 Inflammation (3d)
-46 ± 22#
-99 ± 30*
-46 ± 17
-92 ± 20*
-29 ± 27
-69 ± 35*
-1 ± 9
18 ± 10
-47 ± 17*
-99 ± 9*
-32 ± 12
-91 ± 11*
98 ± 36*
754 ± 34*
169 ± 19*
831 ± 31*
257 ± 31*
268 ± 15*
50 ± 22
387 ± 12*
18 ± 25
-15 ± 31
-18 ± 45
-11 ± 9
115 ± 24*
16 ± 34
-19 ± 60
-28 ± 7
143 ± 37*
-38 ± 14
83 ± 29*
-7 ± 12
-25 ± 23
-30 ± 7
84 ± 29*
-57 ± 39
52 ± 11*
-30 ± 20
4 ± 18
-36 ± 22
174 ± 33*
-20 ± 22
159 ± 29*
-47 ± 51
1 ± 28
52 ± 35
69 ± 10*
10 ± 76
107 ± 22*
-23 ± 20
-31 ± 27
-50 ± 40
-16 ± 15
40 ± 26
29 ± 15
21 ± 5
-14 ± 18
60 ± 20
58 ± 11*
87 ± 9*
Although the irritant receptor TRPA1 was found to be upregulated 1d after both P7 (143 ± 37%; p value < 0.05) and P14 (83 ± 29%; p value < 0.05) inflammation, as was the Mg2+ channel, TRPM6 (P7: 174 ± 33%; P14: 159 ± 29%; p values < 0.05), most receptors/channels that have been classically linked to sensory transduction were differentially altered in the DRGs after inflammation at P7 compared to P14. We found after P7 inflammation that ASIC3 (a receptor linked to pH sensitivity and mechanotransduction) was upregulated 1d after peripheral injury (115 ± 24%; p value < 0.05). In addition, we also detected a significant upregulation of the heat channel TRPM3 (52 ± 11%; p value < 0.05) and the heat threshold modulator and purinergic receptor, P2Y1 (107 ± 22%; p value < 0.05) 1d after P7 inflammation. Conversely, after P14 inflammation, we observed a unique set of changes in gene expression compared to P7. Specifically, we found a significant upregulation of the excitability regulator TRPC3 (84 ± 29%; p value < 0.05) and the heat transducing channel TRPV1 (69 ± 10%; p value < 0.05) 1d after P14 inflammation of the hairy skin. In addition, we also found that the mechanotransducer Piezo2 was upregulated in the DRGs after P14 inflammation at both 1d (58 ± 11%; p value < 0.05) and 3d (87 ± 9%; p value < 0.05). No changes in expression were observed in ASIC1 or P2X3 after inflammation at either P7 or P14 (Table 3).
Gene expression and sensory neuron development
It is a known phenomenon that specific sensory neurons that express trkA will down-regulate this receptor postnatally and increase expression of the ret tyrosine kinase receptor [22, 24]. This changes the sensitivity of these sensory neurons from NGF to GDNF, which regulates their proper development. We have found that at the same time trkA is downregulated and the ret receptor and GDNF co-receptor GFRα1 is upregulated (Table 1; Figure 7), the prevalence of CM neurons is decreased with a corresponding increase in the CPMs (Figure 1). This may suggest that these mechanically sensitive afferents are gaining heat responsiveness at the time they become responsive to GDNF. In support of this notion, mice that overexpress GDNF in the skin have significantly more heat responsive C-fibers . Thus the observed (Table 1, Figure 7) increase in GFRα1 expression in the DRGs may not only serve to support the normal maturation of sensory neurons , but also may play a role in the functional switch that occurs at the same time. In addition, mice that overexpress NGF in the skin also display increased numbers of heat sensitive C-fibers . NGF is thought to regulate afferent function (e.g. [26–28]) and GDNF dependent maturation of sensory neurons . Thus it would also be interesting to determine how NGF itself alters CM and CPM fiber frequency during this critical period in future experiments.
Changes in other receptors/channels in the DRGs may also play a role in altered function of individual subtypes. We observed a reduction in mechanical responsiveness in the CM population between P8 and P10. This altered response property correlated with our observed decrease in excitability regulator TRPC3 (; Table 1). Thus alterations in functional phenotype may be driven by changes in neurotrophic factor responsiveness while the limited changes in response properties in individual afferents are likely due to changes in specific receptors/channels associated with sensory transduction.
Age-dependent sensitization of cutaneous sensory neurons during inflammation
Although individual afferent response properties do not generally change during development ([19, 21, 30, 44]; current report), we and others  have shown that the prevalence of afferent subtypes does change. Myelinated afferents appear to lose heat sensitivity during the first week of life , while “C”-fibers may gain heat sensitivity during the second week (Figure 1). Thus if peripheral injury is experienced at the appropriate time, unique changes in primary afferents could generate neonatal pain in an age-related manner.
Here we examined whether peripheral inflammation immediately prior to our observed functional switch in afferent prevalence (P7) would produce a different set of changes in peripheral response properties compared to inflammation after the switch (P14). We found that the “A”-fiber nociceptors showed reduced thresholds to heat stimuli in addition to enhanced firing to both mechanical and heat stimulation of the skin after P7 inflammation (Figure 2). Widespread changes in afferent response properties were not observed in the “C”-fibers (not shown). This is different to that observed during P14 inflammation where the plasticity that is observed in sensory neurons occurs specifically in the “C”-fibers and not the “A”-fibers. Both CM and CPM neurons at this time showed increased responsiveness to mechanical stimuli and the CPM neurons also displayed enhanced heat responses (Figure 3). Thus even though individual afferents appear to be fully mature in terms of their peripheral response properties from as early as P0 in naïve animals (e.g. [19, 21, 30]), how these developing afferents respond to injury appears to be quite different depending on the stage of development that injury is sustained (Figure 8). Therefore, acute pain may be generated in neonates (e.g. [6, 40, 41]) through these unique types of alterations in afferents.
The mechanisms of how these changes may occur in sensory neurons after developmental inflammation may also be due to unique changes in gene expression in the DRGs since reports in adult animals have shown that dynamic changes in DRG gene expression are critically linked to altered sensory function after peripheral injuries (e.g. [38, 45, 46]). After P7 inflammation, we found a unique increase in the pH sensor and mechanical modulator ASIC3 [47, 48], the heat transducer TRPM3  and the heat threshold regulator P2Y1 [45, 50] in the DRGs (Table 3). These specific changes in gene expression correlate well with the observed changes in mechanical and heat responsiveness in addition to the reduction in heat thresholds in “A”-fiber neurons, respectively (Figure 2). Conversely, after P14 inflammation, we do not observe changes in these receptors, but we do observe an increase in the excitability regulator TRPC3 , the mechanotransducer piezo2 [51, 52] and the heat channel TRPV1 [53, 54] in the DRGs (Table 3). This correlates with the observed changes in mechanical responsiveness in the CPM and CM neurons and the enhanced responses to heat stimuli in the CPMs, respectively (Figure 3). Thus, not only are there unique subsets of afferents that sensitize after developmental inflammation depending upon the age at which injury is induced, but the changes in receptors/channels that may be mediating the altered peripheral responsiveness may also be age-dependent (Figure 8). Of course, we recognize the limitations of whole DRG analyses and acknowledge that a specific analysis of individual afferent function after developmental inflammation in the context of particular receptor inhibition will be necessary to clearly determine mechanisms.
Additionally, we observed changes in neurochemical identity in specific afferent types irrespective of postnatal age. Similar to previous reports in adult mice after peripheral injury [32, 45, 55] we found CPM neurons that expressed the TRPV1 channel after inflammation at both P7 and P14, which is not normally observed in naïve CPMs (Table 2; Figures 5, 6). If a similar phenomenon occurs in neonates as in adults, then the TRPV1 immunopositive CH neurons (not shown) may be gaining mechanical sensitivity, placing them in the CPM subcategory [32, 45]. This was thought to be due to the fact that peripheral injury can upregulate IB4 binding in sensory neurons in adults . In support of this idea in neonates, peripheral injuries can also induce IB4 staining in DRGs during early development . However, a more detailed analysis of the neurochemical identities of single sensory neurons is necessary to confirm this notion.
Treatment for pain in children is often similar to that of adults; however, side effects from pharmacological agents such as opioids can be more harmful in children [56–58]. Thus development of more suitable pharmacotherapies for pediatric pain has been of great interest. To this end, key research in the developing spinal cord has been performed to determine how noxious information from the periphery is processed leading to altered acute and long-term pain perception (e.g. [9, 59–64]). A central finding of these studies is that early neonatal injury accelerates dorsal horn circuit development likely via altered “A”-fiber inputs and erroneous “C”-fiber regulation of developing glycerinergic inhibition [64–68]. Our data presented here supports this finding in that we show enhanced responsiveness of the “A”-fiber nociceptors to mechanical and thermal stimuli after P7 inflammation (Figure 2) and a delay in the normal development of CM and CPM neurons (Figure 4). This latter finding may be due to reduced GFRα1 expression in the DRGs at this time after inflammation (Figure 7; Tables 1 and 3). Together, this may be a reason why early neonatal injury specifically causes long-term alterations of nociception and physiological function compared to peripheral injuries that occur later in life [9, 59, 60, 62, 63]. Peripheral injuries during this early time point in development appear to be altering sensory function which in turn incorrectly facilitates the development of spinal dorsal horn circuits and this may lead to subsequent alterations in supraspinal pathways and thus long-term alterations in nociception. Peripheral injury after the observed critical period of sensory development when peripheral fibers have fully matured could be one reason there are not long term affects seen when injuries are sustained later in development. This is a potentially important consideration for newborn infants or pre-term babies that undergo painful procedures or experience peripheral injuries [69, 70].
Therefore a major consideration for pediatric pain management is the age in which peripheral injury is sustained. We have shown that the plasticity that occurs in individual sensory neurons and the upregulation of specific receptors/channels in the DRGs that may be mediating the changes in afferents is age-dependent during development and different than that observed in adults ([1, 32, 45]; current report). Thus the animal’s age, the afferents potentially mediating the pain and the receptors within those afferents are critical factors when formulating proper pain therapies for neonates.
Swiss Webster mice from postnatal day 0 through postnatal day 21 were used in these studies. All animals were housed with the mother, which was provided food and water ad libitum and maintained on a 12 hr light/dark cycle. All procedures were approved by the Institutional Animal Care and Use Committee at Cincinnati Children’s Hospital Medical Center, under AAALAC approved practices.
Injection of carrageenan
All mice were anesthetized under 3% isofluorane during cutaneous injections. Using a syringe with a 30 g needle, 3-14 μL of 3% carrageenan (in 0.9% NaCl) was injected into the right hairy hindpaw skin. We used 1 μL/g body weight as a guide for these injections . Measurements of paw edema (paw volume) using calipers on the ipsilateral hindpaw relative to the contralateral hindpaw at 1–7 days after injection ensured that a relatively similar degree of inflammation was achieved and maintained at the various developmental time points. Although this methodology does not specifically allow for conclusions to be drawn about the inflammatory process itself or the developmental regulation of peripheral inflammation, it does serve as a general guide to ensure we were not differentially inflaming the skin of mice at different ages/weights. Injections began at the ankle and the needle was slowly retracted as the agent was expelled from the syringe. A cotton swab was used after injections to prevent leakage of carrageenan. All electrophysiological, neurochemical or molecular analyses were performed 1d, 3d and 7d after injection of carrageenan and compared to naïve mice between P0 and P21 or with each other.
Ex vivo recording
The ex-vivo somatosensory system preparation has been described in detail previously for adult mice [32, 33]. We used a similar version of this same preparation in the current study in neonates. Briefly, male mice were anesthetized via injection of ketamine and xylazine (90 and 10 mg/kg, respectively) and perfused transcardially with oxygenated (95% O2-5% CO2) artificial CSF (aCSF; in mM: 1.9 KCl, 1.2 KH2PO4, 1.3 MgSO4, 2.4 CaCl2, 26.0 NaHCO3, and 10.0 D-glucose) containing 253.9 mM sucrose at approximately 12°C. The spinal cord and the right hindlimb was excised and placed in a bath of this aCSF. Hairy skin of the right hindpaw, saphenous nerve, L1-L5 DRGs and spinal cord were isolated. Following dissection, the preparation was transferred to a separate recording chamber containing chilled and oxygenated aCSF in which the sucrose was replaced with 127.0 mM NaCl. The skin was then pinned out on a stainless steel grid located at the bath/air interface, such that the dermal surface was allowed to be continuously perfused with the aCSF while the epidermis remained dry. The bath was then slowly warmed to 31°C before recording.
Recording and stimulation
Sensory neuron somata within the L2 or L3 DRGs were impaled with quartz microelectrodes (impedance >150 MΩ) containing 5% Neurobiotin (Vector Laboratories, Burlingame, CA) in 1 M potassium acetate. Orthograde electrical search stimuli were delivered through a suction electrode placed on the side of the nerve to locate sensory neuron somata innervating the skin. Cutaneous receptive fields (RF) were localized with a soft brush and/or von Frey filaments. When cells were driven by the nerve but had no mechanical RF, a thermal search was conducted by applying hot (~52°C) and/or cold (~1°C) physiological saline to the skin. Although a concern may arise from this type of stimulation where brief but multiple applications of hot saline would result in sensitization of nociceptors during the course of an experiment, this has been thoroughly examined previously and has shown to not produce sensitization of other fibers recorded in the same preparations. That is to say, no differences were observed between fibers recorded at the beginning of an experiment compared to the end (e.g. [32, 33, 38]). Similar results were found here.
The response characteristics of individual DRG cells were then determined by applying mechanical and thermal stimuli to the hairy skin. For mechanical stimulation, RFs were probed with an increasing series of calibrated von Frey (VF) filaments ranging from 0.07 g to 10 g. When feasible, a mechanical stimulator that delivered a digitally controlled mechanical stimulus was also employed, which consisted of a tension/length controller (Aurora Scientific) attached to a probe with a 1 mm diameter aluminum tip. Computer controlled 5 s square waves of 1, 5, 10, 25, 50 and 100 mN were applied to the cell’s RF. In order to compare these results to those of the VF stimulation, VF units in grams were converted to a mN force based on the VF diameter. After mechanical stimulation, a controlled thermal stimulus was applied using a 3 mm2 contact area peltier element (Yale Univ. Machine Shop). Cold stimuli consisted of a variable rate cold ramp beginning at 31°C and reaching approximately 2-4°C, held for approximately 4-5 s and slowly allowed to return to 31°C. Bath temperature was maintained for a brief period and then the heat stimulus was initiated, which consisted of a 12 s heat ramp from 31-52°C followed by a 5 s plateau at 52°C. The stimulus then ramped back down to 31°C in 12 s. Adequate recovery times (approx. 20-30 s) were employed between stimulations. When recording from some myelinated sensory neurons, the heat ramp was continued to 54°C rather than 52°C and held for 5 s. In other instances, when fibers were unable to be fully characterized by controlled mechanical and thermal stimulation but were partially characterized by one of the controlled stimuli and brush or saline stimuli, these cells were also included for determination of afferent subtype prevalence and for the properties in which we obtained controlled data. All responses were recorded for offline analysis (Spike2 software, Cambridge Electronic Design). After physiological characterization, select cells were iontophoretically injected with 5% Neurobiotin (up to 2 cells per DRG). Conduction velocities of the recorded afferents were then calculated from spike latency and the distance between stimulating and recording electrodes (measured directly along the nerve). A total of 602 cells were intracellularly recorded and physiologically characterized in the current study. The average number of cells recorded per condition/time point/age was 54, which were obtained from an average of four mice per preparation. The minimum number of mice per preparation was three and an average of approximately 14 cells was recorded from each preparation. A minimum of 50 cells were obtained from each of the 11 groups analyzed. Specifically, the numbers of animals and cells recorded per condition were as follows: Naïve conditions: P7: 4 mice, 50 cells; P8: 6 mice, 54 cells; P10: 5 mice, 53 cells; P14: 5 mice, 58 cells; P21: 4 mice, 57 cells. Inflamed conditions: P7 inflammation: 1d: 4 mice, 57 cells; 3d: 3 mice, 52 cells; 7d: 4 mice, 56 cells; P14 inflammation: 1d: 3 mice 61 cells, 3d: 3 mice, 50 cells; 7d: 3 mice, 54 cells.
Immunocytochemistry and analysis of single cells
After electrophysiological characterization and intracellular filling with Neurobiotin, the DRG containing the injected cell was removed and immersion fixed with 3% paraformaldehyde in 0.1 M phosphate buffer (PB) for 30 min at room temperature. Ganglia were then rinsed in 0.1 M PB, embedded in 10% gelatin, postfixed in 3% paraformaldehyde, and cryoprotected in 20% sucrose. 60 μm frozen sections were collected in PB from a sliding microtome (Thermo) and reacted with fluorescently-tagged (FITC) avidin to label Neurobiotin-filled cells (Vector Laboratories). Each section was also processed for ASIC3 (1:2000; Millipore; Cat#: AB5927; RRID: AB_92140) or TRPV1 (1:2000; Alomone; Cat# ACC-030; RRID: AB_2040256) immunoreactivity and isolectin B4 (IB4) binding (AlexaFluor 647; Molecular Probes). After incubation in primary antiserum, tissue was washed and incubated in appropriate fluorescently tagged secondary antibodies (1:200; Jackson Immunoresearch). Distribution of fluorescent staining was determined using Leica confocal microscope. Sequential scanning was performed to prevent bleed-through of the different fluorophores. Images were then captured and compiled using Adobe Photoshop.
RNA isolation, reverse transcription and realtime PCR
Animals were first anesthetized as described above. The mice were then intracardially perfused with ice cold 0.9% NaCl prior to dissection of DRGs. RNA isolation from the L2 and L3 DRGs was performed using Qiagen RNeasy mini kits for animal tissues using the supplied protocol (n = 3-5 per condition/time point). RNA concentrations were then determined by obtaining A260 readings on a Nanodrop spectrometer (Thermo). Purified RNA was treated with DNase I (Invitrogen) and then DNased RNA was reverse transcribed using Superscript II Reverse Transcriptase (Invitrogen). For realtime PCR, 20 ng samples of cDNA were added to a SYBR Green Master Mix (Applied Biosystems) containing the appropriate primer combinations and run in duplicate on an Applied Biosystems Imager. Forward and reverse primer sequences used in realtime PCR reactions for ASIC1, ASIC3, GFRα3, TRPV1, TRPA1, trkA and GAPDH were obtained from Elitt et al. . GFRα1, P2Y1 and P2X3 primer sequences were obtained from Jankowski et al. . Primer sequences used for TRPC3, TRPM3, TRPM6, Piezo2, IGFr1 and IL1r1 are as follows: TRPC3: forward: 5′-AAG CAG GAT ATC TCC AGC CTT CGT-3′; reverse: 5′-AAG ATG GCT AAT TCC TCC GTC GCT-3′; TRPM3: forward: 5′-TTG AGG GAC CAG CTG TTG GT-3′; reverse: 5′-GTG CTG AGC TTG GGT TCG A-3′; TRPM6: forward: 5′-GTC TAC TGC CAT TCA GCC AAC CAA-3′; reverse: 5′-AGC CAA CAT CAG TTC TTC CAG GGT-3′; Piezo2: forward: 5′-AAG CCT TGG AAC TGG TGG TCT TCA-3′; reverse: 5′-ATA CCA TAG CCA GCC AAG AAG CCT-3′; IGFr1: forward: 5′-TTG AAC TTA TGC GCA TGT GCT GGC-3′; reverse: 5′-TCT CAT CCT TGA TGC TGC CGA TGA-3′; IL1r1: forward: 5′-AGG AAT GTG GCT GAA GAG CAC AGA-3′; reverse: 5′-ACT CGT GTG ACC GGA TAT TGC TTC-3′. Cycle time (Ct) values were normalized to GAPDH and changes in expression are calculated as a ΔΔCt value that is determined by subtracting the Ct values of the gene of interest from the GAPDH internal control for each sample and compared among samples. Fold change is described as 2ΔΔCt (Applied Biosystems) and 2-fold change equals 100% change (mean ± SEM).
Protein isolation and western blotting
Pooled L2/L3 DRGs from two naïve or peripherally inflamed mice were homogenized in lysis buffer containing 1% sodium dodecyl sulfate (SDS), 10 mM Tris–HCl (pH 7.4), and protease inhibitors (1 μg/ml pepstatin, 1 μg/ml leupeptin, 1 μg/ml aprotinin, 1 mM sodium orthovanadate and 100 μg/ml phenylmethylsulfonyl fluoride; Sigma Biochemicals). Then three to four of these pooled samples (15 μg) from each condition were centrifuged and boiled 10 min in a denaturing buffer containing β-mercaptoethanol and SDS prior to gel electrophoresis. Samples were then separated on a 12% polyacrylamide SDS-PAGE gel and transferred to a PVDF (Millipore) membrane that was blocked in specialized LiCor blocking buffer. Membranes were then incubated in primary antibodies overnight at 4°C (GFRα1: 1:250; R&D; Cat#: AF560; RRID: AB_2110307; GAPDH: 1:1000; ProSci Inc.; Cat#: XW-7214; RRID: AB_735758). Antibody binding was visualized using 680 nm or 800 nm infrared dye conjugated donkey anti-goat (Cat# 926–32214; RRID: AB_621846), or donkey anti-chicken (Cat# 926–32228; RRID: AB_1850018) secondary antibodies (1:15,000; LiCor) with detection using the LiCor Odyssey Imaging System (LiCor). Settings for detection were consistent between runs. Immunoreactive bands were analyzed by densitometry and quantified using NIH image J (RRID: nif-0000-30467) software. Band intensity was normalized to GAPDH and reported as a percent change (mean ± SEM).
For electrophysiological analyses, one-way ANOVA tests and posthoc analysis (Tukey) or non-parametric, Kruskal-Wallis tests and posthoc analysis (Dunn’s test) were used to analyze data associated with mechanical and thermal thresholds and mean peak instantaneous frequencies (mean ± SEM) of both fast conducting and slowly conducting fibers [38, 72]. This information was sorted by modality to examine whether certain subpopulations of sensory neurons have any consistency in regards to their response properties or expression of any of the markers used. In order to determine changes in the percentage of total fibers recorded among the multiple groups analyzed, a χ2 test was employed. In the few cases in which low numbers of afferents were obtained for a particular subpopulation, data were compared across conditions by also combining data from specific age groups to ascertain a generalized idea of the changes in response properties of afferents recorded during development or after inflammation. For a few ages, we obtained low numbers for only the mechanically sensitive A-fiber afferents that also responded to heat (APM), the small populations of mechanically insensitive but heat sensitive C-fibers (CH) and the polymodal C-fibers (CPM) that also responded to cold at certain ages/conditions (see Results above). Therefore in order to at least obtain a broad understanding of the potential changes in these afferent subtypes during the second and third weeks of development, data were combined for time points prior to P14 (P7-P10) and after P14 (P14-P21) and re-analyzed as described above. Combining data in this manner should allow us to determine general differences between the groups being analyzed as previous reports have shown that after approximately P7, no changes in response properties, particularly in A-fiber afferents, are typically detected in naïve animals [19, 21, 30]. Combining data in this manner was also performed from the time points post inflammation to more firmly state whether these particular subpopulations displayed altered response properties after peripheral injury. For mRNA or protein analyses, one-way ANOVA with Tukey’s post hoc assays were utilized. In order to determine the relative changes in gene expression that occur during postnatal development, all naïve time points were compared to L2/L3 DRGs obtained at P0 (n = 4). DRGs obtained after inflammation however, were compared to age-matched naïve controls. P-values were all set at p < 0.05.
This work was supported by grants to MPJ from the Rita Allen Foundation and American Pain Society, the Trustees of Cincinnati Children’s Hospital Medical Center and the National Institutes of Health (R03HD077483). We would also like to thank Dr. Mark Baccei for insightful comments on the manuscript.
- Jankowski MP, Koerber HR: Neurotrophic Factors and Nociceptor Sensitization. In Translational Pain Research: From Mouse to Man. Edited by: Kruger L, Light AR. Boca Raton, FL: CRC Press; 2010:31–50.Google Scholar
- Cadiou H, Studer M, Jones NG, Smith ES, Ballard A, McMahon SB, McNaughton PA: Modulation of acid-sensing ion channel activity by nitric oxide. J Neurosci 2007, 27: 13251–13260. 10.1523/JNEUROSCI.2135-07.2007PubMedView ArticleGoogle Scholar
- White FA, Sun J, Waters SM, Ma C, Ren D, Ripsch M, Steflik J, Cortright DN, Lamotte RH, Miller RJ: Excitatory monocyte chemoattractant protein-1 signaling is up-regulated in sensory neurons after chronic compression of the dorsal root ganglion. Proc Natl Acad Sci U S A 2005, 102: 14092–14097. 10.1073/pnas.0503496102PubMed CentralPubMedView ArticleGoogle Scholar
- Walker SM, Meredith-Middleton J, Cooke-Yarborough C, Fitzgerald M: Neonatal inflammation and primary afferent terminal plasticity in the rat dorsal horn. Pain 2003, 105: 185–195. 10.1016/S0304-3959(03)00201-XPubMedView ArticleGoogle Scholar
- Pollock J, McFarlane SM, Connell MC, Zehavi U, Vandenabeele P, MacEwan DJ, Scott RH: TNF-alpha receptors simultaneously activate Ca2+ mobilisation and stress kinases in cultured sensory neurones. Neuropharmacology 2002, 42: 93–106. 10.1016/S0028-3908(01)00163-0PubMedView ArticleGoogle Scholar
- Beland B, Fitzgerald M: Mu- and delta-opioid receptors are downregulated in the largest diameter primary sensory neurons during postnatal development in rats. Pain 2001, 90: 143–150. 10.1016/S0304-3959(00)00397-3PubMedView ArticleGoogle Scholar
- Ross RA, Coutts AA, McFarlane SM, Anavi-Goffer S, Irving AJ, Pertwee RG, MacEwan DJ, Scott RH: Actions of cannabinoid receptor ligands on rat cultured sensory neurones: implications for antinociception. Neuropharmacology 2001, 40: 221–232. 10.1016/S0028-3908(00)00135-0PubMedView ArticleGoogle Scholar
- Wood JN, Winter J, James IF, Rang HP, Yeats J, Bevan S: Capsaicin-induced ion fluxes in dorsal root ganglion cells in culture. J Neurosci 1988, 8: 3208–3220.PubMedGoogle Scholar
- Vega-Avelaira D, McKelvey R, Hathway G, Fitzgerald M: The emergence of adolescent onset pain hypersensitivity following neonatal nerve injury. Mol Pain 2012, 8: 30. 10.1186/1744-8069-8-30PubMed CentralPubMedView ArticleGoogle Scholar
- Vega-Avelaira D, Geranton SM, Fitzgerald M: Differential regulation of immune responses and macrophage/neuron interactions in the dorsal root ganglion in young and adult rats following nerve injury. Mol Pain 2009, 5: 70. 10.1186/1744-8069-5-70PubMed CentralPubMedView ArticleGoogle Scholar
- Bourane S, Mechaly I, Venteo S, Garces A, Fichard A, Valmier J, Carroll P: A SAGE-based screen for genes expressed in sub-populations of neurons in the mouse dorsal root ganglion. BMC Neurosci 2007, 8: 97. 10.1186/1471-2202-8-97PubMed CentralPubMedView ArticleGoogle Scholar
- Elg S, Marmigere F, Mattsson JP, Ernfors P: Cellular subtype distribution and developmental regulation of TRPC channel members in the mouse dorsal root ganglion. J Comp Neurol 2007, 503: 35–46. 10.1002/cne.21351PubMedView ArticleGoogle Scholar
- Beland B, Fitzgerald M: Influence of peripheral inflammation on the postnatal maturation of primary sensory neuron phenotype in rats. J Pain 2001, 2: 36–45. 10.1054/jpai.2001.17697PubMedView ArticleGoogle Scholar
- Lee SY, Hong Y, Oh U: Decreased pain sensitivity of capsaicin-treated rats results from decreased VR1 expression. Arch Pharm Res 2004, 27: 1154–1160. 10.1007/BF02975122PubMedView ArticleGoogle Scholar
- Nandi R, Beacham D, Middleton J, Koltzenburg M, Howard RF, Fitzgerald M: The functional expression of mu opioid receptors on sensory neurons is developmentally regulated; morphine analgesia is less selective in the neonate. Pain 2004, 111: 38–50. 10.1016/j.pain.2004.05.025PubMedView ArticleGoogle Scholar
- Boada MD, Houle TT, Eisenach JC, Ririe DG: Differing neurophysiologic mechanosensory input from glabrous and hairy skin in juvenile rats. J Neurophysiol 2010, 104: 3568–3575. 10.1152/jn.00415.2010PubMed CentralPubMedView ArticleGoogle Scholar
- Woodbury CJ, Koerber HR: Central and peripheral anatomy of slowly adapting type I low-threshold mechanoreceptors innervating trunk skin of neonatal mice. J Comp Neurol 2007, 505: 547–561. 10.1002/cne.21517PubMedView ArticleGoogle Scholar
- Woodbury CJ, Koerber HR: Widespread projections from myelinated nociceptors throughout the substantia gelatinosa provide novel insights into neonatal hypersensitivity. J Neurosci 2003, 23: 601–610.PubMedGoogle Scholar
- Koerber HR, Woodbury CJ: Comprehensive phenotyping of sensory neurons using an ex vivo somatosensory system. Physiol Behav 2002, 77: 589–594. 10.1016/S0031-9384(02)00904-6PubMedView ArticleGoogle Scholar
- Woodbury CJ, Ritter AM, Koerber HR: Central anatomy of individual rapidly adapting low-threshold mechanoreceptors innervating the “hairy” skin of newborn mice: early maturation of hair follicle afferents. J Comp Neurol 2001, 436: 304–323. 10.1002/cne.1069PubMedView ArticleGoogle Scholar
- Fitzgerald M: Cutaneous primary afferent properties in the hind limb of the neonatal rat. J Physiol 1987, 383: 79–92.PubMed CentralPubMedGoogle Scholar
- Luo W, Wickramasinghe SR, Savitt JM, Griffin JW, Dawson TM, Ginty DD: A hierarchical NGF signaling cascade controls Ret-dependent and Ret-independent events during development of nonpeptidergic DRG neurons. Neuron 2007, 54: 739–754. 10.1016/j.neuron.2007.04.027PubMedView ArticleGoogle Scholar
- Patapoutian A, Reichardt LF: Trk receptors: mediators of neurotrophin action. Curr Opin Neurobiol 2001, 11: 272–280. 10.1016/S0959-4388(00)00208-7PubMedView ArticleGoogle Scholar
- Molliver DC, Wright DE, Leitner ML, Parsadanian AS, Doster K, Wen D, Yan Q, Snider WD: IB4-binding DRG neurons switch from NGF to GDNF dependence in early postnatal life. Neuron 1997, 19: 849–861. 10.1016/S0896-6273(00)80966-6PubMedView ArticleGoogle Scholar
- Lewin GR, Ritter AM, Mendell LM: On the role of nerve growth factor in the development of myelinated nociceptors. J Neurosci 1992, 12: 1896–1905.PubMedGoogle Scholar
- Ritter AM, Lewin GR, Mendell LM: Regulation of myelinated nociceptor function by nerve growth factor in neonatal and adult rats. Brain Res Bull 1993, 30: 245–249. 10.1016/0361-9230(93)90251-6PubMedView ArticleGoogle Scholar
- Ritter AM, Lewin GR, Kremer NE, Mendell LM: Requirement for nerve growth factor in the development of myelinated nociceptors in vivo. Nature 1991, 350: 500–502. 10.1038/350500a0PubMedView ArticleGoogle Scholar
- Lewin GR, Mendell LM: Regulation of cutaneous C-fiber heat nociceptors by nerve growth factor in the developing rat. J Neurophysiol 1994, 71: 941–949.PubMedGoogle Scholar
- Albers KM, Woodbury CJ, Ritter AM, Davis BM, Koerber HR: Glial cell-line-derived neurotrophic factor expression in skin alters the mechanical sensitivity of cutaneous nociceptors. J Neurosci 2006, 26: 2981–2990. 10.1523/JNEUROSCI.4863-05.2006PubMedView ArticleGoogle Scholar
- Ye Y, Woodbury CJ: Early postnatal loss of heat sensitivity among cutaneous myelinated nociceptors in Swiss-Webster mice. J Neurophysiol 2010, 103: 1385–1396. 10.1152/jn.00472.2009PubMed CentralPubMedView ArticleGoogle Scholar
- Boada MD, Gutierrez S, Giffear K, Eisenach JC, Ririe DG: Skin incision-induced receptive field responses of mechanosensitive peripheral neurons are developmentally regulated in the rat. J Neurophysiol 2012, 108: 1122–1129. 10.1152/jn.00399.2012PubMed CentralPubMedView ArticleGoogle Scholar
- Jankowski MP, Lawson JJ, McIlwrath SL, Rau KK, Anderson CE, Albers KM, Koerber HR: Sensitization of cutaneous nociceptors after nerve transection and regeneration: possible role of target-derived neurotrophic factor signaling. J Neurosci 2009, 29: 1636–1647. 10.1523/JNEUROSCI.3474-08.2009PubMed CentralPubMedView ArticleGoogle Scholar
- Lawson JJ, McIlwrath SL, Woodbury CJ, Davis BM, Koerber HR: TRPV1 unlike TRPV2 is restricted to a subset of mechanically insensitive cutaneous nociceptors responding to heat. J Pain 2008, 9: 298–308. 10.1016/j.jpain.2007.12.001PubMed CentralPubMedView ArticleGoogle Scholar
- Staaf S, Oerther S, Luca G, Mattsson JP, Ernfors P: Differential regulation of TRP channels in a rat model of neuropathic pain. Pain 2009, 144: 187–199. 10.1016/j.pain.2009.04.013PubMedView ArticleGoogle Scholar
- Schlingmann KP, Weber S, Peters M, Niemann Nejsum L, Vitzthum H, Klingel K, Kratz M, Haddad E, Ristoff E, Dinour D, Syrrou M, Nielsen S, Sassen M, Waldegger S, Seyberth HW, Konrad M: Hypomagnesemia with secondary hypoglycemia is caused by mutations in TRPM6, a new member of the TRPM gene family. Nat Genet 2002, 31: 166–170. 10.1038/ng889PubMedView ArticleGoogle Scholar
- Babes A, Zorzon D, Reid G: Two populations of cold-sensitive neurons in rat dorsal root ganlgia and their modulation by nerve growth factor. Eur J Neurosci 2004, 20: 2276–2282. 10.1111/j.1460-9568.2004.03695.xPubMedView ArticleGoogle Scholar
- Story GM, Peier AM, Reeve AJ, Eid SR, Mosbacher J, Hricik TR, Earley TJ, Hergarden AC, Andersson DA, Hwang SW, McIntyre P, Jegla T, Bevan S, Patapoutian A: ANKTM1, a TRP-like channel expressed in nociceptive neurons, is activated by cold temperatures. Cell 2003, 112: 819–829. 10.1016/S0092-8674(03)00158-2PubMedView ArticleGoogle Scholar
- Jankowski MP, Rau KK, Soneji DJ, Anderson CE, Koerber HR: Enhanced artemin/GFRalpha3 levels regulate mechanically insensitive, heat-sensitive C-fiber recruitment after axotomy and regeneration. J Neurosci 2010, 30: 16272–16283. 10.1523/JNEUROSCI.2195-10.2010PubMed CentralPubMedView ArticleGoogle Scholar
- McIlwrath SL, Lawson JJ, Anderson CE, Albers KM, Koerber HR: Overexpression of neurotrophin-3 enhances the mechanical response properties of slowly adapting type 1 afferents and myelinated nociceptors. Eur J Neurosci 2007, 26: 1801–1812. 10.1111/j.1460-9568.2007.05821.xPubMedView ArticleGoogle Scholar
- Sweitzer SM, Allen CP, Zissen MH, Kendig JJ: Mechanical allodynia and thermal hyperalgesia upon acute opioid withdrawal in the neonatal rat. Pain 2004, 110: 269–280. 10.1016/j.pain.2004.04.003PubMedView ArticleGoogle Scholar
- Marsh D, Dickenson A, Hatch D, Fitzgerald M: Epidural opioid analgesia in infant rats II: responses to carrageenan and capsaicin. Pain 1999, 82: 33–38. 10.1016/S0304-3959(99)00029-9PubMedView ArticleGoogle Scholar
- Stucky CL, Koltzenberg M, Schneider M, Engle MG, Albers KM, Davis BM: Overexpression of nerve growth factor in skin selectively affects the survival and functional properties of nociceptors. J Neurosci 1999, 19: 8509–8516.PubMedGoogle Scholar
- Qu L, Li Y, Pan X, Zhang P, LaMotte RH, Ma C: Transient receptor potential canonical 3 (TRPC3) is required for IgG immune complex-induced excitation of the rat dorsal root ganglion neurons. J Neurosci 2012, 32: 9554–9562. 10.1523/JNEUROSCI.6355-11.2012PubMed CentralPubMedView ArticleGoogle Scholar
- Koltzenburg M, Stucky CL, Lewin GR: Receptive properties of mouse sensory neurons innervating hairy skin. J Neurophysiol 1997, 78: 1841–50.PubMedGoogle Scholar
- Jankowski MP, Rau KK, Soneji DJ, Ekmann KM, Anderson CE, Molliver DC, Koerber HR: Purinergic receptor P2Y1 regulates polymodal C-fiber thermal thresholds and sensory neuron phenotypic switching during peripheral inflammation. Pain 2012, 153: 410–419. 10.1016/j.pain.2011.10.042PubMed CentralPubMedView ArticleGoogle Scholar
- Jankowski MP, Soneji DJ, Ekmann KM, Anderson CE, Koerber HR: Dynamic changes in heat transducing channel TRPV1 expression regulate mechanically insensitive, heat sensitive C-fiber recruitment after axotomy and regeneration. J Neurosci 2012, 32: 17869–17873. 10.1523/JNEUROSCI.3148-12.2012PubMed CentralPubMedView ArticleGoogle Scholar
- Walder RY, Rasmussen LA, Rainier JD, Light AR, Wemmie JA, Sluka KA: ASIC1 and ASIC3 play different roles in the development of Hyperalgesia after inflammatory muscle injury. J Pain 2010, 11: 210–218. 10.1016/j.jpain.2009.07.004PubMed CentralPubMedView ArticleGoogle Scholar
- Sluka KA, Price MP, Breese NM, Stucky CL, Wemmie JA, Welsh MJ: Chronic hyperalgesia induced by repeated acid injections in muscle is abolished by the loss of ASIC3, but not ASIC1. Pain 2003, 106: 229–239. 10.1016/S0304-3959(03)00269-0PubMedView ArticleGoogle Scholar
- Vriens J, Owsianik G, Hofmann T, Philipp SE, Stab J, Chen X, Benoit M, Xue F, Janssens A, Kerselaers S, Oberwinkler J, Vennekens R, Gudermann T, Nilius B, Voets T: TRPM3 is a nociceptor channel involved in the detection of noxious heat. Neuron 2011, 70: 482–494. 10.1016/j.neuron.2011.02.051PubMedView ArticleGoogle Scholar
- Molliver DC, Rau KK, McIlwrath SL, Jankowski MP, Koerber HR: The ADP receptor P2Y1 is necessary for normal thermal sensitivity in cutaneous polymodal nociceptors. Mol Pain 2011, 7: 13. 10.1186/1744-8069-7-13PubMed CentralPubMedView ArticleGoogle Scholar
- Coste B, Xiao B, Santos JS, Syeda R, Grandl J, Spencer KS, Kim SE, Schmidt M, Mathur J, Dubin AE, Montal M, Patapoutian A: Piezo proteins are pore-forming subunits of mechanically activated channels. Nature 2012, 483: 176–181. 10.1038/nature10812PubMed CentralPubMedView ArticleGoogle Scholar
- Kim SE, Coste B, Chadha A, Cook B, Patapoutian A: The role of Drosophila Piezo in mechanical nociception. Nature 2012, 483: 209–212. 10.1038/nature10801PubMed CentralPubMedView ArticleGoogle Scholar
- Caterina MJ, Leffler A, Malmberg AB, Martin WJ, Trafton J, Petersen-Zeitz KR, Koltzenburg M, Basbaum AI, Julius D: Impaired nociception and pain sensation in mice lacking the capsaicin receptor. Science 2000, 288: 306–313. 10.1126/science.288.5464.306PubMedView ArticleGoogle Scholar
- Caterina MJ, Schumacher MA, Tominaga M, Rosen TA, Levine JD, Julius D: The capsaicin receptor: a heat-activated ion channel in the pain pathway. Nature 1997, 389: 816–824. 10.1038/39807PubMedView ArticleGoogle Scholar
- Tsuda M, Koizumi S, Kita A, Shigemoto Y, Ueno S, Inoue K: Mechanical allodynia caused by intraplantar injection of P2X receptor agonist in rats: involvement of heteromeric P2X2/3 receptor signaling in capsaicin-insensitive primary afferent neurons. J Neurosci 2000, 20: RC90.PubMedGoogle Scholar
- Sadhasivam S, Chidambaran V: Pharmacogenomics of opioids and perioperative pain management. Pharmacogenomics 2012, 13: 1719–1740. 10.2217/pgs.12.152PubMedView ArticleGoogle Scholar
- Bouwmeester NJ, Anderson BJ, Tibboel D, Holford NH: Developmental pharmacokinetics of morphine and its metabolites in neonates, infants and young children. Br J Anaesth 2004, 92: 208–217. 10.1093/bja/aeh042PubMedView ArticleGoogle Scholar
- Zeltzer LK, Anderson CT, Schechter NL: Pediatric pain: current status and new directions. Curr Probl Pediatr 1990, 20: 409–486.PubMedGoogle Scholar
- Li J, Baccei ML: Neonatal tissue injury reduces the intrinsic excitability of adult mouse superficial dorsal horn neurons. Neuroscience 2014, 256: 392–402.PubMed CentralPubMedView ArticleGoogle Scholar
- Li J, Blankenship ML, Baccei ML: Inward-rectifying potassium (Kir) channels regulate pacemaker activity in spinal nociceptive circuits during early life. J Neurosci 2013, 33: 3352–3362. 10.1523/JNEUROSCI.4365-12.2013PubMed CentralPubMedView ArticleGoogle Scholar
- Li J, Xie W, Zhang JM, Baccei ML: Peripheral nerve injury sensitizes neonatal dorsal horn neurons to tumor necrosis factor-alpha. Mol Pain 2009, 5: 10. 10.1186/1744-8069-5-10PubMed CentralPubMedView ArticleGoogle Scholar
- Beggs S, Currie G, Salter MW, Fitzgerald M, Walker SM: Priming of adult pain responses by neonatal pain experience: maintenance by central neuroimmune activity. Brain 2012, 135: 404–417. 10.1093/brain/awr288PubMed CentralPubMedView ArticleGoogle Scholar
- Laprairie JL, Murphy AZ: Neonatal injury alters adult pain sensitivity by increasing opioid tone in the periaqueductal gray. Front Behav Neurosci 2009, 3: 31.PubMed CentralPubMedView ArticleGoogle Scholar
- Torsney C, Fitzgerald M: Age-dependent effects of peripheral inflammation on the electrophysiological properties of neonatal rat dorsal horn neurons. J Neurophysiol 2002, 87: 1311–1317.PubMedGoogle Scholar
- Koch SC, Fitzgerald M: Activity-dependent development of tactile and nociceptive spinal cord circuits. Ann N Y Acad Sci 2013, 1279: 97–102. 10.1111/nyas.12033PubMedView ArticleGoogle Scholar
- Koch SC, Tochiki KK, Hirschberg S, Fitzgerald M: C-fiber activity-dependent maturation of glycinergic inhibition in the spinal dorsal horn of the postnatal rat. Proc Natl Acad Sci U S A 2012, 109: 12201–12206. 10.1073/pnas.1118960109PubMed CentralPubMedView ArticleGoogle Scholar
- Labrakakis C, Lorenzo LE, Bories C, Ribeiro-da-Silva A, De Koninck Y: Inhibitory coupling between inhibitory interneurons in the spinal cord dorsal horn. Mol Pain 2009, 5: 24. 10.1186/1744-8069-5-24PubMed CentralPubMedView ArticleGoogle Scholar
- Beggs S, Torsney C, Drew LJ, Fitzgerald M: The postnatal reorganization of primary afferent input and dorsal horn cell receptive fields in the rat spinal cord is an activity-dependent process. Eur J Neurosci 2002, 16: 1249–1258. 10.1046/j.1460-9568.2002.02185.xPubMedView ArticleGoogle Scholar
- Walker SM, Tochiki KK, Fitzgerald M: Hindpaw incision in early life increases the hyperalgesic response to repeat surgical injury: critical period and dependence on initial afferent activity. Pain 2009, 147: 99–106. 10.1016/j.pain.2009.08.017PubMedView ArticleGoogle Scholar
- Page GG: Are there long-term consequences of pain in newborn or very young infants? J Perinat Educ 2004, 13: 10–17.PubMed CentralPubMedView ArticleGoogle Scholar
- Elitt CM, McIlwrath SL, Lawson JJ, Malin SA, Molliver DC, Cornuet PK, Koerber HR, Davis BM, Albers KM: Artemin overexpression in skin enhances expression of TRPV1 and TRPA1 in cutaneous sensory neurons and leads to behavioral sensitivity to heat and cold. J Neurosci 2006, 26: 8578–8587. 10.1523/JNEUROSCI.2185-06.2006PubMedView ArticleGoogle Scholar
- Woodbury CJ, Zwick M, Wang S, Lawson JJ, Caterina MJ, Koltzenburg M, Albers KM, Koerber HR, Davis BM: Nociceptors lacking TRPV1 and TRPV2 have normal heat responses. J Neurosci 2004, 24: 6410–6415. 10.1523/JNEUROSCI.1421-04.2004PubMedView ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.