Figure 6

Intracellularly stained and physiologically characterized afferents in mice with inflammation recovered after ex vivo recording. A neurobiotin (NB) stained (A) “C”-fiber neuron (arrows) that was recovered from a mouse seven days after P14 cutaneous inflammation was found to be mechanically sensitive but thermally insensitive (D), and was found to contain TRPV1 protein (B; red) but did not bind isolectin B4 (IB4; C; blue). The TRPV1 staining in this particular neuron did not appear to be membrane bound as the labeling pattern for TRPV1 was within the staining pattern for NB (A). One polymodal (H) “C”-fiber (arrows) that was filled with NB (E) was found to contain TRPV1 (F; red) but did not bind IB4 (G; blue). This cell was recovered from a mouse with cutaneous inflammation induced at P14 (three day time point). Finally, another polymodal “C”-fiber (L; arrows) that was intracellularly filled with NB (I) was found to contain both TRPV1 (J; red) and bound IB4 (K; blue). This latter cell was obtained from a mouse with inflammation at P7 at the one day time point. Scale bar for images: 40 μm.