Figure 1

Experimental outline. L5-spinal nerve transection (SNT) was performed on twelve male Wistar rats. L5 DRG tissue was harvested 7 days after surgery and tissue from 4 animals was pooled for RNA extraction; naive tissue was used as control. Total RNA samples (n = 3 SNT, n = 3 naive) were divided into technical replicates used in parallel for microarray analysis (Affymetrix Rat Exon 1.0 ST arrays) and RNA-seq in the Illumina GAIIx platform, in both cases following poly(A) enrichment.