Figure 10

Lack of co-localisation of EGFP and either substance P or PPTB in glutamatergic boutons in the GRP-EGFP mouse. a Immunostaining with antibodies against EGFP (green), substance P (SP, red) and PPTB (blue) in a single optical section through lamina II from the L2 segment of one of the GRP-EGFP mice. b shows the same field scanned to reveal VGLUT2 (grey). c a merged image. Several VGLUT2-immunoreactive profiles that also stain for EGFP are visible, and 3 of these are indicated with arrows. These are not stained for either substance P or PPTB. Other VGLUT2 boutons that are stained with the substance P or PPTB antibodies are also present in this field, and some of these are marked with arrowheads or double arrowheads, respectively. Scale bar = 10 μm.