CFTR mediates noradrenaline-induced ATP efflux from DRG neurons
© Kanno and Nishizaki; licensee BioMed Central Ltd. 2011
Received: 11 August 2011
Accepted: 24 September 2011
Published: 24 September 2011
In our earlier study, noradrenaline (NA) stimulated ATP release from dorsal root ganglion (DRG) neurons as mediated via β3 adrenoceptors linked to Gs protein involving protein kinase A (PKA) activation, to cause allodynia. The present study was conducted to understand how ATP is released from DRG neurons. In an outside-out patch-clamp configuration from acutely dissociated rat DRG neurons, single-channel currents, sensitive to the P2X receptor inhibitor PPADS, were evoked by approaching the patch-electrode tip close to a neuron, indicating that ATP is released from DRG neurons, to activate P2X receptor. NA increased the frequency of the single-channel events, but such NA effect was not found for DRG neurons transfected with the siRNA to silence the cystic fibrosis transmembrane conductance regulator (CFTR) gene. In the immunocytochemical study using acutely dissociated rat DRG cells, CFTR was expressed in neurons alone, but not satellite cells, fibroblasts, or Schwann cells. It is concluded from these results that CFTR mediates NA-induced ATP efflux from DRG neurons as an ATP channel.
The dorsal root ganglion (DRG) is a relay point in pain tracks and a variety of receptors expressed in DRG neurons engage transmission and modulation of pain. ATP receptors, that include ionotropic P2X receptors and metabotropic P2Y receptors, serve as a pain mediator in the DRG [1–9]. A growing body of evidence has pointed to the role of P2X receptors in acute, inflammatory and neuropathic pain, P2X3 receptor in spontaneous pain and heat hyperalgesia, P2X2/3 receptor in acute mechanical allodynia, and P2Y2 receptor in transient receptor potential vanilloid receptor 1-mediated thermal hypersensitivity.
Noradrenaline (NA)/adrenoceptor also participates in the regulation of pain in the DRG. Adrenoceptors are divided into α1 receptors that include α1a, α1b and α1d subtypes, α2 receptors that include α2a, α2b, α2c and α2d subtypes, and β receptors that include β1, β2, β3 and β4 subtypes. Peripheral nerve injury causes sympathetic nerve sprouting into the DRG [10, 11]. Peripheral nerve axotomy induces hyperexcitation of DRG neurons via a sympathetic pathway, responsible for neuropathic pain such as hyperalgesia and allodynia . Sciatic nerve injury upregulates expression of mRNAs for α1b, α2a, α2b, and β2 adrenoceptors in DRG neurons, and NA potentiates slow type of ATP-evoked currents via α1 adrenoceptors, that are monitored from DRG neurons . Notably, NA stimulates ATP release from DRG neurons as mediated via β3 adrenoceptors linked to Gs protein involving protein kinase A (PKA) activation, to cause allodynia . DRG neurons form no synapse in the DRG, and therefore, ATP, to activate the receptors, is not released from presynaptic terminals. Then, the big question addressing is where ATP comes from in the DRG. The present study aimed at answering this question.
CFTR is an ABC cassette protein and functions as Cl- and ATP channels [15, 16]. Intriguingly, CFTR is activated by PKA phosphorylation of the regulatory domain [17, 18]. In our earlier study, NA-induced increase in the frequency of P2XR-NeSCCs was inhibited by the selective β3 adrenoceptor inhibitor SR59230A, knocking-down β3 adrenoceptors, or the PKA inhibitor H-89 , indicating the implication of β3 adrenoceptors linked to Gs protein involving PKA activation in the ATP efflux. Overall, NA appears to promote ATP efflux from DRG neurons in a CFTR-dependent manner as mediated via a β3 adrenoceptor/PKA signaling pathway.
In summary, the results of the present study provide evidence that CFTR mediates NA-induced ATP efflux from DRG neurons. This may establish a novel pathway for activation of ATP receptors under the control of NA/β3 adrenoceptor.
Materials and methods
All procedures have been approved by the Animal Care and Use Committee at Hyogo College of Medicine and were in compliance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals.
Preparation of DRG cells
The DRGs in the L4 to L6 segment were isolated from Wistar rats (male, 6 w) and incubated 0.3% (w/v) collagenase at 37°C for 30 min. Then, cells were mechanically dissociated and plated on poly-L-lysine-coated glass cover slips. Experiments were carried out within 12-24 h after preparation.
Single-channel current recording
Outside-out patches were made from acutely dissociated DRG neurons. Single-channel currents were monitored with an Axopatch-200A amplifier (Axon Instruments Inc., Sunnyvale, CA, USA) in a recording chamber continuously superfused with an extracellular solution [in mM; 135 NaCl, 5 KCl, 2.5 CaCl2, 1 MgCl2, 5 N-2-hydroxyethyl piperazine-N'-2-ethansulfonic acid (HEPES), and 10 glucose, pH 7.3] containing 6,7-dinitroquinoxaline-2,3-dione (DNQX)(20 μM) and DL-2-amino-5-phosphonopentanoic acid (DL-AP5)(100 μM) at a flow rate of 2 ml/min at 21-22°C. The patch electrode-filling solution was as follows: (in mM) 135 Cs-gluconate, 5 CsCl, 2 MgCl2, 5 ethyleneglycol-bis-(β-aminoethyl ether)-N,N,N',N'-tetraacetic acid, 5 HEPES, 5 MgATP, and 1 Na2GTP, pH 7.2. Currents were analyzed using a pClamp software (version 9.2, Axon Instruments Inc.). NA was bath-applied with an air-pressure injector (PicoPump PV 820, World Precision Instruments, Stevenage, UK).
Construction and transfection of siRNA
The CFTR siRNA was: 5'-GCUUAAAGGAAGAGGAUAUTT-3' and 5'-AUAUCCUCUUCCUUUAAGCTT-3' (B-Bridge International, Inc., Sunnyvale, CA, USA). The NC siRNA with the scrambled sequence, and the same GC content and nucleic acid composition was used as a negative control (B-Bridge International Inc.). Acutely dissociated neurons were transfected with the NC siRNA or the CFTR siRNA together with an enhanced green fluorescence protein (eGFP)-expression vector using a Nucleofector kit (Amaxa GmbH, Cologne, Germany). Single-channel recording and single-cell quantitative real-time RT-PCR were carried out 24 h after transfection.
Single-cell quantitative real-time RT-PCR
An eGFP-positive single DRG neuron was picked up with a glass capillary containing 18 μl lysis buffer [1× First strand buffer, 0.5 mg/ml bovine serum albumin, RNase inhibitor, 0.6% NP40, 0.6% Tween 20, anti-sense primer for CFTR, anti-sense primer for glyceraldehyde-3-phosphate dehydrogenase (GAPDH), dNTP and 1 mg/ml yeast tRNA], incubated at 70°C for 1 min to break the cell membrane. Cell lysate was incubated in the presence of DNase I at 37°C for 30 min to remove genomic DNAs and at 70°C for 10 min to inactivate DNase I. Then, the sample was incubated in a DTT solution containing SuperScript III reverse transcriptase (Invitrogen, Carlsbad, CA, USA) at 55°C for 40 min followed by at 70°C for 15 min. The gene expression was amplified by double PCRs. Initially, PCR was carried out in the reaction solution containing 10× PCR buffer, dNTPs, oligonucleotide, dimethyl sulfoxide [final concentration, 5% (v/v)], and 5 units of Taq polymerase (Fermentas, St. Leon-Roth, Germany)(final volume, 20 μl) with a PCR Thermal Cycler Dice (Takara, Otsu, Japan) programmed as follows: first step, 98°C for 4 min, the ensuring 40 cycles, 98°C for 1 s, 65°C for 15 s, and 72°C for 30 s. Subsequently, real-time PCR was performed using the first PCR products with a SYBR Green realtime PCR Master Mix (Toyobo, Osaka, Japan) and the Applied Biosystems 7900 real-time PCR detection system (ABI, Foster City, CA, USA) programmed as follows: first step, 98°C for 4 min; the ensuring 40 cycles, 98°C for 1 s, 65°C for 15 s, and 72°C for 30 s. The expression level of rat CFTR mRNA was normalized by that of GAPDH mRNA. Primers used for RT-PCR were as follows: 5'-AATATCCTTAGCCCCTCGGA-3' and 5'-TGGTGGAAACAATGGCACTA-3' for CFTR (accession number M89906) and 5'-CCTTCCGTGTTCCTACCCCCAAT-3' and 5'-CCTCTCTCTTGCTCTCAGTATCCTTGCT-3' for GAPDH (accession number BC059110).
Acutely dissociated DRG cells were fixed with 4% (w/v) paraformaldehyde, permeabilized with 0.3% (v/v) Triton X-100, and blocked with 10% (v/v) goat serum in phosphate buffered saline (PBS) at room temperature. Cells were reacted with a mouse monoclonal antibody for MAP2 (1:500), GFAP (1:400), S-100 (1:500) or fibronectin (1:500)(Chemicon, Temecula, CA, USA) and a rabbit polyclonal antibody for CFTR (1:200)(Chemicon) overnight at 4°C followed by a goat anti-rabbit IgG conjugated with Alexa 488 (Molecular Probes, Eugene, OR, USA) and a goat anti-mouse IgG conjugated with Alexa 633 (Molecular Probes) for 60 min at room temperature. Fluorescence-labeled cells were visualized with a confocal scanning laser microscope (Axiovert/LSM510; Carl Zeiss, Oberkochen, Germany).
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