A role of TRPA1 in mechanical hyperalgesia is revealed by pharmacological inhibition
© Petrus et al; licensee BioMed Central Ltd. 2007
Received: 29 October 2007
Accepted: 17 December 2007
Published: 17 December 2007
Mechanical hyperalgesia is a clinically-relevant form of pain sensitization that develops through largely unknown mechanisms. TRPA1, a Transient Receptor Potential ion channel, is a sensor of pungent chemicals that may play a role in acute noxious mechanosensation and cold thermosensation. We have developed a specific small molecule TRPA1 inhibitor (AP18) that can reduce cinnameldehyde-induced nociception in vivo. Interestingly, AP18 is capable of reversing CFA-induced mechanical hyperalgesia in mice. Although TRPA1-deficient mice develop normal CFA-induced hyperalgeisa, AP18 is ineffective in the knockout mice, consistent with an on-target mechanism. Therefore, TRPA1 plays a role in sensitization of nociception, and that compensation in TRPA1-deficient mice masks this requirement.
Sensory neurons of the dorsal root ganglia (DRGs) can detect environmental changes through projections in the skin. Among these primary afferent fibers, nociceptors play an important role in sensing noxious levels of mechanical and thermal stimuli . One of the basic properties of nociceptors is their sensitization in response to injury and inflammation. For example, an innocuous stimulus such as a warm shower can become painful after sunburn, and this is called allodynia. Indeed, cold or mechanical allodynia are serious medical problems for patients suffering from neuropathic pain [2, 3]. Hyperalgesia describes a similar condition, defined as an increased response to an already painful stimulus due to injury or inflammation.
TRPV1, a member of the Transient Receptor Potential family of cation channels, is activated by heat, low pH, and capsaicin . TRPV1 is expressed in nociceptors, and is sensitized in response to a variety of signal transduction pathways activated during inflammation [5, 6]. TRPV1-deficient mice display negligible inflammatory heat hyperalgesia [7, 8]. Much less is known about molecules involved in mechanical hyperalgesia.
Originally characterized as a noxious cold-activated ion channel, TRPA1 is expressed in the same sensory neurons as TRPV1, and is activated directly by diverse reactive chemicals via covalent modification, and indirectly through G-protein coupled receptors [9–12]. TRPA1 is required to sense these noxious reactive chemicals in vivo; however, whether TRPA1 is required to sense acute noxious cold and mechanical stimulus is not yet settled [13–17]. Here, we examine the consequences of acute block of peripheral TRPA1 for pain transduction, focusing on mechanical hyperalgesia.
The consequence of acute pharmacological block of protein activity in adult animals sometimes deviates from the effects of germline ablation of the encoding gene. For example, it was recently shown that pharmacological inhibition of TRPV1 induces hyperthermia in wildtype mice in a TRPV1-dependent manner, although mice born genetically-deficient in TRPV1 do not display a hyperthermic phenotype [23, 24]. Using a small molecule inhibitor of TRPA1 we have demonstrated a novel role of TRPA1 in mechanical hyperalgesia. AP18 is ineffective in reversing CFA-induced mechanical hyperalgesia in TRPA1-deficient mice, suggesting an on-target mechanism for AP18. What compensatory mechanisms may account for the normal mechanical hyperalgesia observed in TRPA1-deficient mice? Upregulation of another protein that plays a similar role as TRPA1 (molecular compensation) is one explanation. TRPA1 is the only member of the TRPA family in mammals, and TRPV1, another nocisensor expressed in pain-sensing neurons, does not appear to be upregulated in TRPA1-deficient mice, although the two channels functionally interact [16, 17, 25]. TRPV4, a related ion channel, has been implicated in some forms of mechanical hyperalgesia and can be an intriguing candidate . However, whether TRPV4 expression is required in skin cells or DRGs is unsettled . Cellular compensation (i.e. a change in the composition of neuronal subtypes or in neuronal connectivity) is another potential explanation for the normal hyperalgesia observed in TRPA1-deficient mice. Furthermore, it is possible that TRPA1 is involved in the maintenance of mechanical hyperalgesia rather than its induction. In this case, normal development of mechanical hyperalgesia is expected in TRPA1-deficient mice, but compensation could contribute to maintenance of the condition.
TRPA1 is specifically expressed in nociceptors, its transcripts are upregulated in response to CFA in rats, and inflammatory signals sensitize the channel, consistent with TRPA1 being involved in hyperalgesia [21, 28, 29]. The mechanism by which TRPA1 contributes specifically to mechanosensation is less clear . It is possible that mammalian TRPA1 is directly activated by mechanical forces, although no such data is currently present. Alternatively, mechanical damage could indirectly activate TRPA1 through release of reactive compounds or intracellular calcium (both of which can directly activate mammalian TRPA1) [11, 12, 31]. One other possibility is that TRPA1 is involved in modulation of mechanosensory signaling, and not directly involved with mechanotransduction. Interestingly, the ortholog of mammalian TRPA1 in C. elegans can be activated by pressure applied through a recording pipette when expressed in heterologous cells (although it is also not clear if cTRPA1 is directly or indirectly responding to this mechanical force) and plays an essential role in mechanosensory behaviour .
In conclusion, this study highlights a novel role of mouse TRPA1 in mechanical hyperalgesia, and underscores the importance of considering both acute pharmacological block and genetic ablations in assigning gene function.
FLIPR Screen and Calcium imaging
CHO cells expressing human TRPA1 were plated into 384-well plates at a concentration of ~8,000 cells/well. Cells were transferred to phosphate-buffered saline (PBS) and loaded with the calcium sensitive dye FLUO-4 using the FLIPR Calcium 3 Assay Kit (Molecular Devices, Sunnyvale, CA) one hour prior to the assay. Assays were run using the FLIPR2 (Molecular Devices, Sunnyvale, CA). All compounds were diluted into PBS from a high concentration DMSO-based stock solution and added during data collection with the FLIPR2 internal pipette head. Final DMSO concentrations never exceeded 0.5%. Ratiometric Ca2+ imaging on tet-inducible mouse TRPA1 in CHO cells was performed as described.
Xenopus oocyte excised patches
Human TRPA1 was cloned into the pOX expression vector and cRNA transcripts were produced using the T3 mMessage Machine kit (Ambion, TX). Mature defolliculated Xenopus oocytes were injected with 50 nL of human TRPA1 cRNA at ~1 μg/μL. Oocytes were incubated in ND96 (96 mM NaCl, 2 mM KCl, 1 mM MgCl2, 1.8 mM CaCl2, 5 mM HEPES, pH 7.4, supplemented with Na-pyruvate (2.5 mM), penicillin (100 u/mL) and streptomycin (100 μg/mL) for three to five days to ensure sufficient TRPA1 expression. Vitelline envelopes were mechanically removed prior to experiments. Recordings were made under voltage clamp from excised patches in the inside-out configuration at room temperature with 1–1.5 MΩ pipettes. The bath ground was isolated using an agar bridge. Capacitance and series resistance were compensated and solutions that eliminate native calcium-activated chloride currents were used (Patch electrode (in mM): 140 NaMES, 4 NaCl, 1 EGTA, 10 HEPES, pH 7.2; bath solution: 140 KMES, 4 KCl, 1 EGTA, 10 HEPES, pH 7.2). Compounds were added to the bath solution. Currents were recorded using a Multiclamp 700B amplifier and the pCLAMP acquisition suite.
Mice of 6–16 weeks in age and 150–250 g Sprague Dawley rats were used for all behavioural assays. C57BL6/J mice were used, except in Figure 3b: TRPA1-deficient mice and WT littermates were on mixed genetic background . Animals were acclimated for 20–60 min to their testing environment prior to all experiments. Student's t- test was used for all statistical calculations. All error bars represent standard error of the mean (SEM). Hargreaves apparatus (Plantar Analgesia meter) and Von Frey apparatus (Dynamic Plantar Aesthesiometer) are from UGO Basile. Mechanical or thermal hyperalgesia assays were performed as described [7, 34]. Briefly, mice were acclimated for 60 minutes to the testing environment prior to all experiments. Baseline responses were measured first and then 10 nM BK in 20 μl was injected to the skin of left hindpaws. Von Frey threshold or paw withdrawal latency was measured at 5, 15 and 30 minutes post injection. 1 mM of AP18 was coinjected to left hindpaws to test its analgesic effect. For CFA-induced hyperalgesia testing, 10 μg CFA in 10 μL was injected into mice and 50 μg in 100 μL (1:1 emulsion of mineral oil and saline) was injected into rats and 24-hour measurements were performed [7, 21, 35]. 1 mM of AP18 was injected in 10 μl. Control and AP18 solutions were in PBS, 0.5% Tween80 and 1% DMSO. Before the measurements, animals were re-acclimated to the environment for 20–60 minutes. Different time points were used for experiments with CFA-injected animals (30 min, 1, 1 1/2, 2 and 4 hr after AP18 injection).
All chemicals were purchased from Sigma-Aldrich unless otherwise described. Capsaicin was purchased from Fluka.
This research was supported by NIH grants DE016927, NS046303, and The Novartis Research Foundation. We thank D. Corey for TRPA1-deficient mice; Adrienne Dubin, Nancy Hong, Lindsey Macpherson, and Gina Story for their contributions.
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