- Open Access
Long-term potentiation at C-fibre synapses by low-level presynaptic activity in vivo
© Drdla and Sandkühler; licensee BioMed Central Ltd. 2008
Received: 02 April 2008
Accepted: 28 May 2008
Published: 28 May 2008
Inflammation, trauma or nerve injury trigger low-level activity in C-fibres and may cause long-lasting hyperalgesia. Long-term potentiation (LTP) at synapses of primary afferent C-fibres is considered to underlie some forms of hyperalgesia. In previous studies, high- but not low-frequency conditioning stimulation of C-fibres has, however, been used to induce LTP in pain pathways. Recently we could show that also conditioning low-frequency stimulation (LFS) at C-fibre intensity induces LTP in vitro as well as in the intact animal, i.e. with tonic descending inhibition fully active. In the slice preparation, this form of LTP requires a rise in postsynaptic Ca2+-concentration and activation of Ca2+-dependent signalling pathways. Here, we investigated the signalling mechanisms underlying this novel form of LTP in vivo. We found that the signal transduction pathways causing LFS-induced LTP in vivo include activation of neurokinin 1 and N-methyl-D-aspartate receptors, rise of [Ca2+]i from intracellular stores and via T-type voltage-dependent Ca2+ channels, activation of phospholipase C, protein kinase C and Ca2+-calmodulin dependent kinase II. These pathways match those leading to hyperalgesia in behaving animals and humans. We thus propose that LTP induced by low-level activity in C-fibres may underlie some forms of hyperalgesia.
LTP at the first synapse in pain pathways is considered to underlie some forms of pain amplification e.g. after trauma, inflammation or nerve injury . A strong rise in postsynaptic calcium ion concentration triggering Ca2+-dependent signal transduction pathways is required for LTP induction [2–4]. Consequently, high-frequency (~100 Hz), burst-like stimulation protocols were previously used to induce LTP at virtually all synapses studied so far.
Low-level activity between 1–10 imp·s-1 rather than high frequency bursts are, however, typical for C-fibre discharges during inflammation, trauma or wound healing. Presynaptic activity at these low frequencies is considered inadequate to cause a sufficiently strong rise in postsynaptic [Ca2+]i for potentiation of synaptic strength. In fact, low-level presynaptic activity was either ineffective or induced synaptic long-term depression (LTD) rather than LTP in previous studies.
We have recently discovered that in a spinal cord slice preparation with long dorsal roots intact LFS of dorsal roots at C-fibre intensity induces LTP which involves a rise in postsynaptic [Ca2+]i and Ca2+-dependent signal transduction pathways . In the intact animal spinal dorsal neurons are, however, under a powerful tonic inhibition arising from supraspinal, descending pathways [5, 6]. This inhibition is inevitably lost in the in vitro situation and could thereby facilitate LTP-induction. And indeed, removal of descending, putatively inhibitory pathways by spinalisation is required for the induction of LTP by either pinching or noxious heating of the skin . On the other hand, we have shown recently that in the intact animal, LTP can be induced by LFS as well as by subcutaneous capsaicin or formalin injections also . Here, we further characterised this novel LFS-induced LTP at C-fibre synapses in the intact animal.
Preparation of the animals
After obtaining approval from the Institutional Animal Care Comitee (Austrian Federal Ministry for Education, Science and Culture), experiments were performed on adult male Sprague Dawley rats (200 – 300 g) obtained from a local breeding facility.
Animals were intubated using a 16 G cannula and mechanically ventilated (Siemens Servoventilator 900C). Isoflurane in two thirds N2O and one third O2 was used to induce (4 vol% inspiratory) and maintain (1.5 vol% expiratory) anaesthesia. Surgical level of anaesthesia was verified by stable arterial blood pressure and the absence of paw withdrawal reflexes after pinching the paw. Surgical procedures have been described in more detail elsewhere . Briefly, the right femoral vein and artery were cannulated to allow i.v. infusions and to monitor arterial blood pressure. Muscle relaxation was achieved by 2 μg·kg-1·h-1 pancuronium bromide i.v The left sciatic nerve was dissected free for bipolar electrical stimulation using a silver hook electrode. Lumbar segments L4 and L5 were exposed by laminectomy. The dura mater was incised and reflected. Two metal clamps were used for fixation of the vertebral column in a stereotactic frame. An agarose pool was formed around the exposed spinal segments. The spinal segments were covered with artificial cerebrospinal fluid which was carefully removed before application of drugs (see below). The exposed sciatic nerve was covered with warm paraffin oil. At the end of the experiment animals were decapitated in deep anaesthesia and the spinal cord removed to locate the recording site (see next paragraph).
Electrophysiological recordings were performed as described elsewhere . Briefly, C-fibre-evoked field potentials were recorded with glass electrodes (2–3 MΩ) in laminae I and II of the spinal cord dorsal horn in response to stimulation of the ipsilateral sciatic nerve. Electrodes were driven by a microstepping motor (Narishige Scientific Instrument, Japan). Recordings were made with an ISO-DAM-amplifier (World Precision Instruments Inc., Sarasota, FL, USA) using a band width filter of 0.1 – 1000 Hz. Signals were monitored on a digital oscilloscope (Tektronix TDS 210) and digitized at a sampling rate of 5 kHz by an A/D converter (DigiData 1200 Series Interface). Field potentials may be evoked by A- or C-fibres. The different components can easily be distinguished by their thresholds and latencies. C-fibre evoked field potentials have high thresholds (> 5–6 V) and long latencies (90 – 150 ms, corresponding to the conduction velocity of < 1.2 m·s-1). The potentials were not abolished by spinalisation or muscle relaxation, strongly suggesting that these signals are evoked by activation of primary afferent C-fibres. This is in line with a report by Schouenborg , suggesting that field potentials are generated monosynaptically by synapses between primary afferent fibres and second order neurons. A polysynaptic contribution to the signals can, however, not be fully excluded. As test stimuli, single pulses (0.5 ms, 25 V, which is suprathreshold for the activation of C-fibres) were delivered to the sciatic nerve every 5 minutes using an electrical stimulator (Iso-Stim 01D, npi electronic, Germany). Stable responses for 1 hour served as control. LTP of C-fibre evoked field potentials was induced by low frequency stimulation (LFS, 2 Hz, 0.1 ms pulses, 60 V, 2 min). After conditioning stimulation, test pulses were applied again to the sciatic nerve at 5 min intervals for up to 7 hours.
Rhodamine B (0.2%, Sigma) was added to the pipette solution which consisted of (in mM) NaCl 135, KCl 5.4, CaCl2 1.8, Hepes 10 and MgCl2 1. For identification of the recording position, pressure was applied to the electrode (300 mbar, 1 min) at the end of each electrophysiological experiment. The spinal cord was removed, cryo-fixed and the rhodamine B spot was localised under a fluorescence microscope. Only those experiments where the tip of the recording electrode was located in lamina I or II were included into the study.
Pancuronium bromide (Pancuronium-ratiopharm®, Ratiopharm GmBH, Ulm, Germany) was given as an i.v. infusion (2 μg·kg-1·h-1). The competitive N-methyl-D-aspartate (NMDA) receptor antagonist D(-)-2-Amino-5-phosphonopentanoic acid (D-AP5, 100 μM, Sigma) and the T-type voltage-dependent Ca2+ channel (VDCC) blocker mibefradil dihydrochloride hydrate (5 mM, Sigma) were dissolved in 0.9% NaCl. The protein kinase C (PKC) blocker chelerythrine chloride (800 μM, Sigma) was dissolved in ddH2O. The Ca2+-calmodulin dependent kinase II (CaMKII) blocker 2- [N-(2-hydroxyethyl)]-N-(4methoxybenzenesulfonyl)]amino-N-(4-chlorocinnamyl)-N-methylbenzylamine (KN-93, 400 μM, Sigma), the phospholipase C (PLC) blocker 1- [6-[((17β)-3-Methoxyestra-1,3,5-trien-17-yl)amino]hexyl]-1H-pyrrole-2,5-dione (U-73122, 500 μM, Sigma), the neurokinin receptor 1 (NK1 receptor) blocker cis-2-(Diphenylmethyl)-N- [(2-iodophenyl)methyl]-1azabicyclo [2.2.2]octan-3-amine oxalate salt (L-703,606, 5 mM, Sigma) and the ryanodine receptor blocker dantrolene (500 μM, Tocris) were dissolved in DMSO. Dantrolene was further dissolved in standard solution titrated to a pH of 9.5. All other drugs were further dissolved in artificial cerebrospinal fluid to obtain desired concentrations as indicated and applied directly onto the spinal cord , starting 30 min prior LFS.
Data analysis and statistics
The area under the curve of C-fibre evoked field potentials was determined offline using the software Clampfit 9.0 (Molecular Devices Inc.). For the electrophysiological experiments, responses were normalised for each rat. A one way repeated measures analysis of variance (One way RM-ANOVA) was performed to compare the different experimental protocols and treatments. ANOVA was corrected by the Bonferroni adjustment. A p-value < 0.05 was considered statistically significant. Values are expressed as mean ± standard error of mean (SEM).
Results and discussion
Summary of results
C-fibre response (% control)
160% ± 13%*
150% ± 14%*
108% ± 14% n.s.
88% ± 15%*
103% ± 14% n.s.
107% ± 10% n.s.
103% ± 7% n.s.
107% ± 8% n.s
99% ± 5% n.s
Upon stimulation substance P is released from C-fibre afferents, leading to activation of neurokinin-1 (NK1) receptors, mainly in laminae I/II neurons of the spinal dorsal horn . Most NK1-expressing lamina I neurons have a supraspinal projection . Blockade of spinal NK1 receptors attenuates thermal and mechanical hyperalgesia [15–17]. In the present study, blocking spinal NK1 receptors with L-703,606 (5 mM) not only fully blocked LTP induction but rather led to LFS-induced LTD of C-fibre evoked field potentials (Fig 2B; Table 1).
The thresholds for the induction of LTP and LTD have been suggested to be tuned narrowly. Changes in the level of elevation of [Ca2+]i might shift the threshold from LTP towards LTD . The polarity of synaptic plasticity further depends upon the magnitude , the temporal pattern  and the mode of postsynaptic Ca2+ elevation . These parameters are all relevant for activation of distinct Ca2+-dependent signal transduction pathways involving protein phosphatases and kinases. Interestingly, blockade of NK1 receptors but not of VDCCs or NMDA receptors (see below) might critically change at least one of these characteristics of the rise in [Ca2+]i, shifting synaptic plasticity from LTP towards LTD. This illustrates that blocking different routes of [Ca2+]i rise may have differential effects on synaptic plasticity.
In superficial spinal dorsal horn neurons [Ca2+]i may be increased by the release of Ca2+ from intracellular stores  via activation of either ryanodine or inositol 1,4,5-trisphosphate (IP3) receptors. Ryanodine receptors may be activated by Ca2+ which may, for example, enter the cell through VDCCs, leading to Ca2+-induced Ca2+ release from ryanodine sensitive stores . In the present study, spinal ryanodine receptors were blocked by dantrolene (500 μM), a clinically used ryanodine receptor antagonist. This abolished LTP induction in all animals tested (Fig 2C; Table 1). Intrathecal injection of dantrolene has previously been shown to reduce nociceptive behaviour caused by spinal application of a NK1 receptor agonist .
Another trigger for Ca2+ release from intracellular stores is the activation of IP3 receptors. IP3 receptors are activated by IP3, a product formed by hydrolysis of phosphatidylinositol 4,5 bisphosphate upon activation of the phosphoinosite system. It starts with activation of phospholipase C (PLC) by the alpha subunit of a Gq-protein . Activation of PLC in the spinal cord is essential for hyperalgesia. For example, mechanical hyperalgesia induced by intraplantar injection of endothelin 1, a peptide produced by many different cell types, requires activation of spinal PLC . Furthermore, intrathecal pre-treatment with a PLC inhibitor reduced the second-phase in the formalin test . In the present study, inhibition of PLC in spinal cord by the selective blocker U-73122 (500 μM) completely prevented induction of LTP by LFS (Fig. 2D; Table 1).
PLC activation also leads to the production diacylglycerol (DAG) which in turn activates protein kinase C (PKC). At least 12 isoforms of this enzyme can be distinguished, many of those are present in superficial laminae of the spinal cord dorsal horn . PKC plays a crucial role in the development of hyperalgesia. Activation of spinal PKC by phorbol esters evokes thermal hyperalgesia and mechanical allodynia in awake rats . An involvement of PKC in inflammatory pain has also been shown. For example, PKC inhibitors attenuate hyperalgesia after intradermal injection of capsaicin or formalin [28, 29]. PKC may phosphorylate the NMDA receptor at distinct phosphorylation sites, thereby increasing channel activity [30, 31] or removing the Mg2+ block . Here, blockade of spinal PKC by chelerythrine (800 μM) abolished induction of LTP by LFS in all animals tested (Fig. 2E; Table 1).
Activation of Ca2+-permeable NMDA receptors in spinal dorsal horn is an essential step for the induction of several forms of hyperalgesia and allodynia [33–35]. However, NMDA receptors are normally blocked near the resting membrane potential by Mg2+. One might speculate that LFS is not sufficient to activate NMDA receptors, unless the Mg2+ block is removed e.g. by phosphorylation via PKC. And indeed, LFS-induced LTP required activation of NMDA receptors, as blockade of spinal NMDA receptors with the competitive NMDA receptor antagonist D-AP5 (100 μM) prevented LTP induction (Fig 2F; Table 1). This is similar to findings of previous studies showing that NMDA receptor activation is also required for high frequency stimulation (HFS)-induced LTP in the spinal cord dorsal horn [36–38, 3].
A rise in [Ca2+]i may lead to Ca2+ binding to calmodulin, which in turn activates Ca2+-calmodulin dependent kinase II (CaMKII). CaMKII undergoes a Ca2+-dependent autophosphorylation, resulting in Ca2+-independent activity . One of its main targets is the GluR1 subunit of the AMPA receptor, which gets phosphorylated at Ser831 . This leads to an increased channel conductance and thus synaptic strength . Activation of spinal CaMKII is required for some forms of hyperalgesia [42–44]. For example, intrathecal pre-treatment with a CaMKII inhibitor prevents thermal hyperalgesia and mechanical allodynia after injection of complete Freund's adjuvant into a hindpaw . Inhibition of CaMKII blocks induction of HFS-induced LTP in the spinal cord dorsal horn .
In the present study, we investigated signal transduction mechanisms underlying LFS-induced LTP in the spinal cord in vivo. We could show that pathways required for LTP induction match those involved in hyperalgesia. This suggests that LFS-induced LTP at spinal synapses of C-fibres may be a mechanism of pain amplification in behaving animals and perhaps human subjects .
Conditioning electrical stimulation of C-fibres at low frequencies induces LTP at the first nociceptive synapses in the spinal cord dorsal horn in slice preparations as well as in intact animals [, present study]. The signal transduction pathways underlying this novel form of LTP have been previously investigated in vitro only . Here we demonstrate that pathways underlying the induction of LFS-induced LTP in vivo fully match those in the in vitro slice preparation suggesting that the same cellular elements are involved despite the very different experimental conditions. The absence or presence of descending modulatory pathways or general anaesthesia does apparently not affect the principle mechanisms of LFS-induced synaptic plasticity in superficial spinal dorsal horn.
This work was supported by the FWF grant #18129 to JS.
- Sandkühler J: Understanding LTP in pain pathways. Mol Pain 2007, 3: 9–9. 10.1186/1744-8069-3-9PubMed CentralView ArticlePubMedGoogle Scholar
- Malenka RC, Kauer JA, Zucker RS, Nicoll RA: Postsynaptic calcium is sufficient for potentiation of hippocampal synaptic transmission. Science 1988, 242: 81–84. 10.1126/science.2845577View ArticlePubMedGoogle Scholar
- Ikeda H, Heinke B, Ruscheweyh R, Sandkühler J: Synaptic plasticity in spinal lamina I projection neurons that mediate hyperalgesia. Science 2003, 299: 1237–1240. 10.1126/science.1080659View ArticlePubMedGoogle Scholar
- Ikeda H, Stark J, Fischer H, Wagner M, Drdla R, Jäger T, Sandkühler J: Synaptic amplifier of inflammatory pain in the spinal dorsal horn. Science 2006, 312: 1659–1662. 10.1126/science.1127233View ArticlePubMedGoogle Scholar
- Basbaum AI, Fields HL: Endogenous pain control mechanisms: review and hypothesis. Ann Neurol 1978, 4: 451–462. 10.1002/ana.410040511View ArticlePubMedGoogle Scholar
- Sandkühler J: The organization and function of endogenous antinociceptive systems. Prog Neurobiol 1996, 50: 49–81.View ArticlePubMedGoogle Scholar
- Sandkühler J, Liu X: Induction of long-term potentiation at spinal synapses by noxious stimulation or nerve injury. Eur J Neurosci 1998, 10: 2476–2480. 10.1046/j.1460-9568.1998.00278.xView ArticlePubMedGoogle Scholar
- Schouenborg J: Functional and topographical properties of field potentials evoked in rat dorsal horn by cutaneous C-fibre stimulation. J Physiol 1984, 356: 169–192.PubMed CentralView ArticlePubMedGoogle Scholar
- Beck H, Schröck H, Sandkühler J: Controlled superfusion of the rat spinal cord for studying non-synaptic transmission: an autoradiographic analysis. J Neurosci Methods 1995, 58: 193–202. 10.1016/0165-0270(94)00176-HView ArticlePubMedGoogle Scholar
- Berridge MJ: Neuronal calcium signaling. Neuron 1998, 21: 13–26. 10.1016/S0896-6273(00)80510-3View ArticlePubMedGoogle Scholar
- Todorovic SM, Meyenburg A, Jevtovic-Todorovic V: Mechanical and thermal antinociception in rats following systemic administration of mibefradil, a T-type calcium channel blocker. Brain Res 2002, 951: 336–340. 10.1016/S0006-8993(02)03350-4View ArticlePubMedGoogle Scholar
- Dogrul A, Gardell LR, Ossipov MH, Tulunay FC, Lai J, Porreca F: Reversal of experimental neuropathic pain by T-type calcium channel blockers. Pain 2003, 105: 159–168. 10.1016/S0304-3959(03)00177-5View ArticlePubMedGoogle Scholar
- Go VL, Yaksh TL: Release of substance P from the cat spinal cord. J Physiol 1987, 391: 141–167.PubMed CentralView ArticlePubMedGoogle Scholar
- Spike RC, Puskár Z, Andrew D, Todd AJ: A quantitative and morphological study of projection neurons in lamina I of the rat lumbar spinal cord. Eur J Neurosci 2003, 18: 2433–2448. 10.1046/j.1460-9568.2003.02981.xView ArticlePubMedGoogle Scholar
- Traub RJ: The spinal contribution of substance P to the generation and maintenance of inflammatory hyperalgesia in the rat. Pain 1996, 67: 151–161. 10.1016/0304-3959(96)03076-XView ArticlePubMedGoogle Scholar
- Sakurada C, Sakurada S, Katsuyama S, Sasaki J, Tan-No K, Sakurada T: Involvement of tachykinin NK1 receptors in nociceptin-induced hyperalgesia in mice. Brain Res 1999, 841: 85–92. 10.1016/S0006-8993(99)01800-4View ArticlePubMedGoogle Scholar
- King T, Gardell LR, Wang R, Vardanyan A, Ossipov MH, Philip Malan T Jr., Vanderah TW, Hunt SP, Hruby VJ, Lai J, Porreca F: Role of NK-1 neurotransmission in opioid-induced hyperalgesia. Pain 2005, 116: 276–288. 10.1016/j.pain.2005.04.014PubMed CentralView ArticlePubMedGoogle Scholar
- Lisman JE: Three Ca2+ levels affect plasticity differently: the LTP zone, the LTD zone and no man's land. J Physiol 2001, 532: 285–285. 10.1111/j.1469-7793.2001.0285f.xPubMed CentralView ArticlePubMedGoogle Scholar
- Lisman J: A mechanism for the Hebb and the anti-Hebb processes underlying learning and memory. Proc Natl Acad Sci USA 1989, 86: 9574–9578. 10.1073/pnas.86.23.9574PubMed CentralView ArticlePubMedGoogle Scholar
- Bi GQ, Poo MM: Synaptic modifications in cultured hippocampal neurons: dependence on spike timing, synaptic strength, and postsynaptic cell type. J Neurosci 1998, 18: 10464–10472.PubMedGoogle Scholar
- Nishiyama M, Hong K, Mikoshiba K, Poo MM, Kato K: Calcium stores regulate the polarity and input specificity of synaptic modification. Nature 2000, 408: 584–588. 10.1038/35046067View ArticlePubMedGoogle Scholar
- Álvarez-Vega M, Baamonde A, Hidalgo A, Menéndez L: Effects of the calcium release inhibitor dantrolene and the Ca2+-ATPase inhibitor thapsigargin on spinal nociception in rats. Pharmacology 2001, 62: 145–150. 10.1159/000056087View ArticlePubMedGoogle Scholar
- Delmas P, Crest M, Brown DA: Functional organization of PLC signaling microdomains in neurons. Trends Neurosci 2004, 27: 41–47. 10.1016/j.tins.2003.10.013View ArticlePubMedGoogle Scholar
- Motta EM, Calixto JB, Rae GA: Mechanical hyperalgesia induced by endothelin-1 in rats is mediated via phospholipase C, protein kinase C, and MAP kinases. Exp Biol Med (Maywood ) 2006, 231: 1141–1145.Google Scholar
- Coderre TJ: Contribution of protein kinase C to central sensitization and persistent pain following tissue injury. Neurosci Lett 1992, 140: 181–184. 10.1016/0304-3940(92)90097-QView ArticlePubMedGoogle Scholar
- Polgár E, Fowler JH, McGill MM, Todd AJ: The types of neuron which contain protein kinase C gamma in rat spinal cord. Brain Res 1999, 833: 71–80. 10.1016/S0006-8993(99)01500-0View ArticlePubMedGoogle Scholar
- Palecek J, Paleckova V, Willis WD: The effect of phorbol esters on spinal cord amino acid concentrations and responsiveness of rats to mechanical and thermal stimuli. Pain 1999, 80: 597–605. 10.1016/S0304-3959(98)00250-4View ArticlePubMedGoogle Scholar
- Sluka KA, Willis WD: The effects of G-protein and protein kinase inhibitors on the behavioral responses of rats to intradermal injection of capsaicin. Pain 1997, 71: 165–178. 10.1016/S0304-3959(97)03371-XView ArticlePubMedGoogle Scholar
- Wajima Z, Hua XY, Yaksh TL: Inhibition of spinal protein kinase C blocks substance P-mediated hyperalgesia. Brain Res 2000, 877: 314–321. 10.1016/S0006-8993(00)02714-1View ArticlePubMedGoogle Scholar
- Lan JY, Skeberdis VA, Jover T, Grooms SY, Lin Y, Araneda RC, Zheng X, Bennett MV, Zukin RS: Protein kinase C modulates NMDA receptor trafficking and gating. Nat Neurosci 2001, 4: 382–390. 10.1038/86028View ArticlePubMedGoogle Scholar
- Liao GY, Wagner DA, Hsu MH, Leonard JP: Evidence for direct protein kinase-C mediated modulation of N-methyl-D-aspartate receptor current. Mol Pharmacol 2001, 59: 960–964.PubMedGoogle Scholar
- Chen L, Huang LY: Protein kinase C reduces Mg2+ block of NMDA-receptor channels as a mechanism of modulation. Nature 1992, 356: 521–523. 10.1038/356521a0View ArticlePubMedGoogle Scholar
- Woolf CJ, Thompson SWN: The induction and maintenance of central sensitization is dependent on N-methyl-D-aspartic acid receptor activation; implications for the treatment of post-injury pain hypersensitivity states. Pain 1991, 44: 293–299. 10.1016/0304-3959(91)90100-CView ArticlePubMedGoogle Scholar
- Ren K, Hylden JLK, Williams GM, Ruda MA, Dubner R: The effects of a non-competitive NMDA receptor antagonist, MK-801, on behavioral hyperalgesia and dorsal horn neuronal activity in rats with unilateral inflammation. Pain 1992, 50: 331–344. 10.1016/0304-3959(92)90039-EView ArticlePubMedGoogle Scholar
- Mao J, Price DD, Hayes RL, Lu J, Mayer DJ: Differential roles of NMDA and non-NMDA receptor activation in induction and maintenance of thermal hyperalgesia in rats with painful peripheral mononeuropathy. Brain Res 1992, 598: 271–278. 10.1016/0006-8993(92)90193-DView ArticlePubMedGoogle Scholar
- Liu XG, Sandkühler J: Long-term potentiation of C-fiber-evoked potentials in the rat spinal dorsal horn is prevented by spinal N-methyl-D-aspartic acid receptor blockage. Neurosci Lett 1995, 191: 43–46. 10.1016/0304-3940(95)11553-0View ArticlePubMedGoogle Scholar
- Svendsen F, Tjølsen A, Hole K: AMPA and NMDA receptor-dependent spinal LTP after nociceptive tetanic stimulation. Neuroreport 1998, 9: 1185–1190. 10.1097/00001756-199804200-00041View ArticlePubMedGoogle Scholar
- Liu XG, Sandkühler J: Activation of spinal N-methyl-D-aspartate or neurokinin receptors induces long-term potentiation of spinal C-fibre-evoked potentials. Neuroscience 1998, 86: 1209–1216. 10.1016/S0306-4522(98)00107-9View ArticlePubMedGoogle Scholar
- Giese KP, Fedorov NB, Filipkowski RK, Silva AJ: Autophosphorylation at Thr286 of the a calcium-calmodulin kinase II in LTP and learning. Science 1998, 279: 870–873. 10.1126/science.279.5352.870View ArticlePubMedGoogle Scholar
- Barria A, Muller D, Derkach V, Griffith LC, Soderling TR: Regulatory phosphorylation of AMPA-type glutamate receptors by CaM-KII during long-term potentiation. Science 1997, 276: 2042–2045. 10.1126/science.276.5321.2042View ArticlePubMedGoogle Scholar
- Derkach V, Barria A, Soderling TR: Ca2+/calmodulin-kinase II enhances channel conductance of a-amino-3-hydroxy-5-methyl-4-isoxazolepropionate type glutamate receptors. Proc Natl Acad Sci USA 1999, 96: 3269–3274. 10.1073/pnas.96.6.3269PubMed CentralView ArticlePubMedGoogle Scholar
- Fang L, Wu J, Lin Q, Willis WD: Calcium-calmodulin-dependent protein kinase II contributes to spinal cord central sensitization. J Neurosci 2002, 22: 4196–4204.PubMedGoogle Scholar
- Fang L, Wu J, Zhang X, Lin Q, Willis WD: Calcium/calmodulin dependent protein kinase II regulates the phosphorylation of cyclic AMP-responsive element-binding protein of spinal cord in rats following noxious stimulation. Neurosci Lett 2005, 374: 1–4. 10.1016/j.neulet.2004.10.014View ArticlePubMedGoogle Scholar
- Larsson M, Broman J: Pathway-specific bidirectional regulation of Ca2+/calmodulin-dependent protein kinase II at spinal nociceptive synapses after acute noxious stimulation. J Neurosci 2006, 26: 4198–4205. 10.1523/JNEUROSCI.0352-06.2006View ArticlePubMedGoogle Scholar
- Luo F, Yang C, Chen Y, Shukla P, Tang L, Wang LX, Wang ZJ: Reversal of chronic inflammatory pain by acute inhibition of Ca2+/calmodulin-dependent protein kinase II. J Pharmacol Exp Ther 2008, 325: 267–275. 10.1124/jpet.107.132167View ArticlePubMedGoogle Scholar
- Yang HW, Hu XD, Zhang HM, Xin WJ, Li MT, Zhang T, Zhou LJ, Liu XG: Roles of CaMKII, PKA and PKC in the induction and maintenance of LTP of C-fiber evoked field potentials in rat spinal dorsal horn. J Neurophysiol 2004, 91: 1122–1133. 10.1152/jn.00735.2003View ArticlePubMedGoogle Scholar
- Klein T, Magerl W, Hopf HC, Sandkühler J, Treede RD: Perceptual correlates of nociceptive long-term potentiation and long-term depression in humans. J Neurosci 2004, 24: 964–971. 10.1523/JNEUROSCI.1222-03.2004View ArticlePubMedGoogle Scholar
- Boehm J, Malinow R: AMPA receptor phosphorylation during synaptic plasticity. Biochem Soc Trans 2005, 33: 1354–1356. 10.1042/BST20051354View ArticlePubMedGoogle Scholar
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