Characterization of two Runx1-dependent nociceptor differentiation programs necessary for inflammatory versus neuropathic pain
- Omar Abdel Samad†1,
- Yang Liu†1,
- Fu-Chia Yang†1,
- Ina Kramer2, 3,
- Silvia Arber2, 3 and
- Qiufu Ma1Email author
© Samad et al; licensee BioMed Central Ltd. 2010
Received: 23 June 2010
Accepted: 30 July 2010
Published: 30 July 2010
The cellular and molecular programs that control specific types of pain are poorly understood. We reported previously that the runt domain transcription factor Runx1 is initially expressed in most nociceptors and controls sensory neuron phenotypes necessary for inflammatory and neuropathic pain.
Here we show that expression of Runx1-dependent ion channels and receptors is distributed into two nociceptor populations that are distinguished by persistent or transient Runx1 expression. Conditional mutation of Runx1 at perinatal stages leads to preferential impairment of Runx1-persistent nociceptors and a selective defect in inflammatory pain. Conversely, constitutive Runx1 expression in Runx1-transient nociceptors leads to an impairment of Runx1-transient nociceptors and a selective deficit in neuropathic pain. Notably, the subdivision of Runx1-persistent and Runx1-transient nociceptors does not follow the classical nociceptor subdivision into IB4+ nonpeptidergic and IB4- peptidergic populations.
Altogether, we have uncovered two distinct Runx1-dependent nociceptor differentiation programs that are permissive for inflammatory versus neuropathic pain. These studies lend support to a transcription factor-based distinction of neuronal classes necessary for inflammatory versus neuropathic pain.
The sense of pain is initiated through the detection and transduction of noxious stimuli by specialized sensory neurons (called nociceptors), which are located in dorsal root ganglia (DRG) and trigeminal ganglia [1–3]. Under pathological conditions, such as tissue inflammation and nerve injury, nociceptors and central relay neurons can be sensitized by multiple pathways, leading to long-lasting chronic pain states such as inflammatory pain and neuropathic pain [2, 4–8]. In the past decades, significant progress has been made in understanding the molecular and cellular bases of acute and chronic pain [9–13]. However, much less is known about how nociceptor features associated with different pain behaviors are established during development.
We and others have previously shown that Runx1, a Runt-domain transcription factor, plays a pivotal role in controlling nociceptor phenotypes and pain behaviors [14–17]. Runx1 is initially expressed in most, if not all, embryonic nociceptors marked by the expression of the neurotrophin receptor TrkA. During perinatal and postnatal development, Runx1 expression is retained in only a subset of nociceptors that switch off TrkA and turn on Ret, the receptor for the glial-derived growth factor family of neurotrophins. The remaining nociceptors switch off Runx1, most of which retain TrkA, although a subset of them expresses Ret . Analyses of conditional knockout mice in which Runx1 was removed in sensory precursors show that Runx1 is required for proper development of Ret-expressing nociceptors, including the expression of dozens of sensory channels and receptors that are essential for thermal pain, inflammatory pain, and neuropathic pain .
A key unsolved question is which Runx1-dependent differentiation programs control which individual pain behaviors. In this study, we first made a conditional Runx1 knockout at perinatal stages (around embryonic day 17 or E17). In these late knockout mice, the expression of a subset of Runx1-dependent genes was affected, namely those in Runx1-persistent nociceptors, whereas expression of a separate set of Runx1-dependent genes that are normally expressed in Runx1-transient nociceptors was largely unaffected. Interestingly, behavioral analyses showed that preferential defect in Runx1-persistent nociceptors in these late knockouts led to a selective impairment of inflammatory pain without affecting neuropathic pain. Second, we made conditional knock-in mice that drive constitutive Runx1 expression in most of the nociceptors. This manipulation led to the impairment of Runx1-transient nociceptors and a selective deficit in neuropathic pain. We thus uncover two distinct Runx1-dependent differentiation programs that contribute to inflammatory versus neuropathic pain. Together, our study provides new insight into the molecular and cellular bases of chronic pain.
Two Runx1-dependent differentiation programs revealed by analyzing late Runx1 conditional knockout mice: Runx1 F/F ;Nav1.8 Cre
To determine which Runx1-dependent differentiation programs control specific types of pain, we wanted to generate mice that contain a partial impairment of Runx1-dependent nociceptor phenotypes. We made an assumption that a conditional knockout of Runx1 at a late developmental stage may preferentially eliminate the expression of those genes that are expressed in Runx1-persistent nociceptors. To do this, we crossed mice carrying a conditional Runx1 allele  (referred to as Runx1 F ) with a Nav1.8 Cre mouse line that drove Cre expression from the Nav1.8 sodium channel promoter in most nociceptors at perinatal stages . We referred to these late Runx1 conditional knockout mice (Runx1 F/F ;Nav1.8 Cre ) as L-CKO mice. Runx1 F/F littermates were referred to as control. In lumbar DRG of L-CKO mice, Runx1 expression was not affected at E14.5, detected robustly at E16, but virtually absent at E17 (See Additional file 1). In wild-type lumbar DRG, the onset of Runx1 expression occurs at E12.5 . Thus, in L-CKO mice, Runx1 operates during a developmental window from E12.5 to E17. We previously used Wnt1 Cre mice  to make a Runx1 early conditional knockout (Runx1 F/F ;Wnt1 Cre ), which was referred here to as E-CKO mice, in which Runx1 was removed in sensory precursors before the onset of Runx1 expression  (Summarized in Fig. 1A). Since pain behavior was measured from the hindpaws, all nociceptor phenotype analyses were carried out in L4-L5 lumbar DRG. Total neuron number in lumbar DRG, as measured by the expression of the panneural gene SCG10 , was not changed in L-CKO mice in comparison with that in control mice (See Additional file 1), suggesting that neuronal survival is unaffected in this knockout.
Additional molecular and anatomical changes occurred in IB4+ nociceptors. First, Runx1 is required to suppress the expression of a set of peptidergic nociceptor markers, such as TrkA, the neuropeptide CGRP , and the acid-sensing channel DRASIC [14, 15, 17]. Expression of these markers was expanded into IB4+ neurons in E-CKO mice , and this expansion also occured in L-CKO mice (See Additional file 3). Second, IB4+ fibers innervated the superficial lamina of the dorsal spinal cord in both E-CKO and L-CKO mice, rather than the inner lamina in control mice  (See Additional file 4).
In contrast, development of a separate group of Runx1-dependent sensory neuron phenotypes was no longer affected in L-CKO mice. For example, expression of several Mrgpr class GPCRs (Mrgpra3, Mrgprb4, and Mrgprc11), which was eliminated in E-CKO mice [14, 24], was fully "restored" in L-CKO mice (Fig. 1C). Counting of Mrgprb4+ neurons in lumbar DRG showed no differences in control versus L-CKO mice (Fig. 1E). Expression of the capsaicin receptor TRPV1 is also partially "restored" in L-CKO mice. TRPV1 expression is allocated into two separate populations of DRG neurons . A small subset of DRG neurons express very high levels of TRPV1 expression (TRPV1high), whereas a larger subset of DRG neurons express TRPV1 at low levels . Analysis of E-CKO mice showed that Runx1 is required for TRPV1high expression, but dispensable for TRPV1low expression . In L-CKO mice, we found that TRPV1high expression was only reduced by 75%, meaning that there was a 25% of "restoration" in L-CKO mice (Fig. 1D-E). Thus, by comparing mutant phenotypes in E-CKO and L-CKO mice, we have uncovered two distinct Runx1-dependent nociceptor differentiation programs, A and B. Program A controls a set of nociceptor phenotypes that are impaired in both E-CKO and L-CKO mice. Program B controls a separate set of nociceptor phenotypes that are impaired in E-CKO mice, but unchanged in L-CKO mice. Some Runx1-dependent genes are controlled by both programs, as indicated by partial "restoration" of their expression in L-CKO mice, such as TRPV1high.
Program A and B Runx1 activities operate preferentially in Runx1-persistent and Runx-1 transient neurons, respectively
The second group of program A-dependent DRG neurons is marked by the expression of TRPM8, which is involved in cold sensation . Again, a majority of TRPM8+ neurons (nearly 80%) showed persistent Runx1 expression, with 9% of Runx1+ neurons coexpressing TRPM8 (Fig. 2A). TRPM8+ neurons do not belong to the classic set of nociceptors, shown both by the little or no coexpression with CGRP and by the lack of IB4 labeling [28, 29]. Thus, Runx1-persistent Mrgprd+ (IB4+) and TRPM8+ (IB4-negative) neurons represent two distinct populations of sensory neurons.
As mentioned above, TRPV1high expression is controlled by both programs. We found that 77% and 23% of TRPV1high neurons showed persistent and transient Runx1 expression, respectively (Fig. 2A), which matched well with 75% and 25% of TRPV1high expression controlled by programs A and B, respectively. We also found that Runx1 expression was detected in 48% of TRPV1low neurons (Fig. 2A), even though TRPV1low expression is independent of Runx1.
Conversely, based on our previous co-localization studies , program B-dependent genes were expressed mainly in Runx1-transient DRG neurons. Mrgpra3+ neurons have been implicated in transmitting itch evoked by chloroquine , and these neurons do not show persistent Runx1 expression . Mrgprb4+ neurons innervate exclusively the hairy skin , and again, these neurons do not show persistent Runx1 expression . Another program B gene Mrgprc11 is expressed in both Mrgpra3+ and Mrgprb4+ neurons . It should be noted that program B-dependent neurons can be either nonpeptidergic, such as Mrgprb4+ neurons , or peptidergic, such as 71% of Mrgprc11+ neurons (See Additional file 6). Thus in lumbar DRG, program A and program B Runx1 activities operate preferentially in Runx1-persistent and Runx1-transient DRG neurons, respectively, each of which represents a heterogeneous population of sensory neurons (Fig. 2B).
A selective deficit of inflammatory pain in L-CKO mice
We next assayed neuropathic pain induction in L-CKO mice. To that end, we used the spared nerve injury model (SNI)  and measured the heightened pain sensitivity in which normally innocuous tactile stimuli illicit a pain withdrawal response, a phenomenon termed mechanical allodynia that is a hallmark of neuropathic pain . We found that the development of mechanical allodynia, indicated by the substantial lowering of the paw withdrawal threshold, was not significantly different between control and L-CKO mice (Fig. 3C, ANOVA, p = 0.72), indicating a nearly complete retention of this type of neuropathic pain in L-CKO mice. This result was remarkable considering a virtual abolishment of SNI-induced neuropathic pain in E-CKO mice .
Inflammatory pain responses were assayed by measuring the development of mechanical allodynia and heat hyperalgesia after intraplantar injection of complete Freund's adjuvant (CFA). CFA-induced edema occurred normally in both control and L-CKO mice, as shown by similar degrees of swelling in the hindpaws (see Methods). Interestingly, the development of mechanical allodynia after CFA injection was markedly impaired in L-CKO mice, shown by a substantial elevation of the paw withdrawal threshold compared with that in control littermates (Fig. 3D). We next examined how CFA-induced heat hyperalgesia was affected, using the Hargreaves assay . Before CFA injection, the latency in response to radiant heat was comparable between control and L-CKO mice (Fig. 3E), consistent with the hot plate assay showing no acute heat pain deficit. However, CFA-induced heat hyperalgesia was significantly impaired in L-CKO mice, shown by a substantial increase in the latency in response to radiant heat (Fig. 3E). These results suggest that inflammatory pain is impaired in both E-CKO and L-CKO mice . Thus, the selective loss of program A activity in L-CKO mice leads to a selective defect in inflammatory pain, and the retention of program B activity is sufficient to establish heat pain and neuropathic pain.
Constitutive Runx1 expression in most nociceptors caused a selective impairment of Runx1-transient nociceptors
We next wanted to generate mice in which development of Runx1-transient nociceptors was impaired. To do this, we employed a genetic strategy that prevented Runx1 downregulation in most nociceptors. We used the conditional knock-in mice Tau-lox-STOP-lox-Runx1-IRES-nlsLacZ-neo , referred to as Tau-Runx1 F . In this mouse line, Runx1 expression is under the control of the panneuronal Tau promoter but is not activated until the 'STOP' cassette is removed through Cre-mediated recombination. We crossed this line with the Nav1.8 Cre transgenic mouse line , with resulting double heterozygous mice referred to as Tau-Runx1 F ;Nav1.8 Cre mice (See Additional file 7). In these mice, Nav1.8 Cre drove constitutive Runx1 expression in most peptidergic and nonpeptidergic nociceptors . Consistently, the number of Runx1+ neurons increased by 88% (See Additional file 7), implying that following constitutive Runx1 expression, a significant portion of presumably Runx1-transient sensory neurons must have survived. However, the total neuron number, marked by the expression of SCG10, was reduced by 25% (See Additional file 7 ), suggesting some degree of neuronal cell loss. Development of proprioceptors, marked by the expression of Parvalbumin (PV), and low-threshold mechanoceptors, marked by the expression of TrkB (the receptor for the brain-derived neurotrophic factor or BDNF), was unaffected (See Additional file 8).
Development of Runx1-transient neurons, however, was impaired in Tau-Runx1 F ;Nav1.8 Cre mice. For example, expression of a set of Runx1-dependent genes that are normally expressed in Runx1-transient neurons was eliminated, including Mrgpra3, Mrgprb4, and Mrgprc11 (Fig. 4B). The number of TRPV1low neurons was reduced by 65% in Tau-Runx1 F ;Nav1.8 Cre mice (Fig. 4A and 4E), consistent with our finding that 52% of TRPV1low expression is distributed in Runx1-transient neurons (Fig. 2).
TrkA+;CGRP+ peptidergic nociceptors represent a separate group of Runx1-transient nociceptors. We had previously used Islet1 Cre to drive constitutive Runx1 expression in all DRG neurons, which resulted in a suppression of CGRP at embryonic stages without affecting embryonic TrkA expression. Those mice died at birth . Tau-Runx1 F ;Nav1.8 Cre mice, on the other hand, survived into adulthood, and thus allowed us to examine postnatal development of TrkA+;CGRP+ neurons. We found that expression of TrkA was not detected in P30 lumbar DRG of Tau-Runx1 F ;Nav1.8 Cre mice, either by in situ hybridization or by immunostaining (Fig. 4C). Expression of CGRP was also greatly reduced (by 78%) (Fig. 4C and 4E). Therefore, development of a majority of peptidergic nociceptors is impaired in Tau-Runx1 F ;Nav1.8 Cre mice. The incomplete suppression of CGRP is consistent with the observation that in wild-type DRG, about 15% of DRG neurons showed detectable Runx1 expression (See Additional file 6), suggesting that Runx1-mediated CGRP suppression operates in a cell context-dependent manner. Altogether, constitutive expression of Runx1 led to a preferential impairment of Runx1-transient nociceptors.
A selective deficit in neuropathic pain in Tau-Runx1 F ;Nav1.8 Cre mice
Distinct modes of Runx1 activities in controlling sensory neuron development
We provide a new way to subdivide nociceptors based on persistent or transient Runx1 expression. Notably, dividing nociceptors based on Runx1 expression does not follow the classic subdivision of nociceptors, namely CGRP+ peptidergic nociceptors versus IB4+ non-peptidergic nociceptors [1, 23]. For example, while most Runx1-persistent nociceptors, including TRPM8+ and Mrgprd+ neurons, are nonpeptidergic [26, 28, 29], about 15% of adult CGRP+ neurons also show detectable Runx1 expression (See Additional file 6). Similarly, among Runx1-transient nociceptors, TrkA+ and 71% of Mrgprc11+ neurons are peptidergic (See Additional file 6) , whereas Mrgprb4+ neurons are nonpeptdergic . IB4 is also distributed in both Runx1-persistent and Runx1-transient nociceptors. For example, Runx1-persistent nociceptors consist of IB4-;TRPM8+ cold receptors and IB4+;Mrgprd+ polymodal neurons [26, 28, 29, 34]. Similarly, Runx1-transient neurons consist of IB4-;TrkA+ and IB4+; Mrgpra3/b4/c11+ neurons [23, 24, 31].
By analyzing how Runx1-persistent and Runx1-transient nociceptors were affected in various Runx1 mutant mice, we recognize several distinct modes of Runx1 activity in controlling nociceptor development. The first one is observed in Runx1-persistent Mrgprd+ neurons. Mrgprd expression was detected at E16 in L-CKO mice (See Additional file 2), but eliminated in adult L-CKO mice, implying that Runx1 is required to both establish and maintain these neuron identities (Fig. 1). The second one is observed in neurons expressing Mrgpra3, b4, and c11. Early Runx1 activity is necessary to establish the expression of these genes [14, 24], but subsequent Runx1 extinguishment is required to maintain these neurons (Fig. 4). Mechanistically, we reported previously that Runx1 switches from an activator at early stages to a repressor at postnatal stages in regulating Mrgpra3, b4, and c11 , thereby explaining why their expression can only be sustained in Runx1-transient nociceptors. The third mode of action is observed in TrkA+;CGRP+ peptidergic nociceptors. So far, it is unclear if Runx1 itself plays an essential role in these neurons. What is clear, however, is that elimination of Runx1 expression is absolutely essential for the development of TrkA+;CGRP+ nociceptors (Fig. 4) . In summary, dynamic Runx1 expression and activity play a crucial role in generating nociceptor diversity.
Uncovering two Runx1-dependent differentiation programs necessary for inflammatory and neuropathic pain
Our studies show that Runx1-dependent nociceptor differentiation can be divided into two programs. Program A operates preferentially in Runx1-persistent nociceptors, and controls nociceptor phenotypes that are impaired in both E-CKO and L-CKO mice. Program B operates mainly in Runx1-transient nociceptors and controls a separate set of nociceptor phenotypes that are impaired in E-CKO mice but unaffected in L-CKO mice. Behavior analyses show that programs A and B are required for inflammatory pain and neuropathic pain, respectively. First, impairment of both programs in E-CKO mice led to deficits in both inflammatory and neuropathic pain . Second, a selective defect in program A in L-CKO mice led to impaired inflammatory pain (CFA-induced mechanical allodynia and heat hyperalgesia), whereas the retention of program B activity in these mice is sufficient to establish neuropathic pain (SNI-induced mechanical allodynia). Third, constitutive Runx1 expression in Tau-Runx1 F ;Nav1.8 Cre mice led to impairment of Runx1-transient neurons, including program B-dependent nociceptors; these mice show a selective deficit in neuropathic pain.
Program A-dependent Runx1-persistent DRG neurons include Mrgprd+ polymodal nociceptors, TRPM8+ cold receptors, 75% of TRPV1high neurons, and others. Mrgprd+ neurons are partially required to establish mechanical hypersensitivity induced by inflammation . TRPV1 is essential for inflammatory heat hyperalgesia [36, 37]. Thus, the impairment of Mrgprd+ neurons and the loss of 75% of TRPV1high expression in L-CKO mice might contribute to the defect in CFA-induced inflammatory pain in these mice.
One surprising finding is that inflammatory pain can be established in the absence of TrkA signaling in nociceptors, despite numerous reports suggesting that TrkA signaling plays a crucial role in inflammatory pain control . We found that in Tau-Runx1 F ;Nav1.8 Cre mice, despite the fact that TrkA expression was absent and NGF-induced inflammatory pain was impaired, no marked changes in CFA-induced mechanical allodynia and heat hyperalgesia were observed. Two possibilities (not necessarily mutually exclusive) are worth considering. First, TrkA signaling could be one of redundant sensitization pathways activated by CFA injection. Alternatively, TrkA-signaling may operate in immune cells to control inflammatory pain, and those cells were untouched by Nav1.8 Cre .
The neuropathic pain impairement observed in Runx1 gain-of-function mice (Tau-Runx1 F ;Nav1.8 Cre mice) could be due to the loss of Program B-dependent genes or be additionally caused by the impairment of other Runx1-transient neurons, such as TrkA+/CGRP+ neurons (Fig. 4C). This complexity, however, does not affect one key conclusion of this study: Runx1-transient DRG neurons are critical for neuropathic pain, and Runx1-persistent neurons, which remain intact in Runx1 gain-of-function mice, are insufficient to allow neuropathic pain.
The neuropathic pain defect in our Runx1 gain-of-function mice seems to conflict with recent results from Abrahamsen et al., regarding the cellular basis for neuropathic pain. When using Nav1.8 Cre mice (made in the Wood lab) to ablate 85% of nociceptors, Abrahamsen et al. found that these mice showed no defect in neuropathic pain, indicating that Nav1.8-expressing neurons are dispensable for neuropathic pain . However, when we used Nav1.8 Cre mice (made in the Kuner lab) to drive Runx1 expression, neuropathic pain was impaired. This discrepancy could be due to the difference between cell ablation and gene knock-in approach. Alternatively, it could be due to a specificity difference between these two Cre lines. Wood's Nav1.8 Cre mice were made by the knock-in strategy , whereas Kuner's Nav1.8 Cre mice were made through a transgenic approach . This specificity difference is reflected by the fact that TRPM8+ neurons are unaffected in Wood's ablation experiment , whereas TRPM8 expression was absent in Runx1 L-CKO mice using Kuner's Nav1.8 Cre line (Fig. 1B). Future comprehensive comparisons of these two Cre lines will be important for the pain field.
Altogether, we have uncovered two distinct Runx1-dependent nociceptor differentiation programs. Program A operates preferentially in Runx1-persistent nociceptors and controls a set of nociceptor phenotypes necessary for inflammatory pain. Program B operates mainly in Runx1-transient nociceptors and controls a set of nociceptor phenotypes permissive for neuropathic pain. Importantly, the subdivision of Runx1-persistent versus Runx1-transient nociceptors does not follow classical subdivisions of nociceptors. Rather, our studies lend support to a transcription factor-based distinction of neuronal classes mediating inflammatory versus neuropathic pain.
The morning that vaginal plugs were observed was considered E0.5. For histochemical studies, adult mice at P60 (and P30 where specified) were used. For behavioral analyses, 2-3 month-old mutant and control mice were used. PCR-based genotyping was performed. Primers for the Cre allele and for Runx1 wild-type and floxed alleles have been described previously . The following primers were used for the Tau wild-type allele, 5'-AAT GTC ACC TGC TTT AGT GGG-3' and 5'-TGG GAA GGT GAA TAT TCA ACC-3'; and for the Neo-containing Tau floxed allele, 5'-GAT CGG CCA TTG AAC AAG ATG GAT TGC-3' and 5'-AGC TCT TCA GCA ATA TCA CGG GTA GCC-3'. All animal handling, surgeries, and behavioral test protocols (described below) were approved by the Institutional Animal Care and Use Committee at Dana-Farber Cancer Institute.
Real-time RT-PCR analysis of gene expression
Two biologically duplicated sets of total RNA were isolated from lumbar DRG of adult Runx1 F/F , Runx1 F/F ;Wnt1 Cre , or Runx1 F/F ;Nav1.8 Cre mice using TRIZOL (invitrogen, USA) and following the manufacturer's protocol. Reverse transcription was performed with 2.5 μg of RNA by using Superscript III first strand synthesis kit (Invitrogen, USA). Real-time quantitative PCR was then performed using SYBR green master mix (Invitrogen, USA) in a 7500 Real-time PCR machine (Applied Biosystems, USA). The following primer pairs were used: TRPA1 (Forward: 5'-GGAGACCCTGCTTCACAGAG-3', Reverse: 5'-AGTGGAGAGCGTCCTTCAGA-3'), HPRT (Hypoxanthine phosphoribosyl transferase) (Forward: 5'-GGCCAGACTTTGTTGGATTTG-3', Reverse: 5'-TGCGCTCATCTTAGGCTTTGT-3')
In Situ Hybridization and Immunostaining
Tissue preparation, the in situ hybridization (ISH) procedure, and the probes for TRPM8, TRPV1, Mrgprc11, CGRP, Mrgprd, TRPC3, P2X3, Mrgpra3, Mrgprb4, TrkA, SCG10, DRASIC, Ret, and GluR5/Grik1, have been described previously [14, 24, 41]. The probes for PKCq and Mrgprb5 were amplified with gene specific sets of PCR primers from cDNA template from adult mouse DRG. Immunohistochemistry (IHC) using rabbit anti-Runx1 (T. Jessell, Columbia University), rabbit anti-TrkA (L. Reichardt, UCSF) or IB4-biotin (10 μg/ml, Sigma) was carried out as previously described . IHC using rabbit anti-TRPV1 (1/4000, AbCam, USA) was performed on floating sections as described previously . ISH combined with rabbit anti-Runx1, rabbit anti-CGRP (Peninsula, USA) or IB4-biotin staining has been previously described . Fluorescent immunostaining images were photographed first, followed by the development of the ISH signals. The brightfield images of ISH signals were inverted and then merged with the fluorescence images. This sequential photographing avoids the masking of low-level fluorescent signals by non-fluorescent in situ signals, leading to a more sensitive detection of the coexpression of Runx1, CGRP, or IB4 with genes of interest. For GFP/ISH double stainings, GFP fluorescent images were directly photographed on sections (in RNase-free PBS solution) and then ISH was carried out as described above. The brightfield images of ISH signals were inverted, and then merged with the fluorescence images.
To count DRG neurons, L4/L5 lumbar DRG were dissected from two to three pairs of mutant and control mice, sectioned in a series of eight slides at a 12 μm thickness, and each set processed for immunostaining or used for ISH . The number of neurons per set of sections was reported. Only cells containing nuclei and showing levels of expression clearly above background were counted. At least three independent L4 or L5 lumbar DRG were used for each counting. Averages and standard errors of the mean (SEM) were calculated, and the difference between wild-type and mutant samples was subjected to a Student's t test (Two-Sample Assuming Unequal Variance), with p < 0.05 considered significant.
The spared nerve injury (SNI) model for neuropathic pain was performed on adult mice (P60 to P90) as described for rats . The animals used were Runx1 F/F control and Runx1 F/F ;Nav1.8 Cre mutant mice or Tau-Runx1 F control and Tau-Runx F ;Nav1.8 Cre gain-of-function mutant mice. Briefly, animals were anesthetized with an IP injection of 30 μl of nembutal sodium solution (50 mg/ml, Ovation) or by exposure to isofluorane (2%). An incision was made on the lateral mid-thigh, and the underlying muscle was separated to expose the sciatic nerve. The three branches of the sciatic nerve (tibial, common peroneal, and sural nerves) were carefully separated while minimizing any contact with or stretching of the sural. The tibial and common peroneal nerves were then individually ligated with 6.0 silk sutures and cut distally. 2-3 mm distal to the ligation of each of the tibial and common peroneal nerves were removed. The muscle and skin incisions were closed with silk sutures (6.0). For CFA-mediated inflammation, mice were briefly anesthetized with isofluorane (3-5 min at 2%), and 15 μl of Complete Freund's Adjuvant (CFA) (Sigma) were injected into the plantar surface of the left hindpaw. The thickness of the feet, before and two days after CFA injection, was measured to examine edema development. In 9 Runx1 F/F control mice and 14 Runx1 F/F ;Nav1.8 Cre mutant mice, the thickness of the feet increased similarly from 2.31 ± 0.06 mm to 2.90 ± 0.09 mm (t-test, p < 0.001), and from 2.24 ± 0.09 mm to 2.99 ± 0.07 mm (t-test, p < 0.001), respectively. There was no significant difference in the thickness of CFA-injected paws between these two genotypes (t-test, p > 0.05). In 15 Tau-Runx1 F control mice and 11 Tau-Runx1 F ;Nav1.8 Cre mutant mice, the thickness of the feet increased similarly from 2.61 ± 0.02 mm to 3.24 ± 0.05 mm (t-test, p < 0.01), and from 2.36 ± 0.09 mm to 3.10 ± 0.11 mm (t-test, p < 0.001), respectively. There was no significant difference in the thickness of CFA-injected paws between these two genotypes (t-test, p > 0.05). For NGF-mediated inflammation, 10 μl of NGF (Sigma, USA) diluted in saline at 5 ng/μl was injected into the plantar surface of the left hindpaw.
All animals were acclimatized to the behavioral testing apparatus in three to five 'habituation' sessions. After habituation, two baseline measures were recorded on two consecutive days for each of the behavioral tests prior to the surgery. After the surgical procedures (considered as day 0), the behavioral tests were performed at defined intervals (see Figs. 3 and 5). The experimenter was blinded to the genotype of the animals. To measure mechanical pain, we placed the animals on an elevated wire grid and the lateral plantar surface of the hindpaw was stimulated with calibrated Von Frey monofilaments (0.008-2 g). We started with the 0.16 g filament and moved up if response is negative and down if response is positive. The withdrawal threshold for the Von Frey assay was determined as the filament at which the animal withdrew, flicked or licked its paw at least twice in ten applications. To measure surface heat pain, we placed mice on a hot plate (IITC, USA) and the latency to hindpaw flicking, licking, or jumping was measured. The hot plate was set at 50°C, and all animals were tested sequentially with at least 5 min between tests. A cutoff time of 60 seconds was set for testing at 50°C. To measure radiant heat pain, animals were put in plastic boxes and the plantar paw surface was exposed to a beam of radiant heat (IITC, USA) according to the Hargreaves method . Paw withdrawal latency was then recorded (beam intensity was adjusted to result in a latency of 8-12 seconds for control animals baselines). The heat stimulation was repeated 3 times at an interval of 5-10 min for each animal and the mean calculated. A cutoff time of 18 seconds was set to prevent tissue damage.
Baseline data were calculated as the average of two independent tests performed on two consecutive days. Post-CFA data were taken from a single test performed two days post-injection and subjected to the Student's t-test (Two-Sample Assuming Unequal Variance). Post-SNI Von Frey time course measurements for Runx1 F/F compared to Runx1 F/F ;Nav1.8 Cre and Tau-Runx1 F compared to Tau-Runx1 F ;SNS Cre were analyzed by two-way repeated ANOVA (R, R Development Core Team, Austria) followed by Bonferroni's posttest. Von Frey results in SNI animals were plotted using a log scale. p < 0.05 was accepted as statistically significant. Post-NGF time course measurements (Von Frey and Hargreaves) for Tau-Runx1 F compared to Tau-Runx1 F ;SNS Cre were analyzed by two-way repeated ANOVA (R, R Development Core Team, Austria) followed by Bonferroni's posttest.
We thank Drs. Gary Gilliland and Nancy Speck for the Runx1 conditional knockout mice, Dr. Rohini Kuner for the Nav1.8 Cre mice, and Dr. Tom Jessell for the Runx1 antibody. We thank Drs. Clifford Woolf, Tarek Samad, Ru-Rong Ji, and Gary J. Brenner for technical assistance in performing surgeries and measuring pain behaviors and for their critical reading of this manuscript. The work is supported by the NIH training grant to FY (NINDS: 5T32NS007473-09), and the NIH grants from NIDCR (1R01DE018025) and NINDS (5P01NS047572) to QM. QM is a Claudia Adams Barr Scholar.
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