Neuropeptide deficient mice have attenuated nociceptive, vascular, and inflammatory changes in a tibia fracture model of complex regional pain syndrome
© Guo et al.; licensee BioMed Central Ltd. 2012
Received: 26 August 2012
Accepted: 21 November 2012
Published: 28 November 2012
Distal limb fracture in man can induce a complex regional pain syndrome (CRPS) with pain, warmth, edema, and cutaneous inflammation. In the present study substance P (SP, Tac1−/−) and CGRP receptor (RAMP1−/−) deficient mice were used to investigate the contribution of neuropeptide signaling to CRPS-like changes in a tibia fracture mouse model. Wildtype, Tac1−/−, and RAMP1−/− mice underwent tibia fracture and casting for 3 weeks, then the cast was removed and hindpaw mechanical allodynia, unweighting, warmth, and edema were tested over time. Hindpaw skin was collected at 3 weeks post-fracture for immunoassay and femurs were collected for micro-CT analysis.
Wildtype mice developed hindpaw allodynia, unweighting, warmth, and edema at 3 weeks post-fracture, but in the Tac1−/− fracture mice allodynia and unweighting were attenuated and there was no warmth and edema. RAMP1−/− fracture mice had a similar presentation, except there was no reduction in hindpaw edema. Hindpaw skin TNFα, IL-1β, IL-6 and NGF levels were up-regulated in wildtype fracture mice at 3 weeks post-fracture, but in the Tac1−/− and RAMP1−/− fracture mice only IL-6 was increased. The epidermal keratinocytes were the cellular source for these inflammatory mediators. An IL-6 receptor antagonist partially reversed post-fracture pain behaviors in wildtype mice.
In conclusion, both SP and CGRP are critical neuropeptide mediators for the pain behaviors, vascular abnormalities, and up-regulated innate immune responses observed in the fracture hindlimb. We postulate that the residual pain behaviors observed in the Tac1−/− and RAMP1−/− fracture mice are attributable to the increased IL-6 levels observed in the hindpaw skin after fracture.
Complex regional pain syndrome (CRPS) usually develops after a regional injury and presents with distal limb nociceptive, vascular, and bone changes that exceed the expected clinical course of the inciting injury in both magnitude and duration, frequently resulting in significant motor impairment and disability. CRPS symptoms usually gradually improve over the first year after injury, but persistent CRPS is a serious problem, resulting in edema, pain, weakness, contractures, and bone loss. Over 80% of chronic CRPS patients are severely disabled . The debate regarding the underlying mechanisms of CRPS has been dynamic and controversial, and despite extensive investigation, the pathophysiology of this condition remains undefined and there is considerable disagreement on whether any treatment for CRPS is effective [2–5]. There is clearly a need for mechanism based effective and safe treatments for this debilitating complication of injury.
Early CRPS clinical findings include vasodilatation, redness, warmth, increased spontaneous protein extravasation, edema, periarticular bone loss, pain, and allodynia in the injured limb . Population-based studies indicate that distal limb fracture is the most common cause of CRPS [7, 8] and we have developed and characterized a rat fracture CRPS model mimicking the clinical condition. After distal tibia fracture and cast immobilization for 4 weeks the rats develop chronic unilateral hindlimb warmth, edema, facilitated spontaneous protein extravasation, periarticular osteoporosis, hindlimb unweighting, and allodynia, changes paralleling those observed in CRPS patients . The vascular and nociceptive changes characteristic of the early stages of CRPS suggest an inflammatory process, yet there is little evidence for an immunocyte response in these patients [10–15]. Similarly, in the rat CRPS model we observe no evidence of macrophage, T-cell, or neutrophil infiltration in the hindpaw skin at 4 weeks post-fracture . On the other hand, there is compelling evidence that neurogenic inflammatory responses are exaggerated in the CRPS limb and in the rat fracture model.
Neurogenic inflammation is mediated by the depolarization of small sensory afferents in the skin, triggering the release of substance P (SP) and calcitonin gene-related peptide (CGRP), neurotransmitters that activate their receptors in the dermal vasculature to induce protein extravasation and vasodilatation. Electrically evoked extravasation and vasodilatation responses are enhanced in CRPS patients and when SP is microdialyzed in the skin there is exaggerated protein extravasation, direct evidence of facilitated neurogenic inflammatory responses in CRPS [17, 18]. Furthermore, serum levels of SP and CGRP are elevated in CRPS patients [19–21]. Similarly, after tibia fracture in rats there is increased SP and CGRP expression in the sciatic nerve and in serum, up-regulated SP NK1 receptor expression in the endothelial cells and keratinocytes of the hindpaw skin, and SP evoked extravasation and edema responses are enhanced in the fracture hindlimb . Treating fracture rats with an SP NK1 receptor antagonist reduced hindlimb allodynia, warmth, and edema, suggesting that exaggerated SP signaling contributes to the development of CRPS-like changes after fracture .
Inflammatory cytokine levels are up-regulated in CRPS skin [23–25], and levels of interleukin 1b (IL-1b), interleukin 6 (IL-6), tumor necrosis factor a (TNFa), and nerve growth factor (NGF) are elevated in the hindpaw skin keratinocytes of fracture rats [16, 26–28]. Treating fracture rats with the TNF inhibitor etanercept, the IL-1 receptor antagonist anakinra, or the anti-NGF antibody tanezumab reduced hindpaw allodynia and unweighting at 4 weeks post-fracture [26, 27, 29].
Collectively, these data support the hypothesis that fracture causes exaggerated SP and CGRP signaling in the skin, resulting in increased extravasation, edema, warmth, and pain behavior. The aims of current study were to develop a tibia fracture CRPS model in mice and utilize this model in SP deficient (Tac1−/−) and in CGRP receptor (RAMP1−/−) deficient mice to directly test the hypothesis that neuropeptide signaling is required for the development of the post-fracture innate immunity responses contributing to the development of pain behavior in the fracture model.
Characterizing the WT, Tac1−/−, and RAMP1−/− mice
Nociceptive and vascular changes after fracture
Figures 2A and E illustrate that after cast removal both the WT and the transgenic mice had developed reduced withdrawal thresholds to von Frey testing, but the Tac1−/− and RAMP1−/− mice had significantly less allodynia than WT fracture mice. Similarly, at 3 weeks post-fracture both the WT and the transgenic mice unweighted the injured hindlimb, but both the Tac1−/− and RAMP1−/− mice had significantly less hindpaw unweighting than the WT mice (Figures 2B,F).
The WT mice also exhibited increased hindpaw temperature and thickness at 3 weeks post-fracture, vs the contralateral paw, but the SP deficient mice failed to develop significant hindpaw warmth or edema (Figures 2C,D). The RAMP1−/− fracture mice failed to develop hindpaw warmth after fracture, but had a similar degree of hindpaw edema as the WT fracture mice (Figures 2G,H).
Effects of fracture on hindpaw skin cytokine and NGF levels
Cytokine induction in hindpaw skin keratinocytes after tibia fracture
An IL-6 receptor antagonist reduced post-fracture pain behavior in WT mice
Tibia fracture effects on trabecular and cortical bone microarchitecture
μCT skeletal morphology of the ipsilateral femurs in the control and tibia fractured (FX) wild type and SP deficient mice at 3 weeks post-injury
SP deficient mice
22.8 ± 0.7
13.4 ± 0.8
22.3 ± 1.3
13.9 ± 0.5
5.1 ± 0.1
4.6 ± 0.1
5.3 ± 0.1
4.7 ± 0.1
58.5 ± 0.5
47.6 ± 0.7
54.7 ± 1.6
46.6 ± 0.5
185 ± 3
209 ± 5
177 ± 4
203 ± 3
156 ± 7
106 ± 8
181 ± 6
125 ± 5
1.04 ± 0.01
0.90 ± 0.02
0.97 ± 0.04
0.85 ± 0.02
2.22 ± 0.04
2.22 ± 0.06
2.11 ± 0.07
2.12 ± 0.05
1.18 ± 0.03
1.32 ± 0.06
1.14 ± 0.04
1.27 ± 0.04
224 ± 2
189 ± 5
211 ± 5
185 ± 4
9.9 ± 0.1
10.3 ± 0.2
9.8 ± 0.2
10.0 ± 0.1
47.0 ± 0.4
40.7 ± 1.1
45.9 ± 0.4
40.3 ± 0.8
In this study we describe a novel mouse fracture model that resembles CRPS in; 1) traumatic etiology and natural history, 2) vascular changes, 3) mechanical sensitivity and guarding, 4) up-regulation of cutaneous inflammatory mediators, and 5) regional periarticular bone loss. After a tibia fracture and 3 weeks cast immobilization the WT mice developed chronic allodynia, unweighting, warmth, and edema (Figure 2), and these vascular and nociceptive changes gradually resolved over a period of 16 weeks (Figure 3).
In an earlier investigation we tested the hypothesis that exaggerated neurogenic inflammatory responses contribute to the vascular and nociceptive sequelae of fracture in rats. When fracture rats were treated with a single-dose of an SP NK1 receptor antagonist there was a 51% reduction in hindpaw spontaneous protein extravasation, a 56% decrease in edema, and a 40% reduction in von Frey fiber allodynia . The results of the current study confirm and extend these findings. SP deficient (Tac1−/−) fracture mice had 54% less von Frey allodynia, 70% less hindpaw unweighting, and no hindpaw warmth or edema, compared to WT fracture mice at 3 weeks post-injury (Figure 2). Time-course studies demonstrated further differences in the magnitude and duration of post-fracture nociceptive behaviors in the WT and Tac1−/− mice. The WT mice developed nociceptive behaviors persisting for 8–15 weeks after fracture, while in the SP deficient mice nociceptive behaviors persisted for only 4–6 weeks post-fracture (Figure 3). These data confirm that SP is a critical regulator for the development of nociceptive and vascular sequelae after fracture. Similarly, CGRP receptor deficient (RAMP1−/−) fracture mice had attenuated allodynia and hindpaw unweighting, and no hindpaw warmth, but post-fracture edema was not affected by the loss of CGRP signaling (Figure 2). The contribution of SP and CGRP signaling to post-fracture hindpaw warmth and the essential role of SP signaling in the development of post-fracture hindpaw edema correlates with the results of intradermal microdialysis studies in human subjects demonstrating that SP or CGRP perfusion induces a vasodilation response in the skin, but only SP perfusion can evoke a protein extravasation response . In addition, the sciatic nerve electrically evoked protein extravasation response was dramatically reduced in Tac1−/− mice and unchanged in the RAMP1−/− mice (Figure 1C), indicating that CGRP signaling does not contribute to neurogenic extravasation in mice. Spontaneous protein extravasation is up-regulated in CRPS affected limbs , leading to increased interstitial fluid flow and distal limb edema, an important component of CRPS .
When SP or CGRP is microdialyzed through the skin of normal volunteers  or in CRPS skin  there is no immediate painful response, leading us to postulate that SP and CGRP act as intermediate mediators in the development of post-traumatic pain. Increased levels of the inflammatory cytokines TNFα and IL-6 are observed in experimental blister fluid or skin biopsies from the affected, but not the contralateral limbs of CRPS patients [23–25]. TNFα, IL-1b, IL-6, and NGF are up-regulated in the hindpaw skin at 4 weeks after tibia fracture in rats and fracture rats treated systemically with the TNF inhibitor etanercept, the IL-1 receptor antagonist anakinra, or the NGF antibody tanezumab had reduced allodynia and hindlimb unweighting, indicating an important role for cytokine and growth factor signaling in the development of trauma induced chronic pain [26, 27, 29].
The TNF, IL-1, and NGF inhibitors we have tested in the fracture rat model are large molecules that can’t cross the blood-brain barrier, suggesting that the pronociceptive effects of these inflammatory mediators probably occur at the nociceptor level in the sensitized hindpaw skin. Pro-inflammatory cytokines and NGF can immediately evoke spontaneous firing and sensitization in primary sensory afferents [32–34]. Furthermore, we have observed that intraplantar injection of these inflammatory mediators into normal hindpaw skin rapidly induces nociceptive sensitization and have identified keratinocytes as the primary cellular source of these inflammatory mediators in the fracture hindpaw (Figure 5) . Robust keratinocyte proliferation and epidermal thickening was also observed in the fracture hindpaw . Collectively, these data indicate that up-regulated keratinocyte proliferation and/or inflammatory mediator expression results in increased cutaneous inflammatory mediator levels in the fracture hindpaw, with subsequent nociceptive sensitization.
Neurotransmitter release evoked by capsaicin injection or electrical nerve stimulation stimulates the production of TNFα, IL-1β, IL-6, and NGF in the skin of rats, but it is unknown which specific transmitters mediate these inflammatory effects [35, 36]. Previous studies have observed that SP and CGRP signaling can stimulate the secretion of IL-1β and NGF from keratinocyte cell cultures, in vitro evidence that these neuropeptides can directly stimulate keratinocyte inflammatory mediator release [37–39].
Figure 4 illustrates the robust increase in TNFα, IL-1β, IL-6, and NGF protein levels observed in the hindpaw skin at 3 weeks post-fracture in the WT mice. Cytokines are normally expressed transiently in response to injury and infection and the persistent regional elevation of cytokines observed at 3 weeks post-fracture indicates ongoing cytokine synthesis by the keratinocytes (Figure 5), which we postulate is the result of exaggerated neuropeptide signaling. In support of this hypothesis, Tac1 or RAMP1 gene deletion prevented the up-regulation of TNFα, IL-1β, and NGF in the injured hindlimb (Figure 4). There were no differences between the WT, Tac1−/− and RAMP1−/− mice in baseline cutaneous cytokine and NGF levels, evidence that the low basal levels of cytokines and NGF expressed in normal skin are not dependent on neuropeptide signal.
Both the Tac1−/− and RAMP1−/− fracture mice had increased IL-6 levels in the hindpaw skin, and there was residual, albeit attenuated, allodynia and unweighting in these transgenic fracture mice compared to WT fracture mice (Figures 2,4). We postulated that IL-6 signaling contributes to post-fracture allodynia and unweighting and that the increase in skin IL-6 levels after fracture in the Tac−/− and RAMP1−/− mice is the mechanism for the residual post-fracture allodynia and hindpaw unweighting observed in these strains. To test this hypothesis we treated WT fracture mice with an IL-6 receptor antagonist (TB-2-081), and this treatment partially reversed fracture-induced allodynia and unweighting (Figure 6), supporting the hypothesis that increased cutaneous IL-6 signal contributes to allodynia and unweighting after fracture.
This study provides the first in vivo evidence that SP and CGRP act as intermediate mediators in the post-traumatic inflammatory cascade by chronically up-regulating inflammatory proteins that can directly sensitize cutaneous nociceptors. In contrast to other inflammatory mediators examined in this study, the post-fracture up-regulation of IL-6 did not require CGRP signaling and was only partially dependent on SP signaling. The significance of IL-6 signaling was confirmed by antagonist studies demonstrating its role in the maintenance of post-fracture nociceptive behavior. Collectively, these data suggest that neuronal regulation of innate immunity can mediate inflammatory changes observed in CRPS skin.
After tibia fracture WT mice developed trabecular, and to a lesser extent cortical bone loss in the ipsilateral femur when compared to unfractured controls (Figure 7, Table 1). These data are consistent with the results observed in the rat tibia fracture model , as well as in CRPS patients who develop periarticular bone loss after fracture . The net effects of SP signaling on bone acquisition and resorption in vivo are unknown, but previously we observed that the SP NK1 receptor is expressed in both bone marrow stromal cells and bone marrow macrophages and SP signaling stimulates bone marrow stromal cell osteogenic activity as well as macrophage osteoclast differentiation and resorption activity in vitro . The WT and SP deficient (Tac1−/−) mice had similar baseline bone parameters and no post-fracture differences were observed in skeletal integrity between groups, indicating that SP signaling does not regulate post-traumatic bone loss in mice (Figure 7 and Table 1).
CRPS patients usually have deep tissue pain, primarily tenderness over the distal limb joints and pain with range of motion [15, 42]. It is unknown whether the neuroinflammatory changes observed in the fracture limb skin may also be occurring in the joints or muscles of the injured limb, but there are some intriguing clues. The most prominent histological finding in CRPS synovial biopsies is the proliferation of synovial lining cells . Synoviocytes express NK1 receptors and very low concentrations of SP can stimulate synoviocyte proliferation in vitro [43, 44]. Stimulated synoviocytes express IL-6 and CCL2, a chemokine which induces monocytes to leave the bloodstream and enter the surrounding tissue to become synovial macrophages, the primary source of TNFα and IL-1β in rheumatoid joints . Examining neuroinflammatory articular changes after fracture could potentially identify signaling mechanisms contributing to the articular tenderness and joint motion pain associated with CRPS.
There have been numerous unsuccessful clinical trials testing the analgesic efficacy of NK1 antagonists in post-herpetic pain, diabetic neuropathy, osteoarthritis, and migraine patients . Only post-operative pain has been responsive to NK1 antagonist analgesia in clinical trials  and our previous work with the hindpaw incision post-operative pain model in SP deficient mice  suggests that SP signaling contributes to both the post-incision increase in inflammatory mediator levels in the traumatized skin and the subsequent post-operative pain. We postulate that the mixed results of the NK1 antagonist clinical trial data reflect differing pathophysiologic mechanisms underlying the various pain etiologies examined in these trials. CGRP receptor antagonists have been demonstrated to effectively reduce pain in migraine patients but have not been tested in other types of pain patients . The results of the current study supports the hypothesis that SP and CGRP signaling contribute to the post-fracture up-regulation of cutaneous inflammatory mediators capable of inducing pain behaviors in the mouse CRPS model and these data provide a translational basis for future clinical trials with NK1 or CGRP antagonists in early CRPS.
Baseline determinations were made of bilateral hindpaw von Frey withdrawal thresholds, weight bearing, temperature, and thickness in the wildtype (WT), SP deficient (Tac1−/−), and CGRP receptor deficient (RAMP1−/−) mice. Then the mice underwent a right distal tibia fracture with cast immobilization for 3 weeks, then the cast was removed and the mice were retested the following day. One group of WT mice did not undergo fracture and served as controls. Two groups of additional groups of control mice were used in these experiments; 1) WT mice that had a crush injury (no fracture or cast) to the distal tibia using the same hemostat used to fracture the tibia, and 2) WT mice that underwent hindlimb cast immobilization for 3 weeks with no fracture. After behavioral testing the mice were euthanized and the bilateral hindpaw skin and femurs were collected. Another group of WT fracture mice underwent behavioral testing the day after cast removal and the next day were subcutaneously injected with either the IL-6 receptor antagonist TB-2-081  or vehicle (50% PBS/50%EtoH, total volume 50 ul) and 15 min later behavioral testing was repeated. Another group of WT and Tac1−/− fracture mice underwent long-term behavioral testing over an 18-week period after cast removal. Electrically evoked protein extravasation responses were evaluated in unfractured WT, Tac1−/−, and RAMP1−/− control mice to confirm our hypothesis that the SP deficient mice had impaired neurogenic extravasation and that RAMP−/− mice had normal extravasation responses.
Mice that were homozygous for a disruption of the SP expressing Tac1 (Tac1−/−) gene (B6.Cg-Tac1tm1Bbm/J) and wildtype (WT) controls (C57Bl/6) were obtained from Jackson Laboratory. CGRP receptor binding requires the receptor activity-modifying protein 1 (RAMP1). Mice that were homozygous for disruption of the RAMP1 gene (RAMP1−/−) were generated by Dr Kazutake Tsujikawa (Dept Immunology, Osaka University) on a C57Bl/6 background . The Cre-loxP system was used to delete exon 2 of the RAMP1 gene, with splicing of exons 1 to 3 creating a frameshift resulting in a termination codon at the beginning of exon 3.
The genotype of each breeding pair was confirmed by extracting DNA from tail snips and performing a PCR assay using an Extract-N-AmpTM kit (XNAT-1KT, Sigma). Forward (oIMR2033, 5′-GCT CAT CAG TAT GTG ACA TAG AAA) and reverse (oIMR2032, 5′-AGA ATT TAA AGC TCT TTT GCC) primers were used to identify the Tac1−/− mice, based on the Jackson Laboratory’s genotyping protocol. The Tac1−/− mice yield shorter PCR product (size 130 bp) on a 3% agrose gel, whereas WT mice yielded longer product (180 bp). Genotyping for the RAMP1−/− mice used a 5′-specific primer upstream of the 5′ site in intron 1 (5′-CAGAATGGAGAAACTGAGTAGAGC-3′), a 3′-specific primer immediately downstream of the first loxP site in intron 1 (5′-AGGAAGGAACGTAACACAGGTGG-3′), and a 3′-specific primer downstream to the last loxP site (5′-GCTGTGCGTGGTGATGGAGG-3′). The PCR reaction conditions were: 40 cycles of a reaction consisting of 30 s of denaturation at 94°C, 30 s of annealing at 59°C, and 60 s of elongation at 72°C . Wildtype and RAMP1−/− mice yielded PCR products of 108 and 240 bp, respectively.
The Tac1−/− mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities . SP is not immunodetectable in the Tac1−/− mice and these mice have slightly reduced nociceptive responses to moderate/intense pain stimuli . The RAMP1−/− mice are also viable, fertile, normal in size, and do no display any gross physical abnormalities . The RAMP1−/− mice have hypertension without tachycardia, exhibit no vasodilation response to intravenous CGRP, and are incapable of nurturing their new born pups, thus requiring surrogate mothers to raise their litters . All experiments were done with the approval of our Institutional Animal Care and Use Committee.
The fracture model was performed in 3 month-old male mice. Under isoflurane anesthesia a hemostat was used to make a closed fracture of the right tibia just distal to the middle of the tibia. Then the hindlimb was wrapped in casting tape (Delta-Lite) so the hip, knee and ankle were all fixed. The cast extended from the metatarsals of the hindpaw up to a spica formed around the abdomen. A window was left open over the dorsal paw and ankle to prevent constriction when post-fracture edema developed. After fracture and casting, the mice were given subcutaneously 2 days of Buprenorphine (0.05 mg/kg) and Baytril (5 mg/kg) as well as 1.5 ml of normal saline. At 3 weeks after surgery the mice were anesthetized with isoflurane and the cast removed. All mice had union at the fracture site by manual inspection.
Hindpaw hyperalgesia and unweighting
Mechanical allodynia was assayed using nylon von Frey filaments according to the “up-down” algorithm as previously described . The mice were placed on wire mesh platforms in clear cylindrical plastic enclosures 10 cm in diameter and 40 cm in height, and after 15 minutes of acclimation von Frey fibers of sequentially increasing stiffness were applied against the hindpaw plantar skin at approximately midsole, taking care to avoid the tori pads, and pressed upward to cause a slight bend in the fiber and left in place for 5 sec. Withdrawal of or licking the hindpaw after fiber application was scored as a response. When no response was obtained the next stiffest fiber in the series was applied to the same paw; if a response was obtained a less stiff fiber was applied. Testing proceeded in this manner until 4 fibers had been applied after “negative + positive or positive + negative” response. Estimation of the mechanical withdrawal threshold by data fitting algorithm permitted the use of parametric statistics for analysis . Baseline von Frey withdrawal thresholds were identical in the WT, Tac1−/−, and the RAMP1−/− mice.
An incapacitance device (IITC Inc) was used to measure hindpaw unweighting. The mice were manually held in a vertical position over the apparatus with the hindpaws resting on separate metal scale plates and the entire weight of the mouse was supported on the hindpaws. The duration of each measurement was 6 s and 6 consecutive measurements were taken at 10 s intervals. All 6 readings were averaged to calculate the bilateral hindpaw weight-bearing values.
A laser sensor technique was used to determine the dorsal–ventral thickness of the hindpaw, as we have previously described . The measurement sensor device used in these experiments (Limab) has a measurement range of 200 mm with a 0.01 mm resolution.
The temperature of the hindpaw was measured using a fine wire thermocouple (Omega) applied to the paw skin, as previously described previously . The investigator held the wire using an insulating Styrofoam block. Three sites were tested over the dorsum of the hindpaw: the space between the first and second metatarsals (medial), the second and third metatarsals (central), and the fourth and fifth metatarsals (lateral). After a site was tested in one hindpaw the same site was immediately tested in the contralateral hindpaw. The testing protocol was medial dorsum right then left, central dorsum right then left, lateral dorsum right then left, medial dorsum left then right, central dorsum left then right, and lateral dorsum left then right. The six measurements for each hindpaw were averaged for the mean temperature.
Enzyme immunoassay for TNFα, IL-1β, IL-6 and NGF levels in hindpaw skin
The mice were deeply anesthetized and bilateral dorsal and plantar hindpaw skin were collected rapidly and placed into ice cold 0.9% NaCl containing a cocktail of protease inhibitors (Complete™, Roche Applied Science), homogenized, then centrifuged (10 min, 12,000 G, 4°C), then supernatant fractions were frozen at −80°C. An aliquot was subjected to protein assay (DC Protein Assay, Bio-Rad) to normalize mediator levels. Cytokine protein levels were determined using a custom Bio-Plex kit for TNFα, IL-1β, and IL-6, analyzed on a Bio-Plex system array reader (Bio-Rad) as previously described . Samples were diluted 1:2 prior to analysis in the buffer supplied, and all samples were run in duplicate for each assay. We demonstrated previously that the dynamic range of sensitivity of this assay was sufficient to measure both baseline and incision-stimulated levels of the chosen cytokines . Standard curves for each of the analyzed substances were included in each run, and sample concentrations were calculated using Bio-Plex Manager software. NGF levels were determined using the ChemiKine EIA kit (Chemicon). Sample preparation was identical to that described for the cytokines.
Western blot assay for RAMP1 protein levels in hindpaw skin
Western blotting was carried out to confirm RAMP1 protein knockout in the RAMP1−/− mice. Hindpaw skin of WT and RAMP1−/− mice was collected under isoflurane anesthesia and homogenized in modified RIPA buffer (50 mM Tris–HCl, 150 mM NaCl, 1 mMEDTA, 1% Igepal CA-630, 0.1% SDS, 50 mM NaF, and 1 mM NaVO3) containing protease inhibitors (aprotinin (2 μg/ml), leupeptin (5 μg/ml), pepstatin (0.7 μg/ml), and PMSF (2 mM); Sigma). The homogenate was centrifuged at 13,000 g for 30 min at 4°C. Total protein concentration of the homogenate was measured using a Coomassie Blue Protein Assay (Bio-Rad) and BSA protein standard (Pierce). The supernatant was subjected to western blot analysis. Equal amounts of protein (50 μg) were subjected to SDS-PAGE (12% Tris–HCl acrylamide gel, Bio-Rad) and electrotransferred onto a polyvinylidene difluorided membrane (Millipore). The blots were blocked with 5% non-fat dry milk in tris-buffered saline, incubated with goat anti-RAMP1 antibody (Novus Biologicals) overnight at 4°C and further incubated with HRP-conjugated secondary antibody (Santa Cruz Biotechnology) for 1 hr at room temperature. After washing in TBST three times, the blot was then incubated in ECL plus chemoluminescence reagents (Amersham) and scanned by PhosphoImager (Typhoon, GE Healthcare) to detect specific bands.
Immunofluorescence confocal microscopy
The mice were euthanized and perfused with 50 ml 4% paraformaldehyde (PFA) in phosphate buffered saline, pH 7.4, via the ascending aorta; the plantar hindpaw skin including sub-dermal layers was removed and post-fixed in 4% PFA for 2 hours, then the tissues were treated with 30% sucrose in PBS at 4°C before embedding in OCT. Following embedding, 8-μm thick slices were made using a cryostat, mounted onto Superfrost microscope slides (Fisher Scientific), and stored at −80°C. Frozen sections were permeabilized and blocked with PBS containing 10% donkey serum and 0.3% Triton X-100, followed by exposure to the primary antibodies overnight at 4°C in PBS containing 2% serum. Upon detection of the first antigen, primary antibody from a different species against the second antigen was applied to the sections and visualized using an alternative fluorophore-conjugated secondary antibody. Sections were then rinsed in PBS and incubated with fluorophore-conjugated secondary antibodies against the immunoglobulin of the species from which the primary antibody was generated. After three washes, the sections were mounted with anti-fade mounting medium (Invitrogen). Images were obtained using a confocal microscope (Zeiss LSM/510 Upright 2 photon; Carl Zeiss) and stored on digital media.
For IL-6 immunostaining rabbit anti human IL-6 antibody (1:400, LifeSpan Biosciences Inc) was labeled with donkey anti-rabbit IgG (1:500) conjugated with Dylight 488 secondary antibodies (Jackson ImmunoResearch). Control experiments included incubation of slices in primary and secondary antibody-free solutions both of which led to low intensity non-specific staining patterns in preliminary experiments. For the detection of epidermal keratinocytes, rabbit anti keratin 14 polyclonal antibody (Convance Inc) was labeled with Alexa Fluor 555 microscale protein labeling kit (Invitrogen Molecular Probes) under optimum conditions according to the manufacture’s protocol, and quantified using a NanoDrop ND-1000 UV–vis spectrophotometer (NanoDrop Technologies), the concentration of purified protein conjugate was 0.34 mg/ml. Then an equal volume of glycerol (ACS grade) was added for a final concentration of 50%. Upon detection of the IL-6 as described above, 250 μl of a 1:200 dilution of the conjugate was used for the keratin 14 immunostaining. Control experiments included incubation of slices in primary and secondary antibody-free solutions both of which led to low intensity non-specific staining patterns in preliminary experiments.
For IL1-b immunostaining, the primary antibodies, rabbit anti-mouse IL-1b, 1:200 (Millipore) and mouse monoclonal antibody to Pan Keratin, 1:50 (Abcam) were used. Double labeling immunofluorescence was performed with donkey anti-mouse IgG (1:500) conjugated with Dylight 549, donkey anti-rabbit IgG (1:500) conjugated with Dylight 488 secondary antibodies (Jackson ImmunoResearch), incubated with respective primary antibodies. Control experiments included incubation of slices in primary and secondary antibody-free solutions both of which led to low intensity non-specific staining patterns in preliminary experiments.
Electrical stimulation-evoked extravasation procedure
Under isoflurane anesthesia both sciatic nerves were exposed in the thigh and tightly ligated proximal to the midthigh stimulation site. Then the incision was filled with warm mineral oil and a Plexiglas-platinum stimulating electrode was gently secured around the nerve (Harvard Apparatus). Evans blue dye (25 mg/kg, Sigma) dissolved in saline was administered through the jugular vein, and 10 minutes later bilateral sciatic nerves were stimulated for 5 min (5 Hz, 0.5-ms pulse duration, 10 mA) and then the mice were transcardially perfused with 30 ml normal saline. The plantar and dorsal skin on each hindpaw was excised and then placed in 1 ml of formamide in a shaker bath at 55°C for 72 h. The extracted dye concentration was determined with a spectrophotometer at 620 nm. Because Evans blue binds to serum albumin, the dye content of the hindpaw skin provides an accurate measure of electrically evoked protein extravasation into the interstitial space.
Microcomputed tomography (μCT)
To assess trabecular and cortical bone architecture, ex vivo scanning was performed using μCT (VivaCT 40, Scanco Medical AG) as we have previously described . In the distal femur, 160 transverse slices of 10.5 μm thickness (10.5-μm isotropic voxel size) encompassing a length of 1.68 mm were acquired, but only 100 slices encompassing 1 mm in the distal femur were evaluated, starting where the growth plate bridge across the middle of the metaphysis ends. The region of interest (ROI) was manually outlined on each CT slice, excluding the primary spongiosa, and extending distally from the growth plate. Image threshold was set by using an adaptive-iterative algorithm for trabecular bone. Scan parameters of threshold = 252, sigma = 0.8, support = 1 for proximal tibia were used to measure trabecular bone volume fraction (BV/TV, %), trabecular number (Tb.N, mm−1), trabecular thickness (Tb.Th, μm), trabecular separation (Tb.Sp, μm), and connectivity density (ConnD, mm−3) [56, 57].
For the mid-femur cortical bone evaluation, a total of 26 transverse CT slices were obtained at the mid-femur, each 21 μm thick totaling 0.546 mm in length (21 μm isotropic voxel size). Scan parameters of threshold = 350, sigma = 1.2, and support = 1 were used to measure total cross-sectional area (Tt.Ar, mm2), cortical bone area (B.Ar, mm2), cortical thickness (Ct.Th, μm), medullary area (Me.Ar, mm2), and bone perimeter (B.Pm, mm). Relative cortical bone area (B.Ar/T.Ar, %) was calculated.
A two-tailed Student’s t-test was used to analyze differences between groups. A two-way repeated measures ANOVA was used to analyze nociceptive and vascular behavioral data for differences between groups over time. All data were presented as the mean ± standard error of the mean, and differences were considered significant at a P value less than 0.05. Hindpaw temperature, thickness, and mechanical nociceptive thresholds data were analyzed as the difference between the fracture side (right) and the contralateral (left) side. Hindpaw weight bearing data was analyzed as a ratio between the right hindpaw weight and the sum of right and left hindpaws values ((2R/(R + L)) × 100%).
This study was supported by National Institutes of Health grants DK67197 and NS072168, and Department of Veterans Affairs, Rehabilitation Research and Development Merit grants F4516I and F7137R. A portion of this work was supported by the Intramural Research Programs of the National Institute on Alcohol Abuse and Alcoholism and the National Institute on Drug Abuse, National Institutes of Health.
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