Anoctamin 1 contributes to inflammatory and nerve-injury induced hypersensitivity
© Lee et al.; licensee BioMed Central Ltd. 2014
Received: 15 December 2013
Accepted: 20 January 2014
Published: 23 January 2014
Various pathological conditions such as inflammation or injury can evoke pain hypersensitivity. That represents the response to innocuous stimuli or exaggerated response to noxious stimuli. The molecular mechanism based on the pain hypersensitivity is associated with changes in many of ion channels in dorsal-root ganglion (DRG) neurons. Anoctamin 1 (ANO1/TMEM16A), a Ca2+ activated chloride channel is highly visible in small DRG neurons and responds to heat. Mice with an abolished function of ANO1 in DRG neurons demonstrated attenuated pain-like behaviors when exposed to noxious heat, suggesting a role in acute thermal nociception. In this study, we further examined the function of ANO1 in mediating inflammation- or injury-induced hyperalgesia or allodynia.
Using Advillin/Ano1 fl/fl (Adv/Ano1 fl/fl ) mice that have a functional ablation of Ano1 mainly in DRG neurons, we were able to determine its role in mediating thermal hyperalgesia and mechanical allodynia induced by inflammation or nerve injury. The thermal hyperalgesia and mechanical allodynia induced by carrageenan injection and spared-nerve injury were significantly reduced in Adv/Ano1 fl/fl mice. In addition, flinching or licking behavior after bradykinin or formalin injection was also significantly reduced in Adv/Ano1 fl/fl mice. Since pathological conditions augment nociceptive behaviors, we expected ANO1′s contribution to the excitability of DRG neurons. Indeed, the application of inflammatory mediators reduced the threshold for action potential (rheobase) or time for induction of the first action potential in DRG neurons isolated from control (Ano1 fl/fl ) mice. These parameters for neuronal excitability induced by inflammatory mediators were not changed in Adv/Ano1 fl/fl mice, suggesting an active contribution of ANO1 in augmenting the neuronal excitability.
In addition to ANO1's role in mediating acute thermal pain as a heat sensor, ANO1 is also capable of augmenting the excitability of DRG neurons under inflammatory or neuropathic conditions and thereby aggravates inflammation- or tissue injury-induced pathological pain.
Pain is one of many diverse sensations that humans experience in life. Pain is an unpleasant sensation that evokes suffering in humans. However, pain is also a warning sign for avoiding tissue injury from impending external noxious stimuli or aggravated pathological conditions. Acute pain that is mediated by noxious stimuli from external environments functions as a warning system that notifies the risk of immediate injury [1, 2]. In contrast, inflammation, nerve damage, or cancer causes pain hypersensitivity or exaggerated responses to non-noxious stimuli injury [1, 2]. Pain is initiated with the detection of noxious stimuli at terminals of DRG neurons innervated in peripheral nervous system. Nociceptor is a subclass of DRG neurons that is especially for transmitting nociceptive neural signals. Most of nociceptors are small-diameter and unmyelinated c-fibers . Nociceptors express many ion channels that are associated with pain signal generation. Many transient receptor potential (TRP) channels present in DRG neurons are involved in transducing noxious stimuli to neural signals [4, 5]. In addition to their role as a primary detector for noxious stimuli, these channels also play a role in transmitting nociceptive signals to the spinal cord, amplifying nociceptive neural signals. A good example is TRPV1, which is activated by diverse stimuli such as capsaicin, anandamide, lipoxygenase products, noxious heat or low pH [6–9]. TRPV1 is highly visible in nociceptors of DRG neurons and known to play an important role in transducing these noxious stimuli to nociceptive neural signals. Because Trpv1-/- mice failed to detect capsaicin-evoked pain and showed reduced response to heat, TRPV1 is now known to be a heat sensor that detects noxious heat . In addition, inflammation- and nerve injury-induced thermal hyperalgesia were also reduced in Trpv1-/- mice, suggesting a role for controlling transmission of nociceptive signals [9, 10]. Similarly, TRPA1 is activated by pungent chemicals or environmental irritants [11, 12]. TRPA1 is expressed in nociceptive neurons that are positive to TRPV1 or CGRP. In addition, bradykinin (BK), an algogenic substance released by tissue damage, stimulates TRPA1 . TRPA1 appears to contribute to cold hyperalgesia or allodynia after inflammation and nerve injury, because TRPA1 knock down evoked the reduction of cold hyperalgesia [13–15]. Thus, many channels in DRG neurons play a role in detecting noxious stimuli as primary sensors and are actively involved in modulating transmission of the nociceptive signals to the central nervous system.
ANO1 is a Cl- channel activated by intracellular Ca2+ [16–18]. ANO1 as a Ca2+-activated Cl- channel mediates transepithelial fluid secretion in the epithelia of many organs . Notably, ANO1 is implicated in salivation , mucin secretion in airway , intestinal function , and vascular tone . In addition, Yang et al. reported that ANO1 is expressed in DRG neurons, suggesting a role in somatosensation . A later study on the expression in DRG neurons revealed that ANO1 is highly co-localized with TRPV1, suggesting a role in nociception . Surprisingly, ANO1 is activated by heat over 44°C . Functional ablation of ANO1 in DRG neurons elicited significant loss of thermal pain . Although these results suggest that ANO1 plays a role in transducing noxious heat as a heat sensor, it is not known whether ANO1 is implicated in controlling excitability of sensory neurons, which mediates hyperalgesia or allodynia evoked by chronic tissue injury. Thus, we sought to determine ANO1′s roles in regulating hyperalgesia or allodynia evoked by pathological conditions such as inflammation or nerve injury.
This study was performed in accordance with protocols approved by the Committee on Laboratory Animals at Seoul National University. Experiments were also conducted according to the Ethical Guidelines of the International Association for the Study of Pain. We crossed Advcre/WT Ano1fl/fl homozygote with AdvWT/WT Ano1fl/fl homozygote, producing Advcre/WT Ano1fl/fl and AdvWT/WT Ano1fl/fl offsprings. Advcre/WT Ano1fl/fl mice were used as conditional knock-out mice (Adv/Ano1fl/fl) whereas AdvWT/WT Ano1fl/fl homozygote were used as a ‘control’ mice (Ano1fl/fl). Adult (18 – 23 g) Adv/Ano1fl/fl , and Ano1fl/fl mice were used for the behavioral tests. All experiments were performed during the day time between the hours (9:00 a.m. and 6:00 p.m.). Animals for all experiments were habituated before testing.
Spared-nerve injury (SNI)-induced neuropathic pain
Neuropathic pain was induced in mice with nerve injury as described . Briefly, mice were anesthetized by intraperitoneal (i.p.) injection of 50 mg/kg pentobarbital. We checked reflexes by pinching paws using a pincette to test whether mice were under anesthetic condition. An incision (~0.3 cm long) around the knee was made in the longitudinal direction. Nerve injury was induced through cutting tibial and common peroneal-nerve branches among three branches of the sciatic nerve.
Hargreaves plantar test
This test was performed using a standard apparatus (Ugo Basile Biological research Apparatus) as previously described . Briefly, a mouse was placed in a transparent acrylic box. An infrared heat lamp was positioned underneath the targeted hind paw. A radiant stimulus (intensity 50) was then applied to the plantar surface. Each test was repeated three times with 5 min interval. The paw withdrawal latency was calculated as the average of three values measured.
Von Frey hair test
Mechanical allodynia was measured by prodding the plantar region of the hindpaw with calibrated von Frey filaments (Stoelting Co., Wood Dale, IL). Animals were placed in cages with a mesh grid floor. On testing day, mice were allowed to acclimate for a minimum of 30 min before the experiment. Plantar surface of the hind foot was poked with Von Frey filaments of different thickness. Withdrawal thresholds to Von Frey filaments were determined when animals lifted their hindpaw at least 5 responses out of 10 stimulations. Minimum weight of the Von Frey filament that evoked a response was considered as a mechanical withdrawal threshold.
Bradykinin and formalin induced nocifensive behavior test
Mice were placed in a transparent acrylic box and allowed to acclimate for approximately 1 hr before injection. Nocifensive behaviors of mice were measured for 30 min after bradykinin injection. Formalin-induced behaviors were evaluated for 45 min being subdivided into two phases (phase I; 0-15 min, phase II; 15–45 min). Behaviors of mice such as biting, flinching or licking were considered as nocifensive behaviors.
To induce inflammation by carrageenan, carrageenan (2%) was injected subcutaneously into the plantar surface of left hind paws of mice (50 μl). Bradykinin (1 μg) and formalin (5%, 50 μl) were administered subcutaneously into plantar surfaces of left hind paws of control and Adv/Ano1 fl/fl mice. The inflammatory soup contained 10 μM of bradykinin, prostaglandin E2, serotonin, histamine, respectively. The inflammatory soup was applied to DRG neurons 2 hr before current clamp recordings. All chemicals were purchased from Sigma-Aldrich and dissolved in physiological saline.
Culture of primary DRG neuron
Primary cell cultures of DRG neurons were conducted as previously described . Thoracic and lumbar DRGs were dissected from control and Ano1/Adv fl/fl mice, and collected in cold culture medium (4°C) containing a mixture of DMEM and F-12 solution, 10% fetal bovine serum (Gibco BRL), 1 mM sodium pyruvate, 50–100 ng/ml nerve growth factor (Alomon, Jerusalem, Israel), and 100 units/ml of penicillin/streptomycin. Ganglia was washed with culture medium and incubated for 30 min in a warm (37°C) DMEM/F-12 mixture containing 1 mg/ml of collagenase (Type II; Worthington Biomedical). DRGs were then washed 3 times with Mg2+- and Ca2+-free Hank’s solution, and incubated while gently shaking in Hank’s solution containing 2.5 mg/ml of trypsin (Roche Diagnostics) for 30 min at 37°C. The trypsin-containing solution was then centrifuged at 100 g for 10 min. The pellets obtained after centrifugation were washed gently 2–3 times with culture medium and gently triturated with a fire-polished Pasteur pipette. Cells were plated onto round glass coverslips (Fisher), which had been previously treated with poly-L-lysine (0.5 mg/ml), in small Petri dishes (35 × 12 mm). Cells were then placed in a 37°C incubator in a 95% air/5% CO2 atmosphere. Cells were used 2–4 days after plating.
Current clamp recordings were performed using an Axopatch 200B (Molecular devices). Data was amplified, stored in a personal computer after digitization using Digidata 1440 (Molecular devices). Bath solution contained 140 mM NaCl, 5 mM KCl, 10 mM HEPES, 2 mM MgCl2, 2 mM CaCl2, adjusted to pH 7.2. Pipette solution contained 30 mM KCl, 100 mM K-aspartate, 10 mM HEPES, 1 mM MgCl2, 1 mM EGTA, and 0.225 mM CaCl2 adjusted to pH7.2. Whole cells were formed after breaking the plasma membrane under pipette tips. Resistance of the glass pipette was about 3 MΩ. Junctional potentials were cancelled to zero. The recordings were switched to the current clamp configuration. After a stable baseline was recorded, resting membrane potential (RMP) was measured in the DRG neurons of inflammatory soup treated group and vehicle treated group. The rheobase was recorded by an injection of current from 50 pA to 1 nA with 50 pA interval. Current injection with 1 nA magnitude was used to measure the latency to evoke first action potential spike.
All results are expressed as means ± SEMs and were analyzed by Student-T test. One way ANOVA was used for multiple comparison of the means followed by Duncan’s post-hoc test. Statistical significance was accepted for p values of < 0.05.
Reduction in inflammatory hyperalgesia and allodynia in Adv/Ano1fl/fl mice
To see if the ablation of Ano1 in DRG neurons also affects mechanical allodynia, the Von Frey-hair test was conducted after the injection of carrageenan to hind paws of Adv/Ano1 fl/fl mice. A significant drop (p < 0.01, one way-ANOVA, Duncan post-hoc test) in the withdrawal threshold for mechanical allodynia was observed in control mice from 30 min after carrageenan injection (Figure 1C). In contrast, this drop in the withdrawal threshold for mechanical allodynia after carrageenan injection was not observed in Adv/Ano1 fl/fl mice (p < 0.01 ~ 0.001, n = 6). Thus, Adv/Ano1 fl/fl mice exhibited significantly less thermal hyperalgesia and mechanical allodynia induced by inflammation.
ANO1 reduces nociception evoked by bradykinin and formalin
Formalin is also a well-known algogenic chemical that produces nocifensive responses. Formalin-induced nocifensive behaviors are subdivided into two phases [30, 31]. The first phase of nocifensive behaviors that lasts for 10 min after the formalin injection is caused by direct stimulation of sensory nerves whereas the second phase starting 10 min after formalin injection is mediated by inflammatory reaction [30, 31]. As shown in Figure 2B, ablation of Ano1 in DRG neurons is resulted in a significant decrease in the duration of nocifensive behaviors in the 2nd phase of nociception after formalin injection (230 ± 46 vs 123 ± 15.9 s, p < 0.05, n = 6–7). In contrast, Adv/Ano1 fl/fl mice failed to show the difference in the duration of nocifensive behaviors in the first phase (0–10 min) after formalin injection when compared to that of control mice. Thus, these results further indicate that ANO1 is involved in mediating BK- or formalin-induced inflammatory pain.
Reduction in neuropathic hyperalgesia and allodynia in Adv/Ano1fl/fl mice
Through the significant reduction (p < 0.01, one way-ANOVA, Duncan post-hoc test, n = 6) in the withdrawal threshold to Von Frey hairs after the nerve injury, mechanical allodynia was evident in ipsilateral hind paws of control mice after the nerve injury (Figure 3B). The reduction in the withdrawal threshold was not observed in contralateral hind paws of control mice. Significant mechanical allodynia (p < 0.01, one way-ANOVA, Duncan post-hoc test, n = 6) was also evident in ipsilateral hind paws of Adv/Ano1 fl/fl mice. However, the reduction in the withdrawal threshold after nerve injury was significantly smaller than that of control mice (p < 0.01 ~ 0.05, n = 5–6, Figure 3B). These results indicate that ANO1 plays a role in thermal hyperalgesia and mechanical allodynia induced by nerve injury.
ANO1 augments the excitability of DRG neurons
ANO1 is activated by intracellular Ca2+ and mediates many physiological functions as a candidate gene for Ca2+-activated Cl- channel [16–18, 20, 21, 36]. Mostly, ANO1 is present in nociceptors of DRG neurons [16, 23]. Remarkably, ANO1 is activated by heat over 44°C and mediates acute thermal pain, because Ano1-deficient mice displayed reduced nociceptive behaviors in response to heat . The present study extends its role from a heat transducer to a regulator of transmission of nociceptive signals evoked by inflammation or neuropathy. Indeed, ANO1 plays an active role in mediating inflammatory as well as neuropathic pain because inflammation or nerve-injury induced hyperalgesia or allodynia was reduced in Adv/Ano1 fl/fl mice. The decreased excitability in Adv/Ano1 fl/fl mice may account for the apparent reduced hyperalgesia and allodynia during inflammation. Thus, in addition to its ability of transducing acute noxious thermal stimuli to nociceptive neural signals, ANO1 is susceptible to intracellular signals that shape or affect transmission of nociceptive signals to the spinal cord during pathological conditions.
Thermal or mechanical hyperalgesia and allodynia are common features of neuropathic pain or inflammatory pain [1, 5]. Pathological pain occurs in response to various inflammatory mediators such as prostaglandins, histamine, BK, ATP, serotonin (5-HT), proton, cytokines, growth factors, and neuropeptides that are released from inflammed or injured tissues . These chemicals evoke peripheral sensitization, resulting in enhancement of the excitability of nociceptive nerve fibers. Several intracellular signaling pathways are known to cause the peripheral sensitization . Some inflammatory agents such as BK, prostaglandins, ATP or endothelin-1 activate various protein kinases and modulate channels via phosphorylation [39–42]. The target channels would be TRPV1, TRPA1, and Nav1.8 that are expressed in terminals of nociceptors [43, 44]. Similarly, ANO1 is highly expressed in nociceptors and activated by heat and also has multiple protein kinase consensus sites for protein kinase A, casein kinase, and other protein kinases [16, 23]. Because ANO1 regulates the inflammation-induced membrane excitability in DRG neurons (Figure 4), phosphorylation of ANO1 by several kinases may account for the change of excitability of sensory neurons caused by inflammation.
Many thermoTRP channels are activated by multiple stimuli such as their respective ligands, voltage, and changes in temperature, which often produce synergistic effect among each other on their activities [7, 45]. Responses of TRPV1 by heat and capsaicin were markedly potentiated by moderate acid that normally does not induce any TRPV1 current. In addition, TRPM3 has a strong synergistic effect between heat and pregnenolone sulfate, a neuroactive steroid . The temperature-response curve of TRPM3 was shifted leftward by pregnenolone sulfate. Similarly, activity of ANO1 by heat is markedly augmented by its endogenous ligand, Ca2+. The increase in intracellular Ca2+ enhances heat-evoked ANO1 currents, decreasing the temperature threshold of ANO1 below 44°C . For instance, when intracellular Ca2+ was increased above 0.5 μM, ANO1 was activated by the temperature close to body temperature, 37.5°C. Because inflamed tissues can cause the increase in intracellular Ca2+ [2, 46], it is conceivable that ANO1 can be sensitized by heat at body temperature under such a pathological condition.
It has been suggested that the regulation of intracellular Cl- concentration in sensory neurons is involved in the mediation of inflammatory hyperalgesia or transmission of nociceptive signals [47, 48]. Numerous studies have explained that the accumulation of intracellular Cl- by NKCC1 which cause Cl- uptake is correlated with pain behaviors. Nociceptive behaviors induced by formalin injection were significantly attenuated by NKCC1 blockers, bumetanide and furosemide . In addition, itch and flare responses by histamine injection were also reduced by these NKCC1 blockers [50, 51]. If intracellular Cl- concentration is increased in inflamed tissues, the driving force of Cl- to depolarization becomes greater when ANO1 is activated. Thus, an increase in intracellular Cl- concentration during inflammation would also contribute to the ANO1′s role in mediating chronic pain.
In summary, the present study revealed that Ano1 deletion in DRG neurons reduced inflammation and neuropathy-induced hyperalgesia and allodynia. In addition, the sensitization of DRG neurons induced by inflammation mediators was also absent in Ano1-ablated DRG neurons. These results suggest that ANO1 contributes to the sensitization of nociceptors during pathological conditions. The involvement of ANO1 in regulating the inflammatory or nerve-injury evoked pain may lead to the possibility that antagonists of ANO1 can be developed as novel analgesics.
This research was supported by a grant from the National Research Foundation of Korea (No. 20110018358) and a grant from BK21+ program of Ministry of Education of Korea.
- Basbaum AI, Bautista DM, Scherrer G, Julius D: Cellular and molecular mechanisms of pain. Cell 2009,139(2):267–284.PubMed CentralPubMedView ArticleGoogle Scholar
- Gangadharan V, Kuner R: Pain hypersensitivity mechanisms at a glance. Dis Model & Mech 2013,6(4):889–895.View ArticleGoogle Scholar
- Perl ER: Ideas about pain, a historical view. Nat Rev Neurosci 2007,8(1):71–80.PubMedView ArticleGoogle Scholar
- Patapoutian A, Tate S, Woolf CJ: Transient receptor potential channels: targeting pain at the source. Nat Rev Drug Discov 2009,8(1):55–68.PubMed CentralPubMedView ArticleGoogle Scholar
- Vay L, Gu C, McNaughton PA: The thermo-TRP ion channel family: properties and therapeutic implications. Br J Pharmacol 2012,165(4):787–801.PubMed CentralPubMedView ArticleGoogle Scholar
- Caterina MJ, Schumacher MA, Tominaga M, Rosen TA, Levine JD, Julius D: The capsaicin receptor: a heat-activated ion channel in the pain pathway. Nature 1997,389(6653):816–824.PubMedView ArticleGoogle Scholar
- Tominaga M, Caterina MJ, Malmberg AB, Rosen TA, Gilbert H, Skinner K, Raumann BE, Basbaum AI, Julius D: The cloned capsaicin receptor integrates multiple pain-producing stimuli. Neuron 1998,21(3):531–543.PubMedView ArticleGoogle Scholar
- Hwang SW, Cho H, Kwak J, Lee SY, Kang CJ, Jung J, Cho S, Min KH, Suh YG, Kim D, et al.: Direct activation of capsaicin receptors by products of lipoxygenases: endogenous capsaicin-like substances. Proc Natl Acad Sci U S A 2000,97(11):6155–6160.PubMed CentralPubMedView ArticleGoogle Scholar
- Caterina MJ, Leffler A, Malmberg AB, Martin WJ, Trafton J, Petersen-Zeitz KR, Koltzenburg M, Basbaum AI, Julius D: Impaired nociception and pain sensation in mice lacking the capsaicin receptor. Science 2000,288(5464):306–313.PubMedView ArticleGoogle Scholar
- Davis JB, Gray J, Gunthorpe MJ, Hatcher JP, Davey PT, Overend P, Harries MH, Latcham J, Clapham C, Atkinson K, et al.: Vanilloid receptor-1 is essential for inflammatory thermal hyperalgesia. Nature 2000,405(6783):183–187.PubMedView ArticleGoogle Scholar
- Bandell M, Story GM, Hwang SW, Viswanath V, Eid SR, Petrus MJ, Earley TJ, Patapoutian A: Noxious cold ion channel TRPA1 is activated by pungent compounds and bradykinin. Neuron 2004,41(6):849–857.PubMedView ArticleGoogle Scholar
- Bautista DM, Movahed P, Hinman A, Axelsson HE, Sterner O, Hogestatt ED, Julius D, Jordt SE, Zygmunt PM: Pungent products from garlic activate the sensory ion channel TRPA1. Proc Natl Acad Sci U S A 2005,102(34):12248–12252.PubMed CentralPubMedView ArticleGoogle Scholar
- Obata K, Katsura H, Mizushima T, Yamanaka H, Kobayashi K, Dai Y, Fukuoka T, Tokunaga A, Tominaga M, Noguchi K: TRPA1 induced in sensory neurons contributes to cold hyperalgesia after inflammation and nerve injury. J Clin Invest 2005,115(9):2393–2401.PubMed CentralPubMedView ArticleGoogle Scholar
- Katsura H, Obata K, Mizushima T, Yamanaka H, Kobayashi K, Dai Y, Fukuoka T, Tokunaga A, Sakagami M, Noguchi K: Antisense knock down of TRPA1, but not TRPM8, alleviates cold hyperalgesia after spinal nerve ligation in rats. Exp Neurol 2006,200(1):112–123.PubMedView ArticleGoogle Scholar
- Karashima Y, Talavera K, Everaerts W, Janssens A, Kwan KY, Vennekens R, Nilius B, Voets T: TRPA1 acts as a cold sensor in vitro and in vivo. Proc Natl Acad Sci U S A 2009,106(4):1273–1278.PubMed CentralPubMedView ArticleGoogle Scholar
- Yang YD, Cho H, Koo JY, Tak MH, Cho Y, Shim WS, Park SP, Lee J, Lee B, Kim BM, et al.: TMEM16A confers receptor-activated calcium-dependent chloride conductance. Nature 2008,455(7217):1210–1215.PubMedView ArticleGoogle Scholar
- Caputo A, Caci E, Ferrera L, Pedemonte N, Barsanti C, Sondo E, Pfeffer U, Ravazzolo R, Zegarra-Moran O, Galietta LJ: TMEM16A, a membrane protein associated with calcium-dependent chloride channel activity. Science 2008,322(5901):590–594.PubMedView ArticleGoogle Scholar
- Schroeder BC, Cheng T, Jan YN, Jan LY: Expression cloning of TMEM16A as a calcium-activated chloride channel subunit. Cell 2008,134(6):1019–1029.PubMed CentralPubMedView ArticleGoogle Scholar
- Huang F, Zhang H, Wu M, Yang H, Kudo M, Peters CJ, Woodruff PG, Solberg OD, Donne ML, Huang X, et al.: Calcium-activated chloride channel TMEM16A modulates mucin secretion and airway smooth muscle contraction. Proc Natl Acad Sci U S A 2012,109(40):16354–16359.PubMed CentralPubMedView ArticleGoogle Scholar
- Romanenko VG, Catalan MA, Brown DA, Putzier I, Hartzell HC, Marmorstein AD, Gonzalez-Begne M, Rock JR, Harfe BD, Melvin JE: Tmem16A encodes the Ca2 + -activated Cl- channel in mouse submandibular salivary gland acinar cells. J Biol Chem 2010,285(17):12990–13001.PubMed CentralPubMedView ArticleGoogle Scholar
- Hwang SJ, Blair PJ, Britton FC, O’Driscoll KE, Hennig G, Bayguinov YR, Rock JR, Harfe BD, Sanders KM, Ward SM: Expression of anoctamin 1/TMEM16A by interstitial cells of Cajal is fundamental for slow wave activity in gastrointestinal muscles. J Physiol 2009,587(Pt 20):4887–4904.PubMed CentralPubMedView ArticleGoogle Scholar
- Manoury B, Tamuleviciute A, Tammaro P: TMEM16A/anoctamin 1 protein mediates calcium-activated chloride currents in pulmonary arterial smooth muscle cells. J Physiol 2010,588(Pt 13):2305–2314.PubMed CentralPubMedView ArticleGoogle Scholar
- Cho H, Yang YD, Lee J, Lee B, Kim T, Jang Y, Back SK, Na HS, Harfe BD, Wang F, et al.: The calcium-activated chloride channel anoctamin 1 acts as a heat sensor in nociceptive neurons. Nat Neurosci 2012,15(7):1015–1021.PubMedView ArticleGoogle Scholar
- Decosterd I, Woolf CJ: Spared nerve injury: an animal model of persistent peripheral neuropathic pain. Pain 2000,87(2):149–158.PubMedView ArticleGoogle Scholar
- Hargreaves K, Dubner R, Brown F, Flores C, Joris J: A new and sensitive method for measuring thermal nociception in cutaneous hyperalgesia. Pain 1988,32(1):77–88.PubMedView ArticleGoogle Scholar
- Hasegawa H, Abbott S, Han BX, Qi Y, Wang F: Analyzing somatosensory axon projections with the sensory neuron-specific Advillin gene. J Neurosci 2007,27(52):14404–14414.PubMedView ArticleGoogle Scholar
- Keele CA: The chemistry of pain production. Proc R Soc Med 1967,60(4):419–422.PubMed CentralPubMedGoogle Scholar
- Dray A, Patel IA, Perkins MN, Rueff A: Bradykinin-induced activation of nociceptors: receptor and mechanistic studies on the neonatal rat spinal cord-tail preparation in vitro. Br J Pharmacol 1992,107(4):1129–1134.PubMed CentralPubMedView ArticleGoogle Scholar
- Liu B, Linley JE, Du X, Zhang X, Ooi L, Zhang H, Gamper N: The acute nociceptive signals induced by bradykinin in rat sensory neurons are mediated by inhibition of M-type K + channels and activation of Ca2 + -activated Cl- channels. J Clin Invest 2010,120(4):1240–1252.PubMed CentralPubMedView ArticleGoogle Scholar
- Tjolsen A, Berge OG, Hunskaar S, Rosland JH, Hole K: The formalin test: an evaluation of the method. Pain 1992,51(1):5–17.PubMedView ArticleGoogle Scholar
- Bolcskei K, Helyes Z, Szabo A, Sandor K, Elekes K, Nemeth J, Almasi R, Pinter E, Petho G, Szolcsanyi J: Investigation of the role of TRPV1 receptors in acute and chronic nociceptive processes using gene-deficient mice. Pain 2005,117(3):368–376.PubMedView ArticleGoogle Scholar
- Zhang X, Pietra C, Lovati E, de Groat WC: Activation of neurokinin-1 receptors increases the excitability of guinea pig dorsal root ganglion cells. J Pharmacol Exp Ther 2012,343(1):44–52.PubMed CentralPubMedView ArticleGoogle Scholar
- Huang F, Wang X, Ostertag EM, Nuwal T, Huang B, Jan YN, Basbaum AI, Jan LY: TMEM16C facilitates Na(+)-activated K + currents in rat sensory neurons and regulates pain processing. Nat Neurosci 2013,16(9):1284–1290.PubMed CentralPubMedView ArticleGoogle Scholar
- Maingret F, Coste B, Padilla F, Clerc N, Crest M, Korogod SM, Delmas P: Inflammatory mediators increase Nav1.9 current and excitability in nociceptors through a coincident detection mechanism. The Journal of General Physiology 2008,131(3)):211–225.PubMed CentralPubMedView ArticleGoogle Scholar
- Ma C, Greenquist KW, Lamotte RH: Inflammatory mediators enhance the excitability of chronically compressed dorsal root ganglion neurons. J Neurophysiol 2006,95(4):2098–2107.PubMedView ArticleGoogle Scholar
- Rock JR, O’Neal WK, Gabriel SE, Randell SH, Harfe BD, Boucher RC, Grubb BR: Transmembrane protein 16A (TMEM16A) is a Ca2 + -regulated Cl- secretory channel in mouse airways. J Biol Chem 2009,284(22):14875–14880.PubMed CentralPubMedView ArticleGoogle Scholar
- McMahon SB, Bennett DLH, Bevan S: Inflammatory mediators and modulators of pain. In Wall and Melzack's textbook of Pain. Edited by: McMahon SB, Koltzenburg M. Philadelphia: Elsevier; 2008:72.Google Scholar
- Gold MS, Gebhart GF: Nociceptor sensitization in pain pathogenesis. Nat Med 2010,16(11):1248–1257.PubMedView ArticleGoogle Scholar
- Vellani V, Mapplebeck S, Moriondo A, Davis JB, McNaughton PA: Protein kinase C activation potentiates gating of the vanilloid receptor VR1 by capsaicin, protons, heat and anandamide. J Physiol 2001,534(Pt 3):813–825.PubMed CentralPubMedView ArticleGoogle Scholar
- Bhave G, Hu HJ, Glauner KS, Zhu W, Wang H, Brasier DJ, Oxford GS, Gereau RW: Protein kinase C phosphorylation sensitizes but does not activate the capsaicin receptor transient receptor potential vanilloid 1 (TRPV1). Proc Natl Acad Sci U S A 2003,100(21):12480–12485.PubMed CentralPubMedView ArticleGoogle Scholar
- Moriyama T, Higashi T, Togashi K, Iida T, Segi E, Sugimoto Y, Tominaga T, Narumiya S, Tominaga M: Sensitization of TRPV1 by EP1 and IP reveals peripheral nociceptive mechanism of prostaglandins. Mol Pain 2005, 1: 3.PubMed CentralPubMedView ArticleGoogle Scholar
- Plant TD, Zollner C, Kepura F, Mousa SS, Eichhorst J, Schaefer M, Furkert J, Stein C, Oksche A: Endothelin potentiates TRPV1 via ETA receptor-mediated activation of protein kinase C. Mol Pain 2007, 3: 35.PubMed CentralPubMedView ArticleGoogle Scholar
- Chuang HH, Prescott ED, Kong H, Shields S, Jordt SE, Basbaum AI, Chao MV, Julius D: Bradykinin and nerve growth factor release the capsaicin receptor from PtdIns(4,5)P2-mediated inhibition. Nature 2001,411(6840):957–962.PubMedView ArticleGoogle Scholar
- Moriyama T, Iida T, Kobayashi K, Higashi T, Fukuoka T, Tsumura H, Leon C, Suzuki N, Inoue K, Gachet C, et al.: Possible involvement of P2Y2 metabotropic receptors in ATP-induced transient receptor potential vanilloid receptor 1-mediated thermal hypersensitivity. J Neurosci 2003,23(14):6058–6062.PubMedGoogle Scholar
- Vriens J, Owsianik G, Hofmann T, Philipp SE, Stab J, Chen X, Benoit M, Xue F, Janssens A, Kerselaers S, et al.: TRPM3 is a nociceptor channel involved in the detection of noxious heat. Neuron 2011,70(3):482–494.PubMedView ArticleGoogle Scholar
- Simonetti M, Hagenston AM, Vardeh D, Freitag HE, Mauceri D, Lu J, Satagopam VP, Schneider R, Costigan M, Bading H, et al.: Nuclear calcium signaling in spinal neurons drives a genomic program required for persistent inflammatory pain. Neuron 2013,77(1):43–57.PubMed CentralPubMedView ArticleGoogle Scholar
- Alvarez-Leefmans FJ, Leon-Olea M, Mendoza-Sotelo J, Alvarez FJ, Anton B, Garduno R: Immunolocalization of the Na(+)-K(+)-2Cl(-) cotransporter in peripheral nervous tissue of vertebrates. Neuroscience 2001,104(2):569–582.PubMedView ArticleGoogle Scholar
- Price TJ, Cervero F, Gold MS, Hammond DL, Prescott SA: Chloride regulation in the pain pathway. Brain Res Rev 2009,60(1):149–170.PubMed CentralPubMedView ArticleGoogle Scholar
- Granados-Soto V, Arguelles CF, Alvarez-Leefmans FJ: Peripheral and central antinociceptive action of Na + -K + -2Cl- cotransporter blockers on formalin-induced nociception in rats. Pain 2005,114(1–2):231–238.PubMedView ArticleGoogle Scholar
- Sung KW, Kirby M, McDonald MP, Lovinger DM, Delpire E: Abnormal GABAA receptor-mediated currents in dorsal root ganglion neurons isolated from Na-K-2Cl cotransporter null mice. J Neurosci 2000,20(20):7531–7538.PubMedGoogle Scholar
- Laird JM, Garcia-Nicas E, Delpire EJ, Cervero F: Presynaptic inhibition and spinal pain processing in mice: a possible role of the NKCC1 cation-chloride co-transporter in hyperalgesia. Neurosci Lett 2004,361(1–3):200–203.PubMedView ArticleGoogle Scholar
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